Biological activities of multifunctional protein in milk and their applications
| Project/Area Number |
16K07745
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Food science
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| Research Institution | Kyoritsu Women's University |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2020: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2019: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 食品機能 / 鉄代謝 / 唾液タンパク質 / アフィニティー精製 / 食品 / 蛋白質 / 生理活性 |
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| Outline of Final Research Achievements |
We investigated the possibility that, besides the multi-functionality derived from the lactoferrin (LF) itself, LF expressed behaviors in conjunction with other bioactive compounds. The proteins and peptides present in LF used as a reagent and food ingredient were examined using LC-MS/MS proteomic analysis. Impurities in LF were fractionated using multi-stage methods, including affinity chromatography and ion exchange chromatography, and their bioactivities were individually evaluated by in vitro and in vivo experiments. Several components that exhibit bioactive characteristics, including immunomodulating effects and iron delivery metabolism were present among the impurities. The multifunctional properties of LF were suggested to be due to some compounds interacted with LF.
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| Academic Significance and Societal Importance of the Research Achievements |
ラクトフェリン（LF）は、免疫調節作用、抗ウイルス作用、抗菌作用、鉄代謝調節作用、骨代謝調節作用、内臓脂肪低減作用など、20種類以上の生理作用をもつことが報告されている。単一物質としては生理作用の多様性が極めて高く、乳の一成分でありながら PubMedで文献検索を行うと、現在までに8,000報以上の様々な研究報告がある。そこで、LFの多種多様な生理作用が、LFと特異的に結合している複数の生理活性成分に起因するのではないかという仮説を立てて研究を行い、LFが保有するいくつかの生理作用の活性本体を明らかにした。本研究は今後のLF研究の方向性を示唆し、革新的な進展をもたらすものと思われる。
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Report
(6 results)
Research Products
(12 results)
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[Journal Article] Lactoferrin Suppresses Neutrophil Extracellular Traps Release in Inflammation.2016
Author(s)
Okubo K, Kamiya M, Urano Y, Nishi H, Herter JM, Mayadas T, Hirohama D, Suzuki K, Kawakami H, Tanaka M, Kurosawa M, Kagaya S, Hishikawa K, Nangaku M, Fujita T, Hayashi M, Hirahashi J.
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Journal Title
EBioMedicine
Volume: 10
Pages: 204-215
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Presentation] Analyzing the molecular mechanism of inhibition of bovine norovirus infection by bovine lactoferrin2018
Author(s)
Hu, C., Shojima, T., Katayama, Y., Suzuki, M., Kawakami, H., Nagata, K.
Organizer
日本農芸化学会
Related Report
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[Presentation] Screening for infection inhibitors of human norovirus GII.17 from food ingredients2018
Author(s)
Li, L., Shojima, T., Lu, L., Katayama, Y., Suzuki, M., Kawakami, H., Nagata, K
Organizer
日本農芸化学会
Related Report
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[Book] ヒトの基礎生化学2021
Author(s)
川上 浩
Total Pages
259
Publisher
アイ・ケイコーポレーション
ISBN
9784874923320
Related Report
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