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Studies on natural variation in defensive secondary metabolites for breeding of disease resistant cultivars

Research Project

Project/Area Number 16K07718
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Bioorganic chemistry
Research InstitutionTottori University

Principal Investigator

ISHIHARA Atsushi  鳥取大学, 農学部, 教授 (80281103)

Co-Investigator(Kenkyū-buntansha) 寺石 政義  京都大学, 農学研究科, 講師 (80378819)
Project Period (FY) 2016-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2019: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordsイネ / ファイトアレキシン / 種内多様性 / ケモタイプ / サクラネチン / オリザレシン / 二次代謝 / rice / phytoalexin / natural variaton / sakuranetin / oryzalexin / chemotype / momilactone / phytocassane / オリザレキシン / ナチュラルバリエーション / モミラクトン / フェニルアミド / ナリンゲニン / QTL
Outline of Final Research Achievements

Rice plant accumulate phytoalexins for preventing of pathogen infection. We investigated the natural variation in the accumulation of the flavonoid phytoalexin sakuranetin in rice. Among the cultivars from various regions in the world, there were sakuranetin accumulating and naringenin accumulating cultivars. Naringenin is the immediate precursor of sakuranetin. The causal gene for this difference was the sakuranetin biosynthetic gene NOMT. Sakuranetin showed strong antifungal activity, while naringenin showed strong antibacterial activity. This difference in antimicrobial activity may be the driving force for the development of different chemotypes. We also found the natural variation in the accumulation of the diterpenoid phytoalexin oryzalexin A. The causal gene of the different chemotypes was again the biosynthetic gene KSL10.

Academic Significance and Societal Importance of the Research Achievements

植物は外敵に対する防御のため、さまざまな化学物質を蓄積します。例えば、病原菌が感染するとファイトアレキシンと呼ばれる抗菌性物質を蓄積します。イネは20種におよぶファイトアレキシンを蓄積するのですが、品種によってファイトアレキシンの種類や蓄積量が大きく異なることがわかりました。この結果を利用すれば、交配育種などの方法でファイトアレキシンの組成を操作することができ、特定の病原菌に強い品種を作り出せると考えられます。また、異なるファイトアレキシンを蓄積する系統を同時に栽培することで病気の広がりを抑えることができる可能性が生まれました。

Report

(5 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (24 results)

All 2020 2019 2018 2017 Other

All Int'l Joint Research (3 results) Journal Article (6 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 6 results,  Open Access: 3 results,  Acknowledgement Compliant: 1 results) Presentation (15 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results)

  • [Int'l Joint Research] Boyce Thompson Institute(米国)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] Boyce Thompson Institute(米国)

    • Related Report
      2018 Research-status Report
  • [Int'l Joint Research] Boyce Thompson Institute(米国)

    • Related Report
      2017 Research-status Report
  • [Journal Article] Natural variation in the expression and catalytic activity of a naringenin 7-O-methyltransferase influences antifungal defenses in diverse rice cultivars2020

    • Author(s)
      Koichi Murata, Takashige Kitano, Riko Yoshimoto, Ryo Takata, Naoki Ube, Kotomi Ueno, Makoto Ueno, Yukinori Yabuta, Masayoshi Teraishi, Cynthia K. Holland, Georg Jander, Yutaka Okumoto, Naoki Mori, Atsushi Ishihara
    • Journal Title

      The Plant Journal

      Volume: 101 Issue: 5 Pages: 1103-1117

    • DOI

      10.1111/tpj.14577

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Atsushi Ishihara. Variation of diterpenoid phytoalexin oryzalexin A production in cultivated and wild rice2019

    • Author(s)
      Keisuke Kariya, Koichi Murata, Yu Kokubo, Naoki Ube, Kotomi Ueno, Yukinori Yabuta, Masayoshi Teraishi, Yutaka Okumoto, Naoki Mori, Atsushi Ishihara
    • Journal Title

      Phytochemistry

      Volume: 166 Pages: 112057-112057

    • DOI

      10.1016/j.phytochem.2019.112057

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Induced phenylamide accumulation in response to pathogen infection and hormone treatment in rice (Oryza sativa)2018

    • Author(s)
      Morimoto Noriko、Ueno Kotomi、Teraishi Masayoshi、Okumoto Yutaka、Mori Naoki、Ishihara Atsushi
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 82 Issue: 3 Pages: 407-416

    • DOI

      10.1080/09168451.2018.1429889

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Evolutionary changes in defensive specialized metabolism in the genus Hordeum2017

    • Author(s)
      Ube Naoki、Nishizaka Miho、Ichiyanagi Tsuyoshi、Ueno Kotomi、Taketa Shin、Ishihara Atsushi
    • Journal Title

      Phytochemistry

      Volume: 141 Pages: 1-10

    • DOI

      10.1016/j.phytochem.2017.05.004

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Distribution of the tryptophan-pathway-derived defensive secondary metabolites gramine and benzoxazinones in Poaceae.2017

    • Author(s)
      Kokubo Y, Nishizaka M, Ube N, Yabuta Y Tebayashi SI, Ueno K, Taketa S, Ishihara A
    • Journal Title

      Biosci Biotechnol Biochem.

