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Improvement of donor germ cells functions by surrogate host

Research Project

Project/Area Number 16K07874
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Aquatic life science
Research InstitutionEhime University

Principal Investigator

Saito Taiju  愛媛大学, 南予水産研究センター, 准教授(特定教員) (90396309)

Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords借腹生産 / 代理親生産 / 始原生殖細胞 / 移植 / PGCs / 生殖系列キメラ / 代理親効果 / ゼブラフィッシュ
Outline of Final Research Achievements

"Surrogate production" is a technique to achieve efficient production of target gametes via surrogate parents by inducing germ line chimera. The germ line chimera can be produced by transplanting germ line stem cells, such as primordial germ cells (PGCs), spermatogoina, and oogonia. In this study, we test if there are "parental effects" on germ cells by using different zebrafish strains, Casper and AB. We transplanted Casper PGCs into AB blastula embryos with BT-method to produce germ line chimeras. The host embryos were injected with MO against dnd gene in advance to remove endogenous PGCs. As a result, the survival rate of Casper larvae obtained from germ line chimeras was significantly higher than that of normal Casper strain. In addition to this, their chorion were thicker than controls, and CASA analysis revealed sperm from chimeras showed better scores than controls.

Academic Significance and Societal Importance of the Research Achievements

本研究では、代理親を経由してドナー配偶子生産を行うことにより、魚類配偶子の一部機能(卵膜の厚み、受精卵の発生率、精子活性等)が高まることが明らかとなった。すなわち、本研究を応用することにより、遺伝子の改変を伴うことなく、代理親生産技術により目的系統(種)の配偶子機能を高めることができる可能性がある。より妊性が高く、より卵質の高い配偶室を形成できる宿主系統を確立すれば、あらゆる系統の配偶子を高品質に生産できる可能性がある。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (22 results)

All 2019 2018 2017 2016 Other

All Int'l Joint Research (4 results) Journal Article (7 results) (of which Int'l Joint Research: 7 results,  Peer Reviewed: 7 results,  Open Access: 5 results) Presentation (7 results) (of which Int'l Joint Research: 7 results,  Invited: 2 results) Book (3 results) Remarks (1 results)

  • [Int'l Joint Research] University of South Bohemia(チェコ)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] University of South Bohemia(チェコ)

    • Related Report
      2017 Research-status Report
  • [Int'l Joint Research] INRA(フランス)

    • Related Report
      2017 Research-status Report
  • [Int'l Joint Research] Monash University(オーストラリア)

    • Related Report
      2017 Research-status Report
  • [Journal Article] First evidence of viable progeny from three interspecific parents in sturgeon2018

    • Author(s)
      Iegorova Viktoriia、Havelka Milos、Psenicka Martin、Saito Taiju
    • Journal Title

      Fish Physiology and Biochemistry

      Volume: 44 Issue: 6 Pages: 1541-1550

    • DOI

      10.1007/s10695-018-0553-6

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Polyspermy produces viable haploid/diploid mosaics in sturgeon†2018

    • Author(s)
      Iegorova Viktoriia、Psenicka Martin、Lebeda Ievgen、Rodina Marek、Saito Taiju
    • Journal Title

      Biology of Reproduction

      Volume: 99 Pages: 695-706

    • DOI

      10.1093/biolre/ioy092

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Biotechnology applied to fish reproduction: tools for conservation2018

    • Author(s)
      Diogenes Henrique de Siqueira-Silva, Taiju Saito, Amanda Pereira dos Santos-Silva, Raphael da Silva Costa, Martin Psenicka, George Shigueki Yasui
    • Journal Title

      Fish Physiology and Biochemistry

      Volume: 44 Issue: 6 Pages: 1469-1485

    • DOI

      10.1007/s10695-018-0506-0

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Triploid or hybrid tetra: Which is the ideal sterile host for surrogate technology?2018

    • Author(s)
      Piva Lucas Henrique、de Siqueira-Silva Di?genes Henrique、Goes Caio Augusto Gomes、Fujimoto Takafumi、Saito Taiju、Dragone Let?cia Veroni、Senhorini Jos? Augusto、Porto-Foresti Fabio、Ferraz Jos? Bento Sterman、Yasui George Shigueki
    • Journal Title

      Theriogenology

      Volume: 108 Pages: 239-244

    • DOI

      10.1016/j.theriogenology.2017.12.013

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Development, and effect of water temperature on development rate, of pikeperch Sander lucioperca embryos2017

