Development of strain and utilization of new and old world quail species

• Project/Area Number: 16K07986
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Animal production science
• Research Institution: Shinshu University
• Principal Investigator: Ono Tamao 信州大学, 学術研究院農学系, 教授 (10177264)
• Project Period (FY): 2016-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: コリンウズラ / ニホンウズラ / 遺伝資源 / 実験動物化 / 胚培養 / 新世界ウズラ / 旧世界ウズラ

Outline of Final Research Achievements

(1) Plumage color mutant in the large size Japanese quail was controlled by an autosomal dominant gene, fawn-2. The new strain homozygous for this gene, was developed and named JFN. (2) Embryonic genome activation was initiated around EG-K stage V in the Japanese quail. DAZL gene specifically expressed in the primordial germ cells showed about 100 bp difference in size between male and female in the Japanese quail. (3) Non-albino white plumage in the bobwhite quail was controlled by an autosomal recessive gene, rW (recessive white). The new strain homozygous for this gene was developed and named RW. (4) Distribution of glucagon-like peptide (GLP)-1-immunoreactive cells in the bobwhite quail was observed using immunohistochemical and morphometrical techniques. (5) Species specific PCR primers were designed to distinguish between Japanese quail and bobwhite quail.

Academic Significance and Societal Importance of the Research Achievements

経済協力開発機構（OECD)による鳥類を指標にした生態系への影響に関するテストガイドラインの試験法としてニホンウズラとともにコリンウズラの使用が推奨されている。肉用のニホンウズラから大型系統を造成した。コリンウズラを研究に特化した実験動物として遺伝的背景を制御するために野生種と区別できる（哺乳類の実験動物におけるラットと野生のドブネズミのように）非アルビノ系の白色羽装系統を造成し内外の研究者が実験動物として活用できるようにした。

