| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
Plants produce a variety of specialized metabolites. Many of them are used as raw materials for pharmaceuticals due to their useful biological activity. We have been investigating transporters involved in the efficient production of specialized metabolites, and in this study we focused on a plastid-localized uracil transporter, whose expression is induced similarly to those of tobacco alkaloid biosynthetic enzymes. Tobacco hairy roots and cultured cells overexpressing this transporter were produced and metabolites were comprehensively analyzed. The data suggested an increase or decrease in multiple amino acids, such as phenylalanine, as well as altered alkaloid productivity. These results revealed for the first time the role of uracil transporters in vivo and provide important insights that lead to efficient specialized metabolites production.
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