      Volume: 81 Issue: 3 Pages: 431-440

    • DOI

      10.1080/09168451.2016.1256758

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Induced accumulation of tyramine, serotonin, and related amines in response to Bipolaris sorokiniana infection in barley.2017

    • Author(s)
      Atsushi Ishihara, Rie Kumeda, Noriko Hayashi, Yukari Yagi, Nanase Sakaguchi, Yu Kokubo, Naoki Ube, Shin-ichi Tebayashi, Kotomi Ueno
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 印刷中 Issue: 6 Pages: 1090-1098

    • DOI

      10.1080/09168451.2017.1290520

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] イネにおけるテルペノイド型ファイトアレキシン生産の種内多様性2020

    • Author(s)
      假谷 佳祐、宇部 尚樹、上野 琴巳、寺石 政義、奥本 裕、森 直樹、石原 亨
    • Organizer
      日本農芸化学会2020年度大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] イネのインディカ亜種に特異的に蓄積するファイトアンティシピン2020

    • Author(s)
      村田晃一、宇部尚樹、寺石政義、上野誠、上野琴巳、奥本裕、森直樹、石原亨
    • Organizer
      日本農薬学会第45回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] イネにおけるフラボノイド型ファイトアレキシン、サクラネチンの蓄積に関する種内多様性2019

    • Author(s)
      村田 晃一、高田 諒、宇部 尚樹、上野 琴巳、寺石 政義、奥本 裕、森 直樹、石原 亨
    • Organizer
      植物化学調節学会第54回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] イネにおけるテルペノイド型ファイトアレキシン生産の種内多様性2019

    • Author(s)
      假谷佳祐、宇部尚樹、上野琴巳、寺石政義、奥本裕、森直樹、石原亨
    • Organizer
      植物化学調節学会第54回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Natural variation in the expression and catalytic activity of a naringenin 7-O-methyltransferase influence antifungal defenses in diverse rice cultivars2019

    • Author(s)
      Koichi Murata, Ryo Takata, Naoki Ube, Kotomi Ueno, Masayoshi Teraishi, Yutaka Okumoto, Naoki Mori, Atsushi Ishihara
    • Organizer
      International Symposium on Agricultural, Food, Environmental and Life Sciences in Asia, 2019 (The 16th International Joint Symposium between Korea and Japan)
    • Related Report
      2019 Annual Research Report
  • [Presentation] イネにおけるフラボノイド型ファイトアレキシン、サクラネチンの蓄積に関する種内変異2019

    • Author(s)
      村田晃一、高田諒、宇部尚樹、上野琴巳、寺石政義、奥本裕、森直樹、石原亨
    • Organizer
      日本農薬学会第44回大会
    • Related Report
      2018 Research-status Report
  • [Presentation] イネにおけるテルペノイド型ファイトアレキシン、オリザレキシンAの蓄積に関する種内変異2019

    • Author(s)
      假谷佳祐,村田晃一,宇部尚樹, 上野琴巳, 寺石政義, 奥本裕, 森直樹, 石原亨
    • Organizer
      日本農薬学会第44回大会
    • Related Report
      2018 Research-status Report
  • [Presentation] イネにおけるフラボノイド型ファイトアレキシン、サクラネチンの蓄積に関するナチュラルバリエーション2019

    • Author(s)
      村田晃一、高田諒、宇部尚樹、上野琴巳、寺石政義、奥本裕、森直樹、石原亨
    • Organizer
      日本農芸化学会2019年度大会
    • Related Report
      2018 Research-status Report
  • [Presentation] イネにおけるテルペノイド型ファイトアレキシン、オリザレキシンAの蓄積に関するナチュラルバリエーション2019

    • Author(s)
      假谷佳祐、村田晃一、宇部尚樹、上野琴巳、寺石政義、奥本裕、森直樹、石原亨
    • Organizer
      日本農芸化学会2019年度大会
    • Related Report
      2018 Research-status Report
  • [Presentation] イネのファイトアレキシン生産における種内多様性2018

    • Author(s)
      小久保悠、村田晃一、假谷佳祐、上野琴巳、寺石政義、奥本裕、森直樹、石原亨
    • Organizer
      日本農芸化学会2018年度大会
    • Related Report
      2017 Research-status Report
  • [Presentation] 病原菌感染や植物ホルモン処理がイネにおけるフェニルアミド類の蓄積に及ぼす影響2017

    • Author(s)
      森本紀子、上野琴巳、寺石政義、奥本裕、森直樹、石原亨
    • Organizer
      日本農芸化学会2017年度大会
    • Place of Presentation
      京都市
    • Year and Date
      2017-03-17
    • Related Report
      2016 Research-status Report
  • [Presentation] イネ科植物における二次代謝多様性の進化2017

    • Author(s)
      石原亨
    • Organizer
      日本農芸化学会2017年度大会
    • Place of Presentation
      京都市
    • Year and Date
      2017-03-17
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] イネ科植物におけるジャスモン酸処理によるフェニルアミドの誘導2017

    • Author(s)
      森本紀子,上野琴巳,寺石政義,奥本裕,森直樹,石原亨
    • Organizer
      日本農芸化学会中四国支部第47回講演会
    • Place of Presentation
      松江市
    • Year and Date
      2017-01-28
    • Related Report
      2016 Research-status Report
  • [Presentation] Natural variation of phytoalexin sakuranetin production in rice cultivars.2017

    • Author(s)
      Takashige Kitano, Noriko Morimoto, Riko Yoshimoto, Sayaka Nishiguchi, Koichi Murata, Kotomi Ueno, Makoto Ueno, Yukinori Yabuta, Masayoshi Teraishi, Georg Jander, Yutaka Okumoto, Atsushi Ishihara
    • Organizer
      The joint meeting of the 33rd Annual meeting of International Society of Chemical Ecology and the 9th meeting of the Asia Pacific Association of Chemical Ecology.
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] イネにおける植物ホルモン処理によるフェニルアミド類の誘導2017

    • Author(s)
      森本紀子、上野琴巳、寺石政義、奥本裕、森直樹、石原亨
    • Organizer
      日本農芸化学会関西・中四国・西日本支部2017年度合同大阪大会
    • Related Report
      2017 Research-status Report

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Published: 2016-04-21   Modified: 2022-02-21  

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