Research Products

• [Int'l Joint Research] Suranaree University of Technology(タイ)
• [Int'l Joint Research] ソウル大学(韓国)
• [Int'l Joint Research] ソウル大学(韓国)
• [Int'l Joint Research] ソウル大学(韓国)
• [Journal Article] Distinct cell proliferation, myogenic differentiation, and gene expression in skeletal muscle myoblasts of layer and broiler chickens. (2019)
  • Author(s): Nihashi Y, Umezawa K, Shinji S, Hamaguchi Y, Kobayashi H, Kono T, Ono T, Kagami H, Takaya T.
  • Journal Title: Scientific Reports Volume: 9(1) Issue: 1 Pages: 16527-16527
  • DOI: 10.1038/s41598-019-52946-4
  • NAID: 120007099709
  • Peer Reviewed / Open Access
• [Journal Article] Glucagon-like peptide-1 receptor expression in pancreatic D cells of three avian species; white Leghorn chickens, bobwhite quails and common ostriches (2018)
  • Author(s): Watanabe, T., Hiramatsu K., Nishimura, K. and Ono, T.
  • Journal Title: Journal of Poultry Science Volume: 55 Pages: 199-203
  • Peer Reviewed / Open Access
• [Journal Article] Production of germline chimeric quails following spermatogonial cell transplantation in busulfan treated testis (2018)
  • Author(s): Kim,Y. M., Park, J. S., Yoon, J. W., Choi, H. J., Park, K. J., Ono, T. and Han, J. Y.
  • Journal Title: Asian Journal of Andrology Volume: 20 Issue: 4 Pages: 1-3
  • DOI: 10.4103/aja.aja_79_17
  • NAID: 120007183582
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The dynamic development of germ cells during chicken embryogenesis (2018)
  • Author(s): Yang, S. Y., Lee, H. J., Lee, H. C., Hwang, Y. S., Park, Y. H., Ono, T. and Han, J.Y.
  • Journal Title: Poultry Science Volume: 97 Issue: 2 Pages: 650-657
  • DOI: 10.3382/ps/pex316
  • NAID: 120007183584
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Size-dependent isolation of primordial germ cells from avian species (2017)
  • Author(s): Jung, K. M., Kim Y. M., Ono, T. and Han, J.Y.
  • Journal Title: Molecular Reproduction and Development Volume: 84 Issue: 6 Pages: 508-516
  • DOI: 10.1002/mrd.22802
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Autonomous xenogenic cell fusion of murine and chick skeletal muscles myoblasts (2017)
  • Author(s): Takaya, T., Nihashi, Y., Kojima, S., Ono, T. and Kagami, H.
  • Journal Title: Animal Science Journal Volume: 88 Issue: 11 Pages: 1880-1885
  • DOI: 10.1111/asj.12884
  • NAID: 120007099848
  • Peer Reviewed / Open Access
• [Journal Article] Genome modification technologies and their applications in avian species (2017)
  • Author(s): Lee, H. J., Kim, Y. M., Ono, T. and Han, J.Y.
  • Journal Title: International Journal of Molecular Sciences Volume: 18 Issue: 11 Pages: 1-13
  • DOI: 10.3390/ijms18112245
  • NAID: 120007183583
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Isolation, Characterization and In Vitro Culturing of Spermatogonial Stem Cells in Japanese Quail (Coturnix japonica) (2017)
  • Author(s): Pramod, R.K., Lee, B.R., Kim, Y.M., Lee, H.J., Park, Y.H., Ono, T., Lim, J.M., Han, J.Y.
  • Journal Title: Stem Cells and Development Volume: 1 Issue: 1 Pages: 60-70
  • DOI: 10.1089/scd.2016.0129
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Chicken stem cell factor enhances primordial germ cell proliferation cooperatively with fibroblast growth factor 2 (2016)
  • Author(s): Miyahara D, Oishi I, Makino R, Kurumisawa N, Nakaya R, Ono T, Kagami H, Tagami T
