Improvement of donor germ cells functions by surrogate host
| Project/Area Number |
16K07874
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Aquatic life science
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| Research Institution | Ehime University |
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Principal Investigator |
Saito Taiju 愛媛大学, 南予水産研究センター, 准教授(特定教員) (90396309)
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 借腹生産 / 代理親生産 / 始原生殖細胞 / 移植 / PGCs / 生殖系列キメラ / 代理親効果 / ゼブラフィッシュ |
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| Outline of Final Research Achievements |
"Surrogate production" is a technique to achieve efficient production of target gametes via surrogate parents by inducing germ line chimera. The germ line chimera can be produced by transplanting germ line stem cells, such as primordial germ cells (PGCs), spermatogoina, and oogonia. In this study, we test if there are "parental effects" on germ cells by using different zebrafish strains, Casper and AB. We transplanted Casper PGCs into AB blastula embryos with BT-method to produce germ line chimeras. The host embryos were injected with MO against dnd gene in advance to remove endogenous PGCs. As a result, the survival rate of Casper larvae obtained from germ line chimeras was significantly higher than that of normal Casper strain. In addition to this, their chorion were thicker than controls, and CASA analysis revealed sperm from chimeras showed better scores than controls.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究では、代理親を経由してドナー配偶子生産を行うことにより、魚類配偶子の一部機能（卵膜の厚み、受精卵の発生率、精子活性等）が高まることが明らかとなった。すなわち、本研究を応用することにより、遺伝子の改変を伴うことなく、代理親生産技術により目的系統（種）の配偶子機能を高めることができる可能性がある。より妊性が高く、より卵質の高い配偶室を形成できる宿主系統を確立すれば、あらゆる系統の配偶子を高品質に生産できる可能性がある。
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Report
(4 results)
Research Products
(22 results)
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[Journal Article] Early embryonic development in pikeperch (Sander lucioperca) related to micromanipulation.2016
Author(s)
Guralp, H., Pocherniaieva, K., Blecha, M., Policar, T., Psenicka, M., Saito, T.
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Journal Title
Czech Journal of Animal Science
Volume: 61
Issue: 6
Pages: 273-280
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] The migration of primordial germ cells during late embryogenesis of pikeperch Sander lucioperca relative to blastomere transplantation2016
Author(s)
Guralp, H., Pocherniaieva, K., Blecha, M., Policar, T., Psenicka, M., Saito, T.
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Journal Title
Czech Journal of Animal Science
Volume: 62
Issue: 3
Pages: 121-129
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Presentation] Generation of Germline Chimera in Sturgeon.2017
Author(s)
Psenicka, M., Guralp, H., Pocherniaieva, K., Linhartova, Z., Iegorova, V., Saito, T.
Organizer
6th International Workshop on the Biology of Fish Gametes
Related Report
Int'l Joint Research
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[Presentation] Expression Profiling of New Germline Markers Suggests Successive Waves of Different Spermatogonial Stem Cell Subpopulations During Rainbow Trout Ontogenesis.2017
Author(s)
Maouche, A., Curran, E., Gautier, A., Goupil, AS., Sambroni, E., Dupin de Breyssat, E., Guiguen, Y., Saito, T., Le Gac, F., Lareyre, JJ.
Organizer
6th International Workshop on the Biology of Fish Gametes
Related Report
Int'l Joint Research
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[Presentation] Triploid or Hybrid Fish: Which is the Best Host for Stem Germ Cells?2017
Author(s)
Siqueira-Silva, DH., Piva, LH., Goes, CAG., Fujimoto, T., Saito, T., Dragone, LV., Senhorini, JA., Porto-Foresti, F., Ferraz, JBS., Yasui, GS.
Organizer
6th International Workshop on the Biology of Fish Gametes
Related Report
Int'l Joint Research
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