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Studies on physiological functions of saccus vasculosus in Japanese eel

Research Project

Project/Area Number 16K07846
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Aquatic bioproduction science
Research InstitutionUniversity of Miyazaki

Principal Investigator

Kagawa Hirohiko  宮崎大学, 農学部, 教授 (60169381)

Co-Investigator(Kenkyū-buntansha) 内田 勝久  宮崎大学, 農学部, 教授 (50360508)
Research Collaborator MIYANISHI hiroshi  
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsニホンウナギ / 血嚢体 / 光受容 / 成熟制御機構 / 成熟誘導技術 / シラスウナギ / 種苗生産 / 性成熟誘導 / 性成熟 / 成熟誘導 / 光受容体 / 光周性 / 催熟誘導 / 水産学 / 生理学
Outline of Final Research Achievements

The physiological function of Japanese eel saccus vasculosus (SV) has long been unknown. In this study, to clarify the photoperiodic and the physiological roles of SV in the Japanese eel, we examined the presence of rhodopsin family genes and key genes regulating seasonal reproduction in the SV. By means of real time quantitative PCR, we found that photoreceptors, such as short wavelength-sensitive opsin 2 (sws2, blue sensitive) was highly expressed in the SV and increased from summer to winter in male eels reared in the outside pond. Moreover, we found that iodothyronine 5'-deiodinase (dio2), tshβ, and gpα expressed in the SV and these factors also increased during the process of puberty. We also demonstrated that removal of SV by surgical surgery decreased gene expression of these factors and depressed the development of oocytes. These results indicate that saccus vasculosus has important physiological function of photoreceptor and the sexual maturation in Japanese eel.

Academic Significance and Societal Importance of the Research Achievements

シラスウナギの大量種苗生産技術を開発するためには安定的に簡便な方法で受精卵を確保する必要がある。これまで、受精卵を得るためにはホルモン注射により成熟を促進し、人工授精等により受精卵を確保していた。この手法は煩雑で受精卵の質もあまり良くない。本研究では、光や水温などの環境因子により成熟を促進する技術を開発することを目指して、これまで不明であった血嚢体の生理機能を明らかにすることを目的として実験を行い、脳深部にある血脳体が青色光を受容する光受容器官であり、初期成熟に重要な役割を果たすことを明らかにした。この情報をもとに、環境因子による効率的な種苗生産技術が開発されることが期待される。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (6 results)

All 2017 2016 Other

All Int'l Joint Research (1 results) Presentation (5 results) (of which Int'l Joint Research: 3 results)

  • [Int'l Joint Research] Korea Institute of Ocean Science/Solforto Co. Ltd(韓国)

    • Related Report
      2016 Research-status Report
  • [Presentation] ニホンウナギ血嚢体の光受容器官としての生理機能の解明2017

    • Author(s)
      宗石 瞬 、Lim Bongsoo 、Sungpyo Hur、 内田勝久、香川浩彦(宮崎大農)
    • Organizer
      日本水産学会春季大会
    • Place of Presentation
      東京海洋大学
    • Year and Date
      2017-03-26
    • Related Report
      2016 Research-status Report
  • [Presentation] Elucidation of physiological functions of saccus vasculosus as a photoperiodic organ in Japanese eel (Anguilla japonica )2017

    • Author(s)
      Shun Muneishi, Lim Bongsoo, Sungpyo Hur, Katsuhisa Uchida and Hirohiko Kagawa
    • Organizer
      The JSFS 85th Anniversary-Commemorative International Symposium “Fisheries Science for Future Generations”
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Elucidation of physiological functions as a photoreceptor organ of saccus vasculosus in Japanese eel (Anguilla japonica)2017

    • Author(s)
      Shun Muneishi, Lim Bongsoo, Sungpyo Hur, Katsuhisa Uchida, Hirohiko Kagawa
    • Organizer
      1st Joint Symposium on Aquaculture Science between University of Miyazaki, Japan and National Institute of Fisheries Science, Korea 2017
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Studies on physiology of eel in University of Miyazaki2017

    • Author(s)
      Hirohiko Kagawa
    • Organizer
      Workshop on Establishment of East Asia Conservation network for sustainable fish resources
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Saccus vasculosus is a photoperiodic sensory and endocrine organ in Japanese eel reproduction2016

    • Author(s)
      Misa Hamada, Katsuhisa Uchida, Fumiya Furukawa, Yuta Seko, Hirohiko Kagawa
    • Organizer
      The 8th International Symposium on Fish Endocrinology
    • Place of Presentation
      Gothenburg, Sweden
    • Year and Date
      2016-06-28
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2022-02-16  

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