  • Journal Title: Journal of Reproduction and Development Volume: 62 Issue: 2 Pages: 143-149
  • DOI: 10.1262/jrd.2015-128
  • NAID: 130005146458
  • ISSN: 0916-8818, 1348-4400
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] The avian-specific small heat shock protein HSP25 is a constitutive protector against environmental stresses during blastoderm dormancy (2016)
  • Author(s): Hwang, Y.S., Ko, M.H., Kim, Y.M., Park, Y.H., Ono, T., Han, J.Y.
  • Journal Title: Scientific Reports Volume: 6 Issue: 1 Pages: 1-12
  • DOI: 10.1038/srep36704
  • NAID: 120007183585
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] 雄ウズラの生殖腺におけるDAZLの発現解析 (2020)
  • Author(s): 水島秀成・塚田光・笹浪知宏・小野珠乙・黒岩麻里
  • Organizer: 日本家禽学会春季大会
• [Presentation] ボブホワイト小腸の神経分布に関する免疫組織化学的研究 (2020)
  • Author(s): Md サラフディン・佐川昌宏・田村研人・平松浩二・小野珠乙
  • Organizer: 日本家禽学会春季大会
• [Presentation] RNA sequencing for global gene expression associated with proliferation and differentiation in chicken muscle myoblasts. Proceeding (2019)
  • Author(s): Nihashi Y, Umezawa K, Hamaguchi Y, Kobayashi H, Kono T, Ono T, Kagami H, Takaya T
  • Organizer: The 2nd International Conference on Tropical Animal Science and Technology
  • Int'l Joint Research
• [Presentation] Functional anatomy of the alimentary tract of northern bobwhites (2019)
  • Author(s): Tamura, K, Satoh M, Tanaka, H, Uchino, S, Salahuddin Md, Hitamatsu, K , Ono T
  • Organizer: The 2nd International Conference on Tropical Animal Science and Technology
  • Int'l Joint Research
• [Presentation] The role of inositol-trisphosphate receptors during egg activation in Japanese quail (2019)
  • Author(s): Mizushima S, Sasanami T, Ono T, Kuroiwa A
  • Organizer: The 2nd International Conference on Tropical Animal Science and Technology
  • Int'l Joint Research
• [Presentation] Novel technologies for chicken breeding by the stem cells (2019)
  • Author(s): Kusudo T, Molee, A, ,W.Molee, W, Kobayashi K, Asai A, Ban, Y, ,Ishige H, Kita R, Murakata N, Kawamura M, Takaya T, Ono T, Rikimaru K, Kagami H
  • Organizer: The 2nd International Conference on Tropical Animal Science and Technology
  • Int'l Joint Research
• [Presentation] ボブホワイト小腸の神経分布に関する免疫組織化学的研究 (2019)
  • Author(s): 田村研人・平松浩二・小野珠乙
  • Organizer: 日本家禽学会秋季大会
• [Presentation] ウズラの生殖腺における生殖細胞特異的遺伝子の発現解析 (2019)
  • Author(s): 水島秀成・佐藤望・塚田光・笹浪知宏・小野珠乙・黒岩麻里
  • Organizer: 第42回日本分子生物学会
• [Presentation] 肉用鶏と卵用鶏の骨格筋芽細胞における遺伝子発現の網羅的解析 (2019)
  • Author(s): 二橋佑磨・梅澤公二・浜口遥・小林久人・河野友宏・小野珠乙・鏡味裕・高谷智英
  • Organizer: 日本畜産学会第125回大会
• [Presentation] マウス-ニワトリキメラ胚作出の試み (2019)
  • Author(s): 浅井萌音・富岡郁夫・高谷智英・楠戸智也・伴佑里奈・小野珠乙・鏡味裕
  • Organizer: 日本畜産学会第125回大会
• [Presentation] ウズラの配偶子に発現するDNaseⅠの受精における機能 (2019)
  • Author(s): 水島秀成・笹浪知宏・小野珠乙・黒岩麻里
  • Organizer: 日本家禽学会春季大会
• [Presentation] ニワトリ骨格筋芽細胞におけるERVKの転写と近傍遺伝子群 (2019)
  • Author(s): 高谷智英・二橋佑磨・小野珠乙・鏡味裕
  • Organizer: 日本家禽学会春季大会
• [Presentation] ニワトリ骨格筋芽細胞の増殖・分化におけるエンケファリンの影響 (2019)
  • Author(s): 二橋佑磨・梅澤公二・小野珠乙・鏡味裕・高谷智英
  • Organizer: 日本家禽学会春季大会
• [Presentation] Primordial germ cell modification using Crispr/Cas9 for genome editing in aves (2018)
  • Author(s): J. Y., Lee, H. J., Park, K. J., Ono, T. and Kim, H.
  • Organizer: 11th Asian Pacific Poultry Conference
  • Int'l Joint Research
• [Presentation] Distinct expression of TLR genes between broiler and layer chick skeletal muscle myoblasts (2018)
