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Evaluation of risks of genetic pollution in the natural Pen shell resources

Research Project

Project/Area Number 16K07859
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Aquatic bioproduction science
Research InstitutionFisheries Research and Education Agency

Principal Investigator

Sekino Masashi  国立研究開発法人水産研究・教育機構, 中央水産研究所, グループ長 (90371799)

Co-Investigator(Kenkyū-buntansha) 藤原 篤志  国立研究開発法人水産研究・教育機構, 中央水産研究所, 主幹研究員 (30443352)
橋本 和正  国立研究開発法人水産研究・教育機構, 西海区水産研究所, 主任研究員 (40372007)
佐々木 猛智  東京大学, 総合研究博物館, 准教授 (70313195)
山田 勝雅  熊本大学, くまもと水循環・減災研究教育センター, 特任助教 (80569195)
Project Period (FY) 2016-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords交雑 / 遺伝子浸透 / 集団遺伝学 / 集団ゲノム学 / 一塩基多型 / 遺伝子汚染 / 遺伝子移入 / 集団ゲノミクス / タイラギ / 二枚貝 / 保全遺伝学 / 分子生態学 / 遺伝的多様性保全 / RAD / 分類学 / ゲノム / 遺伝学
Outline of Final Research Achievements

The pen shell Atrina pectinata sensu lato distributed in Japan is classified into two forms according to the shell morphs, i.e., scaly (SC) and non-scaly (NS). The two forms are thought to be different species; however, individuals with ambiguous and/or intermediate shell morph have frequently been found in Seto Inland Sea, where both SC and NS occur in sympatry. We consider that such ambiguous shells are derived from hybridization/introgression between the two species. This issue, however, has not been studied in detail heretofore. Thus, we assessed the possible occurrence of hybridization/introgression between SC and NS based on single nucleotide polymorphisms. Our results evidenced ongoing hybridization/introgression in Stouchi Inland Sea, as F1s, F2s, and bi-directional backcrosses were detected there.

Academic Significance and Societal Importance of the Research Achievements

本課題により、瀬戸内海産タイラギの有鱗型と無鱗型間で、交雑と双方向の遺伝子浸透(雑種を介した一方の種から他方の種への遺伝子移入)が生じていることが示された。過去のアイソザイム研究では、両者の雑種第一世代F1が生じていることは推測されていたが、本課題では、さらにF2(F1間の子供)や、両種への戻し交配個体(F1-純種間の子供)も生じていることが明らかになった。これらの結果は、タイラギ類の分類学、進化生物学および分子生態学上の学術的価値が高いだけではなく、種苗生産・放流によるタイラギ増殖の遺伝的攪乱リスクの啓蒙と資源保全・増殖戦略の策定のための重要な知見となる。

Report

(5 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (19 results)

All 2019 2018 2017 2016

All Journal Article (7 results) (of which Peer Reviewed: 6 results,  Open Access: 2 results) Presentation (12 results) (of which Int'l Joint Research: 3 results)

  • [Journal Article] Morphological variations among distinct genotypes of the pen shell Atrina pectinata in Japan and Korea2019

    • Author(s)
      Yamada K, Hashimoto K, Sasaki T, Kobayashi M, Henmi Y, Sekino M
    • Journal Title

      Proceedings of the 2019 CWMD Conference

      Volume: 2019 Pages: 808-813

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Lineage specific detection of the scaly form of the pen shell Atrina spp. by a loop-mediated isothermal amplification method2018

    • Author(s)
      Hashimoto K, Yamada K, Nagae A, Matsuyama Y
    • Journal Title

      Fisheries Science

      Volume: 84 Issue: 5 Pages: 837-848

    • DOI

      10.1007/s12562-018-1231-4

    • NAID

      40021650855

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Genotyping-by-sequencing for construction of a new genetic linkage map and QTL analysis of growth-related traits in Pacific bluefin tuna2018

    • Author(s)
      Uchino T, Hosoda E, Nakamura Y, Yasuike M, Mekuchi M, Sekino M, Fujiwara A, Sugaya T, Tanaka Y, Kumon K, Agawa Y, Sawada Y, Sano M, Sakamoto T
    • Journal Title

      Aquaculture Research

      Volume: 49 Issue: 3 Pages: 1293-1301

    • DOI

      10.1111/are.13584

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Survival time of juvenile pen shell Atrina pectinata (Bivalvia: Pinnidae) in hyposaline water2018

    • Author(s)
      Kurihara T, Nakano S, Matsuyama Y, Hashimoto K, Yamada K, Ito A, Kanematsu M
    • Journal Title

      International Aquatic Research

      Volume: 10 Issue: 1 Pages: 1-11

    • DOI

      10.1007/s40071-017-0183-0

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] DNA多型でみるタイラギ二種の違い2017

    • Author(s)
      關野正志・藤原篤志・橋本和正・山田勝雅・佐々木猛智
    • Journal Title

      平成28年度中央水産研究所主要成果集「研究のうごき」

      Volume: 15 Pages: 13-13

    • Related Report
      2017 Research-status Report
  • [Journal Article] DNA親子判別における一塩基多型(SNPs)の 有効性の検証2017

