| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2018: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2016: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Outline of Final Research Achievements |
Simultaneous determination method of eight homologues of vitamin E was established by using reversed-phase HPLC under low column temperature conditions. By this method, vitamin E homologues could be separated and quantified within 20 minutes. In addition, the influence of the peaks that interfered the analysis of tocotrienols in some samples could be eliminated by the 2D-HPLC using low column temperature separation system.
On the other hand, the low temperature separation HPLC method was also applied to the stereoisomer composition analysis of a novel alkanoylimidazole compound isolated from gammaproteobacterium Microbulbifer sp. which has an anteiso branched methyl group. As the results, the stereochemistry of the anteiso branched methyl was found that the S-form was the main stereoisomer (about 91%) but about 9% of the R-form was also contained.
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