  • Author(s): Nihashi, Y., Ono, T., Kagami, H. and Takaya, T.
  • Organizer: 11th Asian Pacific Poultry Conference
  • Int'l Joint Research
• [Presentation] ニワトリ骨格筋芽細胞におけるTLRリガンド依存的な炎症反応 (2018)
  • Author(s): 高谷智英・二橋佑磨・小野珠乙・鏡味裕
  • Organizer: 日本家禽学会春季大会
• [Presentation] 筋分化誘導型オリゴDNAとベルベリン複合体によるニワトリ筋芽細胞の分化誘導 (2018)
  • Author(s): 二橋佑磨・進士彩華・梅澤公二・下里剛士・小野珠乙・鏡味裕・高谷智英
  • Organizer: 日本家禽学会春季大会
• [Presentation] ニワトリ胚生殖細胞における後天的遺伝子発現制御機構の解析 (2017)
  • Author(s): 胡桃澤希未・宮原大地・牧野龍一・小野珠乙・鏡味裕
  • Organizer: 日本家禽学会
  • Place of Presentation: 神戸大学（兵庫県，神戸市）
  • Year and Date: 2017-03-30
• [Presentation] 人為的補助による体外培養胚からの雛の効率的産出 (2017)
  • Author(s): 佐藤彩水・清水信太・小野珠乙・鏡味裕
  • Organizer: 日本畜産学会
  • Place of Presentation: 神戸大学（兵庫県，神戸市）
  • Year and Date: 2017-03-29
• [Presentation] 哺乳類と鳥類の異種融合骨格筋の作出 (2017)
  • Author(s): 高谷智英・小島正太郎・小野珠乙・鏡味裕
  • Organizer: 日本畜産学会
  • Place of Presentation: 神戸大学（兵庫県，神戸市）
  • Year and Date: 2017-03-29
• [Presentation] 人為的補助による体外培養胚からの雛の効率的産出 (2017)
  • Author(s): 佐藤彩水・清水信太・小野珠乙・鏡味裕
  • Organizer: 日本畜産学会第122回大会
• [Presentation] 哺乳類と鳥類の異種融合骨格筋の作出 (2017)
  • Author(s): 高谷智英・小野珠乙・鏡味裕
  • Organizer: 日本畜産学会第122回大会
• [Presentation] 骨格筋芽細胞の増殖・分化能は肉用鶏と卵用鶏で異なる (2017)
  • Author(s): 二橋佑磨・小野珠乙・鏡味裕・高谷智英
  • Organizer: 日本家禽学会秋季大会
• [Presentation] ウズラ体細胞核移植胚の作成効率に及ぼす卵紫外線照射の影響 (2017)
  • Author(s): 水島秀成・小野珠乙・黒岩麻里・笹浪知宏
  • Organizer: 日本家禽学会秋季大会
• [Presentation] ニワトリキメラの作出と育種への応用の試み (2017)
  • Author(s): 鏡味裕・佐藤彩水・佐藤知弥・三木静華・小野珠乙・高谷智英・Amonrat Molee・Wittawat Molee・Supalak Prajan・Tran Hang・平松浩二
  • Organizer: 日本家禽学会秋季大会
• [Presentation] ニワトリとマウスの骨格筋芽細胞の自発的な異種細胞融合 (2017)
  • Author(s): 高谷智英・二橋佑磨・小島正太郎・小野珠乙・鏡味裕
  • Organizer: 日本家禽学会秋季大会
• [Presentation] ニワトリ受精卵・ニワトリ胚の新たな利用法の開拓 (2017)
  • Author(s): 鏡味裕・佐藤彩水・小野珠乙・兼子香代子・高谷智英
  • Organizer: 日本畜産学会第123回大会
• [Presentation] 乳酸菌由来オリゴＤＮＡによるニワトリ筋芽細胞の分化誘導 (2017)
  • Author(s): 二橋佑磨・進士彩華・小野珠乙・鏡味裕・梅澤公二・下里剛士・高谷智英
  • Organizer: 日本畜産学会第123回大会
• [Presentation] ニワトリ筋芽細胞におけるtoll-like receptor遺伝子群の発現 (2017)
  • Author(s): 二橋佑磨・小野珠乙・鏡味裕・高谷智英
  • Organizer: 北信越畜産学会第66回大会
• [Presentation] 乳酸菌オリゴＤＮＡによる筋分化誘導を活用した鶏肉増産法の開発 (2017)
  • Author(s): 二橋佑磨・進士彩華・小野珠乙・鏡味裕・梅澤公二・下里剛士・高谷智英
  • Organizer: 日本農芸化学会中部支部第180回支部例会
• [Presentation] ウズラ体細胞核移植胚の発生能 (2016)
  • Author(s): 水島秀成・小野珠乙・笹浪知宏
  • Organizer: 日本家禽学会
  • Place of Presentation: 静岡県もくせい会館（静岡県，静岡市）
  • Year and Date: 2016-09-15
• [Presentation] New and old world quail for germline transfer studies (2016)
  • Author(s): Ono, T., Hayashi, K., Mizushima, S., Han, J. Y. and Kagami, H.
  • Organizer: Tropical Animal Science and Reproduction
  • Place of Presentation: Bangkok, Thailand
  • Year and Date: 2016-07-26
  • Int'l Joint Research
• [Remarks] 動物発生遺伝学研究室ホームページ
  • URL: http://www.shinshu-u.ac.jp/faculty/agriculture/lab/ono_kagami/hasseiken.htm
• [Remarks] 信州大学農学部研究紹介
  • URL: https://www.shinshu-u.ac.jp/guidance/publication/summary/2019/agri-research/html5.html#page=15
• [Remarks] SOAR
  • URL: http://soar-rd.shinshu-u.ac.jp/profile/ja.WFfCPpkh.html
• [Remarks] SOAR
  • URL: http://soar-rd.shinshu-u.ac.jp/profile/en.WFfCPpkh.html
• [Remarks] 信州大学農学部 研究紹介デジタルパンフ
  • URL: http://www.shinshu-u.ac.jp/guidance/publication/summary/2016/agri-research/#page=17
• [Remarks] 動物発生遺伝学研究室ホームページ
  • URL: http://karamatsu.shinshu-u.ac.jp/lab/ono_kagami/hasseiken.htm