| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
We define an artificial fusion protein for complexing any protein "X" to paper via the hyperthermostable chitin/cellulose binding domain (hCBD) as "X"-C Link. We have originally developed BAF-C Link using BRET-based Auto-illuminated Fluorescent protein (BAF) that reproduces the green light emission mechanism of jellyfish GFP, and developed a unique technology to combine BAF on paper.
In this study, we developed anti-GFP VHH-C Link using a specific VHH antibody against GFP in order to newly add the value of antibody function to paper. The anti-GFP VHH/paper hybrid was able to capture EGFP even after dry storage for 1 year or more at room temperature, and its application to lateral flow devices was also possible.
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