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Molecular mechanism of neutralization of acid envirnments by alkalinization yeasts and their enzymes

Research Project

Project/Area Number 16K07868
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Aquatic life science
Research InstitutionTokyo University of Marine Science and Technology

Principal Investigator

Urano Naoto  東京海洋大学, 学術研究院, 教授 (90262336)

Co-Investigator(Kenkyū-buntansha) 岡井 公彦  東京海洋大学, 学術研究院, 助教 (00596562)
石田 真巳  東京海洋大学, 学術研究院, 教授 (80223006)
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords酵母 / 強酸性水 / 中和 / pH耐性 / 分子機構 / 酵素 / 酸性水圏 / 中性水圏 / 塩基性水圏 / アルカリ化酵母 / 広域pH耐性酵母 / バイオリアクター / アミノ酸代謝 / 強酸性水圏 / 中性圏 / 中和活性 / 中和能 / 生態系 / 微生物 / 環境 / 遺伝子 / バイオテクノロジー
Outline of Final Research Achievements

Alkalinization yeasts have neutralizing activities of strongly acidified water by producing anmonium ions. The yeasts were cultivated in acid, neutral, or basic water and their protein biosynthesis in the cells changed little. The yeasts produced anmonium ions in each water. Production of organic acids by the yeasts increased in both neutral and basic water. Therefore, they were found to regulate pH by changing the pruductions of anmonium ions and organic acids accoding to environmental pH.
Alkalinization yeasts were found to live in natural acid, neutral or basic environments. The yeast species were almost same, had broad range of pH tolerance, and lived in the environments from acid to basic.
Continous neutralization of strongly acidified water and removal of surplus anmonium ions were attained by a bioreactor with immobilized yeasts and zeolite.

Academic Significance and Societal Importance of the Research Achievements

日本にはpH1-3の強酸性の第1級河川が幾つか存在する。こうした河川では魚介類が生息せず、農業用水や養殖水にも使用できない死の川と言われてきた。石灰投与による中和事業が進み、中和工場に下流からは、河川に中和水が流れるようになった。一方で石灰による2次汚染も発生して、あるレベルでの石灰耐性を持つ魚介類しか生息できない水圏環境となっている。
アルカリ化酵母は、アンモニウムイオンを生成することで強酸性水を中和する能力を持つ。当該酵母により強酸性水を中和して、過剰な案もアンモニウムイオンを吸着除去することで、環境に優しい中和が実現できる。本研究成果は生活水や農業用水の製造に利用可能と考えている。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (11 results)

All 2019 2018 2017 2016

All Journal Article (7 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 5 results,  Open Access: 6 results) Presentation (4 results)

  • [Journal Article] Effect of Acid Species on Cryptococcus sp. T1 and Construction of Its Bioreactor System for Continuous Neutralization of Acidic Media2019

    • Author(s)
      Shintato Nagaoka, Yuko Kajiwara, Sayaka Toyama, Masahiko Okai, Masami Ishida, Naoto Urano
    • Journal Title

      Advances in Microbiology

      Volume: 9 Issue: 01 Pages: 74-86

    • DOI

      10.4236/aim.2019.91006

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Molecular Phylogeny and Phenotypic Characterization of Yeasts with a Broad Range of pH Tolerance Isolated from Natural Aquatic Environments2019

    • Author(s)
      Naoto Urano, Ayaka Shirao, Yuka Naito, Masahiko Okai, Masami Ishida, Masachika Takashio
    • Journal Title

      Adavances in Microbiology

      Volume: 9 Issue: 01 Pages: 56-73

    • DOI

      10.4236/aim.2019.91005

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 強酸性環境を中和する酵母2018

    • Author(s)
      浦野直人、長岡真太郎、岡井公彦
    • Journal Title

      科学・技術研究

      Volume: 7 Pages: 27-33

    • NAID

      130007402925

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Characterization of yeasts capable of neuralizing acidic media from natural neutral environments2018

    • Author(s)
      Shintaro Nagaoka, Takako Kobayashi, Yuko Kajiwara, Masahiko Okai, Masami Ishida, Naoto Urano
    • Journal Title

      Advances in Microbiology

      Volume: 7 Pages: 887-897

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Isolation of aquatic yeasts with the ability to neutralize acidic media, from an extremely acidic river near Japan's Kusatsu-Shirane Volacano2017

    • Author(s)
      Daisuke Mitsuya, Takuya Hayashi, Yu Wang, Mami Tanaka, Masahiko Okai, Masami Ishida, and Naoto Urano
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 124(1) Pages: 43-46

    • Related Report
      2017 Research-status Report 2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Neutralization of acidic drainage by Cryputococcous sp. by immobilied in alginate beads2017

    • Author(s)
      Masahiko Okai, Chisato Suwa, Shintaro Nagaoka, Nobuo Obara, Masami, Ishida, Naoto Urano
    • Journal Title

      Bioscience, Bioctechnology, and Biochemistry

      Volume: 81(11) Pages: 2216-2224

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 水圏に生息する天然酵母の単離と有効利用2016

    • Author(s)
      浦野直人、三矢太亮、岡井公彦
    • Journal Title

      生物工学会誌

      Volume: 第6号 Pages: 319-323

    • Related Report
      2016 Research-status Report
    • Open Access
  • [Presentation] 強酸性水圏由来微生物に関する研究11:アルカリ化酵母による酸性水中和の分子機構解析2018

    • Author(s)
      長岡真太郎、梶原優子、岡井公彦、石田真巳、浦野直人
    • Organizer
      平30年度日本水産学会春期大会
    • Related Report
      2017 Research-status Report
  • [Presentation] 強酸性水圏由来微生物に関する研究12:アルカリ化酵母による強酸性水中和システムに及ぼすアルミニウムイオンの影響2018

    • Author(s)
      梶原優子、長岡真太郎、岡井公彦、石田真巳、浦野直人
    • Organizer
      平成30年度日本水産学会春期大会
    • Related Report
      2017 Research-status Report
  • [Presentation] 強酸性水圏由来微生物に関する研究9:アルカリ化酵母・酵素による酸性水中和機構の解明2016

    • Author(s)
      長岡真太郎、梶原優子、岡井公彦、石田真巳、浦野直人
    • Organizer
      平成28年度日本水産学会秋季大会
    • Place of Presentation
      近畿大学農学部奈良キャンパス
    • Related Report
      2016 Research-status Report
  • [Presentation] 強酸性水圏由来微生物に関する研究10:アルカリ化酵母・酵素バイオリアクターによる強酸性水の中和システムも構築2016

    • Author(s)
      梶原優子、長岡真太郎、岡井公彦、石田真巳、浦野直人
    • Organizer
      平成28年度日本水産学会秋季大会
    • Place of Presentation
      近畿大学農学部奈良キャンパス
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2020-03-30  

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