    • Author(s)
      關野正志, 中道礼一郎・藤原篤志・西木一生・松原和純・岩崎裕貴・鈴木伸明
    • Journal Title

      水産育種

      Volume: 46 Pages: 79-85

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] A new resource of single nucleotide polymorphisms in the Japanese eel Anguilla japonica derived from restriction site-associated DNA2016

    • Author(s)
      Masashi Sekino, Reiichiro Nakamichi, Yuki Iwasaki(他5名)
    • Journal Title

      Ichthyological Research

      Volume: 63 Issue: 4 Pages: 496-504

    • DOI

      10.1007/s10228-016-0518-7

    • NAID

      40020979345

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Presentation] Morphological variations among distinct genotypes of the pen shell Atrina pectinata in Japan and East Asia2019

    • Author(s)
      Yamada K, Hashimoto K, Sasaki T, Kobayashi M, Sekino M
    • Organizer
      2019 International Conference on Climate Change, Disaster Management and Environmental Sustainability
    • Related Report
      2019 Annual Research Report
  • [Presentation] 香川県海域で初めて記録されたLineage6タイラギ2019

    • Author(s)
      橋本和正、關野正志、小林真弓、山本昌幸、山田勝雅、逸見泰久、佐々木猛智
    • Organizer
      令和元年度 日本水産学会秋季大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 有明海におけるタイラギの移植適水域2018

    • Author(s)
      栗原健夫, 松山幸彦, 橋本和正, 圦本達也, 長副聡, 岡村和麿, 中野昌次, 速水祐一, 山口創一
    • Organizer
      2018年日本プランクトン学会・ベントス学会合同大会
    • Related Report
      2018 Research-status Report
  • [Presentation] 貧酸素水塊の移入に対するベントス群集の応答:群集形成パターンの評価2018

    • Author(s)
      4)山田勝雅, 小森田智大, 宮本 康, 石松将武, Wachirah Jaingam, 堤 裕昭, 逸見泰久
    • Organizer
      日本プランクトン学会・日本ベントス学会合同大会
    • Related Report
      2018 Research-status Report
  • [Presentation] 汽水域の漁獲ベントスの時空間動態からメタ個体群動態を推定する2017

    • Author(s)
      山田勝雅
    • Organizer
      第130回汽水域懇談会
    • Related Report
      2017 Research-status Report
  • [Presentation] Community structure of benthic infauna in relation to spatial scale resolutions at Midorikawa-River tidal flats of Ariake Bay, Kyusyu, Japan: focusing on nested community structure.2017

    • Author(s)
      Yamada K, Komorita T, Takenaka R, Morokuma T, Kuroki Y, Uchikawa J
    • Organizer
      The 3rd Asian Marine Biology Symposium
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] RAD-SNPに基づく有鱗型・無鱗型タイラギ間の遺伝的差異2017

    • Author(s)
      関野正志・藤原篤志・中村洋路(他9名)
    • Organizer
      H29度日本水産学会春季大会
    • Place of Presentation
      東京
    • Related Report
      2016 Research-status Report
  • [Presentation] タイラギ外套膜から綿棒で採取できるDNA量の推定2017

    • Author(s)
      橋本和正・中野昌次・山田勝雅(他2名)
    • Organizer
      H29度日本水産学会春季大会
    • Place of Presentation
      東京
    • Related Report
      2016 Research-status Report
  • [Presentation] 状態空間モデルによる個体群動態の推定:有明海の二枚貝資源量はなぜ減少したのか?2017

    • Author(s)
      山田勝雅, 長田穣, 中野善, 岡村和麿
    • Organizer
      第64回日本生態学会
    • Place of Presentation
      東京
    • Related Report
      2016 Research-status Report
  • [Presentation] タイラギ親貝の非破壊的遺伝子型判別手法の開発2017

    • Author(s)
      橋本和正・永江 彬・中野昌次・山田勝雅・松山幸彦
    • Organizer
      H29度日本水産学会春季大会
    • Place of Presentation
      東京
    • Related Report
      2016 Research-status Report
  • [Presentation] Genotype identification of the pen shell Atrina pectinata by using LAMP method2016

    • Author(s)
      Kazumasa Hashimoto, Katsumasa Yamada, Masashi Sekino, Takenori Sasaki(他4名)
    • Organizer
      The 7th World Fisheries Congress
    • Place of Presentation
      韓国、釜山
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Shell morphs-variations among distinct genotypes of the pen shell Atrina pectinata2016

    • Author(s)
      Katsumasa Yamada, Kazumasa Hashimoto, Masashi Sekino, Takenori Sasaki
    • Organizer
      The 7th World Fisheries Congress
    • Place of Presentation
      韓国、釜山
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research

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Published: 2016-04-21   Modified: 2021-02-19  

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