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A model construction for ammonia assimilation process in conifer photorespiration

Research Project

Project/Area Number 16K07791
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Forest science
Research InstitutionForest Research and Management Organization

Principal Investigator

Miyazawa Shin-Ichi  国立研究開発法人森林研究・整備機構, 森林総合研究所, 主任研究員 等 (10578438)

Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords光合成 / 光呼吸 / 針葉樹 / 被子植物 / アンモニア / 二酸化炭素 / グルタミン合成酵素 / 葉緑体型グルタミン合成酵素 / 葉緑体型グルタミン合成酵素の欠如 / 針葉樹の代謝特異性 / 植物 / 生理学 / 生態学 / 遺伝子 / 林学
Outline of Final Research Achievements

Photosynthesis comprises a series of complex reactions, including the metabolic process known as “photorespiration” which produces ammonia. In the established theory, ammonia generated during photorespiration is immediately assimilated through catalysis by glutamine synthetase in chloroplasts (GS2) into an amino acid called glutamine. We tested for the presence or absence of GS2 in various conifer leaves, finding only the cytosolic glutamine synthetase isoform (GS1); GS2 was not detected. Furthermore, we found that leaves of conifers have very low assimilation efficiency of photorespiratory ammonia in comparison to leaves of crops and broadleaf trees.

Academic Significance and Societal Importance of the Research Achievements

植物の光合成は20種類以上の酵素からなる複雑な代謝反応です。本研究によって、針葉樹には光合成の一部である、”光呼吸”という代謝にかかわる重要な酵素が欠如していること、針葉樹と広葉樹とでは光呼吸の効率に違いがあることが明らかになりました。光合成のメカニズムは、針葉樹と広葉樹とで大きな違いはないと考えられてきましたが、今後、そのメカニズムの見直しが必要といえます。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (7 results)

All 2019 2018 2017 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (4 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results) Remarks (2 results)

  • [Journal Article] Low assimilation efficiency of photorespiratory ammonia in conifer leaves2018

    • Author(s)
      Miyazawa Shin-Ichi、Nishiguchi Mitsuru、Futamura Norihiro、Yukawa Tomohisa、Miyao Mitsue、Maruyama Tsuyoshi Emilio、Kawahara Takayuki
    • Journal Title

      Journal of Plant Research

      Volume: 131 Issue: 5 Pages: 789-802

    • DOI

      10.1007/s10265-018-1049-2

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 草本植物との比較から見えてくる、樹木の光合成系における窒素利用の特異性2019

    • Author(s)
      宮澤真一
    • Organizer
      日本森林学会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Difference in assimilation efficiency of photorespiratory ammonia between angiosperm and gymnosperm leaves2018

    • Author(s)
      MIYAZAWA Shin-Ichi、IHARA Tokuko
    • Organizer
      First European Congress in Photosynthesis Research
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Low NH3 assimilation efficiency in conifer photorespiration: chloroplastic glutamine synthetase (GS2) as an adaptive tratit to low CO2 environments on earth(針葉樹の光呼吸における低いアンモニア同化効率:葉緑体型グルタミン合成酵素(GS2)の獲得は地球の低CO2環境に対する適応なのか?)2017

    • Author(s)
      MIYAZAWA Shin-Ichi(宮澤真一)、NISHIGUCHI Mitsuru(西口満)、FUTAMURA Norihiro(二村典宏・元森林総研)、YUKAWA Tomohisa(遊川知久・筑波実験植物園)、MIYAO Mitsue(宮尾光恵・東北大)、Maruyama E. Tsuyoshi(丸山毅)、KAWAHARA Takayuki(河原孝行)
    • Organizer
      グラハム・ファーカー教授2017年京都賞受賞記念ワークショップ(東京大学開催)
    • Related Report
      2017 Research-status Report
  • [Presentation] 裸子植物と被子植物の光呼吸経路は本当に同じなのか?2017

    • Author(s)
      宮澤真一、遊川知久、河原孝行
    • Organizer
      第64回日本生態学会
    • Place of Presentation
      早稲田大学(東京都新宿区)
    • Related Report
      2016 Research-status Report
  • [Remarks] アンモニアの研究から見えてきた針葉樹の光合成の謎

    • URL

      https://www.ffpri.affrc.go.jp/pubs/kikan/documents/kikanffpri-41.pdf

    • Related Report
      2018 Annual Research Report
  • [Remarks] 光合成の代謝系における針葉樹の特異性が明らかに

    • URL

      https://www.ffpri.affrc.go.jp/research/saizensen/2018/20180709-01.html

    • Related Report
      2018 Annual Research Report

URL: 

Published: 2016-04-21   Modified: 2020-03-30  

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