Studies on natural variation in defensive secondary metabolites for breeding of disease resistant cultivars
| Project/Area Number |
16K07718
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Bioorganic chemistry
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| Research Institution | Tottori University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
寺石 政義 京都大学, 農学研究科, 講師 (80378819)
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| Project Period (FY) |
2016-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2019: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | イネ / ファイトアレキシン / 種内多様性 / ケモタイプ / サクラネチン / オリザレシン / 二次代謝 / rice / phytoalexin / natural variaton / sakuranetin / oryzalexin / chemotype / momilactone / phytocassane / オリザレキシン / ナチュラルバリエーション / モミラクトン / フェニルアミド / ナリンゲニン / QTL |
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| Outline of Final Research Achievements |
Rice plant accumulate phytoalexins for preventing of pathogen infection. We investigated the natural variation in the accumulation of the flavonoid phytoalexin sakuranetin in rice. Among the cultivars from various regions in the world, there were sakuranetin accumulating and naringenin accumulating cultivars. Naringenin is the immediate precursor of sakuranetin. The causal gene for this difference was the sakuranetin biosynthetic gene NOMT. Sakuranetin showed strong antifungal activity, while naringenin showed strong antibacterial activity. This difference in antimicrobial activity may be the driving force for the development of different chemotypes. We also found the natural variation in the accumulation of the diterpenoid phytoalexin oryzalexin A. The causal gene of the different chemotypes was again the biosynthetic gene KSL10.
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| Academic Significance and Societal Importance of the Research Achievements |
植物は外敵に対する防御のため、さまざまな化学物質を蓄積します。例えば、病原菌が感染するとファイトアレキシンと呼ばれる抗菌性物質を蓄積します。イネは20種におよぶファイトアレキシンを蓄積するのですが、品種によってファイトアレキシンの種類や蓄積量が大きく異なることがわかりました。この結果を利用すれば、交配育種などの方法でファイトアレキシンの組成を操作することができ、特定の病原菌に強い品種を作り出せると考えられます。また、異なるファイトアレキシンを蓄積する系統を同時に栽培することで病気の広がりを抑えることができる可能性が生まれました。
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Report
(5 results)
Research Products
(24 results)
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[Journal Article] Natural variation in the expression and catalytic activity of a naringenin 7-O-methyltransferase influences antifungal defenses in diverse rice cultivars2020
Author(s)
Koichi Murata, Takashige Kitano, Riko Yoshimoto, Ryo Takata, Naoki Ube, Kotomi Ueno, Makoto Ueno, Yukinori Yabuta, Masayoshi Teraishi, Cynthia K. Holland, Georg Jander, Yutaka Okumoto, Naoki Mori, Atsushi Ishihara
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Journal Title
The Plant Journal
Volume: 101
Issue: 5
Pages: 1103-1117
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Induced accumulation of tyramine, serotonin, and related amines in response to Bipolaris sorokiniana infection in barley.2017
Author(s)
Atsushi Ishihara, Rie Kumeda, Noriko Hayashi, Yukari Yagi, Nanase Sakaguchi, Yu Kokubo, Naoki Ube, Shin-ichi Tebayashi, Kotomi Ueno
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Journal Title
Bioscience, Biotechnology, and Biochemistry
Volume: 印刷中
Issue: 6
Pages: 1090-1098
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] Natural variation in the expression and catalytic activity of a naringenin 7-O-methyltransferase influence antifungal defenses in diverse rice cultivars2019
Author(s)
Koichi Murata, Ryo Takata, Naoki Ube, Kotomi Ueno, Masayoshi Teraishi, Yutaka Okumoto, Naoki Mori, Atsushi Ishihara
Organizer
International Symposium on Agricultural, Food, Environmental and Life Sciences in Asia, 2019 (The 16th International Joint Symposium between Korea and Japan)
Related Report
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[Presentation] Natural variation of phytoalexin sakuranetin production in rice cultivars.2017
Author(s)
Takashige Kitano, Noriko Morimoto, Riko Yoshimoto, Sayaka Nishiguchi, Koichi Murata, Kotomi Ueno, Makoto Ueno, Yukinori Yabuta, Masayoshi Teraishi, Georg Jander, Yutaka Okumoto, Atsushi Ishihara
Organizer
The joint meeting of the 33rd Annual meeting of International Society of Chemical Ecology and the 9th meeting of the Asia Pacific Association of Chemical Ecology.
Related Report
Int'l Joint Research
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