Research for preventing Japanese oak wilt (JOW) disease with tree endophyte fungi

Research Project

Project/Area Number	16K07766
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Forest science
Research Institution	Yamagata University
Principal Investigator	SHIONO YOSHIHITO 山形大学, 農学部, 教授 (80361278)
Project Period (FY)	2016-04-01 – 2019-03-31
Project Status	Completed (Fiscal Year 2018)
Budget Amount *help	¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000) Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000) Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000) Fiscal Year 2016: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords	ナラ菌 / Raffaelea quercivora / Scytalone / 植物内生菌 / 植物毒性 / ナラ枯れ / シタロン / 抗菌活性 / scytalone / 微生物 / 里山 / 生理活性物質 / 森林
Outline of Final Research Achievements	Among the components contained in phytotoxic substances and metabolites of Raffaelea quercivora, it was aimed to clarify the characteristic toxicity to plants and characteristic metabolites contained in culture extract. As a result, it was found that a new substance having dioxotetrahydrofuro [3,4-b] furan skeleton was produced under the condition where sawdust of oak tree was added to the medium. It also showed inhibitory activity against lettuce root.
Academic Significance and Societal Importance of the Research Achievements	これまでに、明らかになっていないナラ菌の生産する植物毒性物質を探索することであり、明らかになった物質の構造から、生理活性試験や活性の予測を行い、ナラ枯れの萎凋メカニズムを解明する一つの手がかりとする。さらに、ナラ菌の生産する植物毒性物質の生産を抑制する作用を有する菌類を樹木内生菌類に求め探索し、新たなナラ枯れ抑制方法を提案することができる。