| Project/Area Number |
16K07814
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Wood science
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| Research Institution | Kanagawa Institute of Technology |
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Principal Investigator |
Nakagame Seiji 神奈川工科大学, 応用バイオ科学部, 准教授 (00605206)
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | イソプレン / リグノセルロース / 白色腐朽菌 / ヒラタケ / 非可食性バイオマス / バイオリファイナリー / バイオマス |
|---|
| Outline of Final Research Achievements |
The high degradability of lignocelluloses by white-rot fungi would be a beneficial for simplifying the isoprene producing process from lignocelluloses. In this study, the codon-optimized isoprene synthase gene for Pleurotus ostreatus was chemically synthesized based on the isoprene synthase gene from Pueraria montana (kudzu vine). The constructed plasmid for producing isoprene was introduced into P. ostreatus host strain. Five transformants harboring the codon-optimized isoprene synthase gene were selected and the productions of isoprene were compared with the host strain by GC-MS. It was shown that the transformants produced isoprene in the presence of lignocelluloses such as wood meal and crystalline cellulose, while the host did not produce isoprene. This result suggests that using isoprene producible white-rot fungi have a high potential to produce isoprene from lignocellulose.
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| Academic Significance and Societal Importance of the Research Achievements |
基幹化成品中間原料であるイソプレンを生産できる白色腐朽菌を分子育種により作製し、バイオマス資源を原料として用いたイソプレン製造方法を確立することで、合成ゴムの原料供給源の多様化に貢献するとともに、イソプレン製造時の化石資源の使用量を軽減させることで、地球温暖化の原因であるCO2排出量削減に寄与できる。
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