Construction of enzyme development method for food industry using Bacillus subtilis var. natto.
| Project/Area Number |
16K07762
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Food science
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| Research Institution | Saitama Institute of Technology |
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Principal Investigator |
HATADA Yuji 埼玉工業大学, 工学部, 教授 (20399562)
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| Project Period (FY) |
2016-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 納豆菌 / プラスミドDNA / 遺伝子 / 酵素生産 / 形質転換 / プラスミド / ベクター / 遺伝子導入 / シャトルベクター / 染色体DNA相同性組換え / GFP / 発現ベクター / 酵素 / 応用微生物 / 食品 |
|---|
| Outline of Final Research Achievements |
A plasmid DNA (named pTHK) was discovered from a Bacillus subtilis var. natto. Then, DNA fragments of various regions from pTHK were amplified by PCR, ligated with an antibiotic resistance gene, and introduced into the Bacillus natto. Based on these results, the plasmid was miniaturized, and we succeeded in obtaining a plasmid that was miniaturized to about 60% of the size of the original plasmid. Advantageously, the miniaturized plasmid had a higher efficiency of transformation of Bacillus natto than the original plasmid. By applying the minimized plasmid, we succeeded in constructing the shuttle vector of Bacillus natto ⇔ E. coli. We have also succeeded in introducing foreign genes into Bacillus natto.
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| Academic Significance and Societal Importance of the Research Achievements |
醗酵食品を古（いにしえ）より食する日本において、納豆菌は食経験歴が長く、それ故に安全性も担保されている。納豆菌を用いて、食品産業用酵素の新生産システムを開発し、新規酵素の開発の高速度化を目指す。本研究の研究成果は、その新生産システムの土台の構築に大きく貢献する多くの新たな有益な知見を含んでいる。
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Report
(5 results)
Research Products
(8 results)
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[Presentation] Droplet-based microfluidic screening for obtaining microbes producing macromolecule-degrading enzymes2018
Author(s)
Ryo Iizuka, Kai Saito, Eiji Shigihara, Wataru Kawakubo, Daiki Tanaka, Dong Hyun Yoon, Tetsushi Sekiguchi, Shuichi Shoji, Mei Ito, Yuji Hatada, Takashi Funatsu
Organizer
第56回日本生物物理学会年会
Related Report
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[Book] 食と微生物の事典2017
Author(s)
北本勝ひこ等編、秦田勇二、大田ゆかり等
Total Pages
512
Publisher
朝倉書店
ISBN
9784254431216
Related Report