    • Author(s)
      Guralp H.、Pocherniaieva K.、Blecha M.、Policar T.、Psenicka M.、Saito T.
    • Journal Title

      Theriogenology

      Volume: 104 Pages: 94-104

    • DOI

      10.1016/j.theriogenology.2017.07.050

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Early embryonic development in pikeperch (Sander lucioperca) related to micromanipulation.2016

    • Author(s)
      Guralp, H., Pocherniaieva, K., Blecha, M., Policar, T., Psenicka, M., Saito, T.
    • Journal Title

      Czech Journal of Animal Science

      Volume: 61 Issue: 6 Pages: 273-280

    • DOI

      10.17221/35/2015-cjas

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The migration of primordial germ cells during late embryogenesis of pikeperch Sander lucioperca relative to blastomere transplantation2016

    • Author(s)
      Guralp, H., Pocherniaieva, K., Blecha, M., Policar, T., Psenicka, M., Saito, T.
    • Journal Title

      Czech Journal of Animal Science

      Volume: 62 Issue: 3 Pages: 121-129

    • DOI

      10.17221/40/2016-cjas

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Surrogate production in fish2018

    • Author(s)
      Taiju Saito
    • Organizer
      Workshop on Aquatic Sciences Biotechnology
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Generation of Germline Chimera in Sturgeon.2017

    • Author(s)
      Psenicka, M., Guralp, H., Pocherniaieva, K., Linhartova, Z., Iegorova, V., Saito, T.
    • Organizer
      6th International Workshop on the Biology of Fish Gametes
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Methods of Sperm Micro-Injection in Unfertilized Eggs: Could Be Used as Assisted Reproduction Techniques in Sturgeons?2017

    • Author(s)
      Fatira, E., Psenicka, M., Saito, T.
    • Organizer
      6th International Workshop on the Biology of Fish Gametes
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Expression Profiling of New Germline Markers Suggests Successive Waves of Different Spermatogonial Stem Cell Subpopulations During Rainbow Trout Ontogenesis.2017

    • Author(s)
      Maouche, A., Curran, E., Gautier, A., Goupil, AS., Sambroni, E., Dupin de Breyssat, E., Guiguen, Y., Saito, T., Le Gac, F., Lareyre, JJ.
    • Organizer
      6th International Workshop on the Biology of Fish Gametes
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Triploid or Hybrid Fish: Which is the Best Host for Stem Germ Cells?2017

    • Author(s)
      Siqueira-Silva, DH., Piva, LH., Goes, CAG., Fujimoto, T., Saito, T., Dragone, LV., Senhorini, JA., Porto-Foresti, F., Ferraz, JBS., Yasui, GS.
    • Organizer
      6th International Workshop on the Biology of Fish Gametes
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Development of Priomordial Germ Cells and Pigment Cells in Eastern Little Tuna, Euthynnus affinis, Embryos.2017

    • Author(s)
      Saito, T., Goto, R., Matsubara, T.
    • Organizer
      6th International Workshop on the Biology of Fish Gametes
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Sturgeon Phenomenon: First Evidence of Progeny from Three Parents.2017

    • Author(s)
      Iegorova, V., Psenicka, M., Saito T.
    • Organizer
      6th International Workshop on the Biology of Fish Gametes
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Book] Methods Mol Biol.2019

    • Author(s)
      Saito Taiju、Goto Rie、Rivers Nicola、Yamaha Etsuro
    • Total Pages
      434
    • Publisher
      Springer
    • ISBN
      9781493990085
    • Related Report
      2018 Annual Research Report
  • [Book] Methods Mol Biol.2018

    • Author(s)
      Goto Rie、Saito Taiju、Matsubara Takahiro、Yamaha Etsuro
    • Total Pages
      540
    • Publisher
      Springer
    • ISBN
      9781493988303
    • Related Report
      2018 Annual Research Report
  • [Book] ラボ必携 フローサイトメトリーQ&A2017

    • Author(s)
      戸村 道夫
    • Total Pages
      312
    • Publisher
      羊土社
    • ISBN
      9784758122351
    • Related Report
      2017 Research-status Report
  • [Remarks] 愛媛大学南予水産研究センター

    • URL

      http://ccr.ehime-u.ac.jp/cnf/

    • Related Report
      2018 Annual Research Report

URL: 

Published: 2016-04-21   Modified: 2022-02-22  

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