| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
Elucidation of the pore forming mechanism of hemolytic lectin CEL-III was done. Tertiary structure of the pore forming CEL-III showed that the pore region is consist of stem, wrapping and bundle region. Deletion mutants that lost stem region, stem and wrapping region were made and quaternary structures were analyzed by small-angle x-ray scattering. The results showed that the stem deleted mutant formed heptameric form and stem and wrapping region deleted mutant exist as monomer. This result indicates that the oligomerization is not depend on the stem region, and wrapping region affect the oligomerization. This suggests that the CEL-III forms heptamer at first on the surface of the cell membrane, and then forms pore in the cell membrane.
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