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Symbiotic nitrogen fixing microorganisms in Sphagnum plants and their relation to photosynthesis

Research Project

Project/Area Number 16K07784
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Forest science
Research InstitutionThe University of Kitakyushu

Principal Investigator

Haraguchi Akira  北九州市立大学, 国際環境工学部, 教授 (50271630)

Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords泥炭形成植物 / 共生微生物 / 窒素固定 / 安定同位体比 / 光合成 / ミズゴケ / 共生バクテリア / ユーグレナ / 群落光合成 / シアノバクテリア / 生理生態 / 泥炭 / 物質循環
Outline of Final Research Achievements

Symbiotic microorganisms were selected from Sphagnum plants collected from 6 populations from Hokkaido to Kyushu. Extracts of Sphagnum plants were cultured on plate culture medium without nitrogen and four genus of bacteria; Pandoraea, Sphingomonas, Paraburkholderia, Burkholderia and Coccomyxa (Trebouxiophyceae, Eukaryota) were isolated. One species of genus Euglena was also isolated from one population of Sphagnum. Nitrogen stable isotope ratio showed specific value for localities; δ15N for populations in Kyushu showed around 0 and δ15N showed decreasing tendency with increasing latitude. However, evidence of nitrogen fixation by isolated symbiotic microorganisms has never been obtained from stable isotope analysis.

Academic Significance and Societal Importance of the Research Achievements

ミズゴケ類は北方泥炭地の主要な泥炭形成植物であり、全球の炭素循環において重要な役割を担っているが、一次生産機能に関してはまだ十分に解析されていない。本研究の中で、ミズゴケ類の光合成曲線が顕著な季節変動を示すことがわかり、今後ミズゴケの光合成機能やミズゴケ群集の一次生産を精緻に解析するうえで季節変動が重要な要素であることが示された。共生微生物に関しては、無窒素培地での培養から5属の微生物が単離され、これらは窒素固定にかかわるものと考えられるため、ミズゴケ群集の育成においての活用が期待できる。また、ミズゴケ共生微生物として、Euglena属の種が国産のミズゴケ類については初めて確認された。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (14 results)

All 2018 2017 2016 Other

All Int'l Joint Research (4 results) Journal Article (9 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 8 results,  Open Access: 3 results) Presentation (1 results) (of which Int'l Joint Research: 1 results)

  • [Int'l Joint Research] 東フィンランド大学(フィンランド)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] ウプサラ大学(スウェーデン)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] 東フィンランド大学(フィンランド)

    • Related Report
      2017 Research-status Report
  • [Int'l Joint Research] 東フィンランド大学(フィンランド)

    • Related Report
      2016 Research-status Report
  • [Journal Article] Environmental and taxonomic controls of carbon and oxygen stable isotope composition in Sphagnum across broad climatic and geographic ranges.2018

    • Author(s)
      Granath G ... Haraguchi A...et al.
    • Journal Title

      Biogeosciences

      Volume: 15 Issue: 16 Pages: 5189-5202

    • DOI

      10.5194/bg-15-5189-2018

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Effect of salinity on seed germination and seedling growth of the halophyte Suaeda japonica Makino.2018

    • Author(s)
      Haraguchi A, Matsuda T
    • Journal Title

      Plant Species Biology

      Volume: 33 Issue: 3 Pages: 229-235

    • DOI

      10.1111/1442-1984.12211

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Biologically driven DOC release from peatlands during recovery from acidification.2018

    • Author(s)
      Kang H, Kwon M J, Kim S, Lee S, Jones T G, Johncock A C, Haraguchi A, Freeman C
    • Journal Title

      Nature Communications

      Volume: 9 Issue: 1 Pages: 3807-3807

    • DOI

      10.1038/s41467-018-06259-1

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Inorganic nitrogen source for autotrophic growth of Euglena mutabilis Schmitz.2018

    • Author(s)
      Zheng J., Haraguchi A
    • Journal Title

      Phycological Research

      Volume: 66 Issue: 2 Pages: 155-158

    • DOI

      10.1111/pre.12211

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Concentration of Some Trace Elements in Two Wild Edible Ferns, Diplazium esculentum and Stenochlaena palutris, Inhabiting Tropical Peatlands under Different Environments in Central Kalimantan.2017

    • Author(s)
      Rahmawati D., Wijaya H. C., Hashidoko Y., Djajakirana G., Haraguchi A., Watanabe T., Kuramochi K., Nion Y. A.
    • Journal Title

      Eurasian Journal of Forest Research

      Volume: 20 Pages: 11-20

    • NAID

      120006370631

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Assessment of heavy metals in water, sediment, Anabas testudineus and Eichhornia crassipes in a former mining pond in Perak, Malaysia2017

    • Author(s)
      Kamari A., Yusof N., Abdullah H., Haraguchi A. Abas M. A.
    • Journal Title

      Chemistry and Ecology

      Volume: 33 Issue: 7 Pages: 637-651

    • DOI

      10.1080/02757540.2017.1351553

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Denial of long-term issues with agriculture on tropical peatlands will have devastating consequences2017

    • Author(s)
      Wijedasa, L. S., Jauhiainen, J., Ononen, M. K., Lampela, M., Vasander, H. et al.
    • Journal Title

      Global Change Biol.

      Volume: 23 Issue: 3 Pages: 977-982

    • DOI

      10.1111/gcb.13516

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] New Discussion on Boysen-Jensen's Photosynthetic Response Curves Under Plant Canopy and Proposal of Practical Equations for Monitoring and Management of Canopy Photosynthesis2016

    • Author(s)
      Yuta OKAMOTO, Akira HARAGUCHI, Takuya SUZUKI, Tomonori KAWANO
    • Journal Title

      Environment Control in Biology

      Volume: 54 Issue: 1 Pages: 7-16

    • DOI

      10.2525/ecb.54.7

    • NAID

      130005122795

    • ISSN
      1880-554X, 1883-0986
    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] 陸水圏の硫酸汚染問題に関する国際連携研究2016

    • Author(s)
      原口昭
    • Journal Title

      環境創

      Volume: 5 Pages: 18-19

    • Related Report
      2016 Research-status Report
  • [Presentation] Exact measurement of photosynthetic carbon accumulation rate in Sphagnum community.2016

    • Author(s)
      Akira Haraguchi
    • Organizer
      The 14th International Peat Congress
    • Place of Presentation
      Kuching, Sarawak, Malaysia
    • Year and Date
      2016-08-15
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research

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Published: 2016-04-21   Modified: 2020-03-30  

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