Bioluminescence reaction mechanism of luminous mushroom

• Project/Area Number: 16K07715
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Bioorganic chemistry
• Research Institution: Chubu University
• Principal Investigator: OBA Yuichi 中部大学, 応用生物学部, 准教授 (40332704)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 生物発光 / キノコ / ルシフェリン / ルシフェラーゼ / 発光キノコ / ルシフェリン酸化物 / 発光反応 / 天然物化学

Outline of Final Research Achievements

We successfully determined the chemical structure of the oxyluciferin and luciferase gene involving in the bioluminescence of luminous mushroom. We also found the recycling mechanism of luciferin from oxyluciferin via caffeic acid.

Academic Significance and Societal Importance of the Research Achievements

発光キノコの発光メカニズムが明らかになったことで、発光イメージングツールとしての基礎科学への応用可能性が開かれた。同時に発見されたルシフェリン生合成酵素とルシフェリン再生酵素を異種発現（発光しない生物に発光キノコの発光に関連するこれらの遺伝子を導入する）ことで、本来発光しない生物を緑色に発光させることができると考えられる。

Research Products

• [Int'l Joint Research] ロシア科学アカデミー/シベリア連邦大学(ロシア連邦)
• [Int'l Joint Research] サンパウロ大学(ブラジル)
• [Int'l Joint Research] ロシア科学アカデミー/シベリア連邦大学(ロシア連邦)
• [Int'l Joint Research] サンパウロ大学(ブラジル)
• [Int'l Joint Research] ロシア科学アカデミー(ロシア連邦)
• [Int'l Joint Research] サンパウロ大学(ブラジル)
• [Journal Article] Genetically encodable bioluminescent system from fungi (2018)
  • Author(s): Kotlobay, A.A., Sarkisyan, K.S., Oba, Y., Barsova, E., Bogdanova, E.A., Gabaldon, T., Stevani, C.V., Lukyanov, S., Smirnov, I.V., Gitelson, J.I., Kondrashov, F.A., Yampolsky, I.V. et al.
  • Journal Title: Proceedings of the National Academy of Sciences Volume: 115 Issue: 50 Pages: 12728-12732
  • DOI: 10.1073/pnas.1803615115
  • Peer Reviewed
• [Journal Article] Mechanism and color modulation of fungal bioluminescence (2017)
  • Author(s): Kaskova, Z., Dorr, F., Petushkov, V., Purtov, K., Tsarkova, A., Rodionova, N., Mineev, K., Guglya, E., Kotlobay, A., Baleeva, N., Baranov, M., Areseniev, A., Gitelson, J., Lukyanov, S., Suzuki, Y., Kanie, S., Pinto, E., Mascio, P. D., Waldenmaier, H., Pereira, T., Carvalho, R., Oliveira, A., Oba, Y., et al.
  • Journal Title: Science Advances Volume: 3 Issue: 4
  • DOI: 10.1126/sciadv.1602847
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Identification of hispidin as a bioluminescent active compound and its recycling biosynthesis in the luminous fungal fruiting body (2017)
  • Author(s): Yuichi Oba, Yoshiki Suzuki, Gabriel N. R. Martins, Rodrigo P. Carvalho, Tatiana A. Pereira, Hans E. Waldenmaier, Shusei Kanie, Masashi Naito, Anderson G. Oliveira, Felipe A. D, Ernani Pinto, Ilia V. Yampolsky, Cassius V. Stevani
  • Journal Title: Photochemical & Photobiological Sciences Volume: 16 Issue: 9 Pages: 1435-1440
  • DOI: 10.1039/c7pp00216e
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Selected least studied but not forgotten bioluminescent systems (2016)
  • Author(s): Oba Y, Stevani CV, Oliveira AG, Tsarkova AS, Chepurnykh TV, Yampolsky IV
  • Journal Title: Photochemistry and Photobiology Volume: 93 Issue: 2 Pages: 405-415
  • DOI: 10.1111/php.12704
  • Peer Reviewed / Int'l Joint Research
• [Presentation] Recycling system of hispidin in luminous mushroom (2018)
  • Author(s): Y. Oba, Y. Suzuki, G.N.R. Martin, R.P. Carvalho, T.A. Pereira, H.E. Waldenmaier, S. Kanie, M. Naito A.G. Oliveira, F.A. Dorr, E. Pinto, I.V. Yampolsky, and C.V. Stevani
  • Organizer: 20th International Symposium on Bioluminescence and Chemiluminescence
  • Int'l Joint Research
• [Presentation] 発光生物の科学、最近の話題 (2016)
  • Author(s): 大場裕一
  • Organizer: 化学生態学研究会
  • Place of Presentation: 函館市湯の川プリンスホテル（北海道・函館市）
  • Invited
• [Presentation] Evolution of bioluminescent organisms (2016)
  • Author(s): Yuichi Oba
  • Organizer: International Congress of Zoology
  • Place of Presentation: 沖縄コンベンションセンター（沖縄県・宜野湾市）
  • Int'l Joint Research / Invited
• [Book] 恐竜はホタルを見たか (2016)
  • Author(s): 大場裕一
  • Total Pages: 128
  • Publisher: 岩波書店