A model construction for ammonia assimilation process in conifer photorespiration
Project/Area Number |
16K07791
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Forest science
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Research Institution | Forest Research and Management Organization |
Principal Investigator |
Miyazawa Shin-Ichi 国立研究開発法人森林研究・整備機構, 森林総合研究所, 主任研究員 等 (10578438)
|
Project Period (FY) |
2016-04-01 – 2019-03-31
|
Project Status |
Completed (Fiscal Year 2018)
|
Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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Keywords | 光合成 / 光呼吸 / 針葉樹 / 被子植物 / アンモニア / 二酸化炭素 / グルタミン合成酵素 / 葉緑体型グルタミン合成酵素 / 葉緑体型グルタミン合成酵素の欠如 / 針葉樹の代謝特異性 / 植物 / 生理学 / 生態学 / 遺伝子 / 林学 |
Outline of Final Research Achievements |
Photosynthesis comprises a series of complex reactions, including the metabolic process known as “photorespiration” which produces ammonia. In the established theory, ammonia generated during photorespiration is immediately assimilated through catalysis by glutamine synthetase in chloroplasts (GS2) into an amino acid called glutamine. We tested for the presence or absence of GS2 in various conifer leaves, finding only the cytosolic glutamine synthetase isoform (GS1); GS2 was not detected. Furthermore, we found that leaves of conifers have very low assimilation efficiency of photorespiratory ammonia in comparison to leaves of crops and broadleaf trees.
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Academic Significance and Societal Importance of the Research Achievements |
植物の光合成は20種類以上の酵素からなる複雑な代謝反応です。本研究によって、針葉樹には光合成の一部である、”光呼吸”という代謝にかかわる重要な酵素が欠如していること、針葉樹と広葉樹とでは光呼吸の効率に違いがあることが明らかになりました。光合成のメカニズムは、針葉樹と広葉樹とで大きな違いはないと考えられてきましたが、今後、そのメカニズムの見直しが必要といえます。
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Report
(4 results)
Research Products
(7 results)