Molecular basis for onset of the novel 3rd toxicity of cyanotoxin microcystin-LR
| Project/Area Number |
16K07875
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Aquatic life science
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| Research Institution | Kagoshima University |
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Principal Investigator |
Komatsu Masaharu 鹿児島大学, 農水産獣医学域水産学系, 教授 (30325815)
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| Research Collaborator |
Takumi Shota
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | マイクロシスチン / OATP1B3 / アノイキス抵抗性 / 上皮・間葉転換 / 間葉・上皮転換 / マイクロシスチンLR / ノジュラリン / OATP1B1 / ファロイジン / 生薬 / アクテオシド / ハイポキシア / アノイキス / 毒性学 / 生理活性 / 衛生 / シグナル伝達 / 水産学 |
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| Outline of Final Research Achievements |
HEK293-OATP1B3FL (FL cells) and HEK293-OATP1B3AD (AD cells), which were transfomed cells after the parental HK293-OATP1B3 cells were exposed to MC-LR, were resistant to hypoxia than their parental cells.
Attenuation activity to the MC-LR cytotoxicity was detected in herbal medicines Iwajisha and Kin-oshi. In addition, we found that their components, acteoside, quercetin, and kaempferol were the active substances.
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| Academic Significance and Societal Importance of the Research Achievements |
アオコに由来する肝臓毒マイクロシスチンLRは，高濃度で肝機能不全を誘発し，低濃度慢性曝露により肝臓がんの発がん物質として作用する。本研究により，マイクロシスチンLRに肝がんの浸潤・転移を誘発する可能性があることを実験室レベルで示した。また，マイクロシスチンLRの細胞毒性を抑制する化合物を生薬中に見出した。発見した物質は野菜や果物にも含まれる成分であり，生薬や食事によりマイクロシスチンLR中毒を予防でき，また，発がんおよびがんの転移を抑える可能性が示唆された。
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Report
(4 results)
Research Products
(27 results)
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[Journal Article] NEU3 inhibitory effect of naringin suppresses cancer cell growth by attenuation of EGFR signaling through GM3 ganglioside accumulation.2016
Author(s)
Ayana Yoshinaga, Natsuki Kajiya, Kazuki Oishi, Yuko Kamada, Asami Ikeda, Petros Kingstone Chigwechokha, Toshiro Kibe, Michiko Kishida, Shosei Kishida, Masaharu Komatsu, Kazuhiro Shiozaki
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Journal Title
European Journal of Pharmacology
Volume: 782
Pages: 21-29
DOI
Related Report
Peer Reviewed
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[Journal Article] Positive regulation of myoblast differentiation by medaka Neu3b sialidase through ganglioside desialylation2016
Author(s)
Shiozaki, K., Harasaki, Y., Fukuda, M., Yoshinaga, A., Ryuzono, S., Chigwechokha, P., Komatsu M., Miyagi, T.
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Journal Title
Biochimie
Volume: 123
Pages: 65-72
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Eel green fluorescent protein is associated with resistance to oxidative stress.2016
Author(s)
Aki Funahashi, Masaharu Komatsu, Tatsuhiko Furukawa, Yuki Yoshizono, Hikari Yoshizono, Yasuhiro Orikawa, Shota Takumi, Kazuhiro Shiozaki, Seiichi Hayashi, Yoshio Kaminishi, Takao Itakura.
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Journal Title
Comparative Biochemistry and Physiology-part C
Volume: 181-182
Pages: 35-39
DOI
Related Report
Peer Reviewed
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[Journal Article] Lysosomal localization of Japanese medaka (Oryzias latipes) Neu1 sialidase and its highly conserved enzymatic profiles with human.2016
Author(s)
Ryuzono, S., Takase, R., Oishi, K., Ikeda, A., Chigwechokha, P., Funahashi, A., Komatsu, M., Miyagi, T., Shiozaki, K.,
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Journal Title
Gene
Volume: 575
Issue: 2
Pages: 513-523
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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