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Development of strain and utilization of new and old world quail species

Research Project

Project/Area Number 16K07986
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Animal production science
Research InstitutionShinshu University

Principal Investigator

Ono Tamao  信州大学, 学術研究院農学系, 教授 (10177264)

Project Period (FY) 2016-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywordsコリンウズラ / ニホンウズラ / 遺伝資源 / 実験動物化 / 胚培養 / 新世界ウズラ / 旧世界ウズラ
Outline of Final Research Achievements

(1) Plumage color mutant in the large size Japanese quail was controlled by an autosomal dominant gene, fawn-2. The new strain homozygous for this gene, was developed and named JFN. (2) Embryonic genome activation was initiated around EG-K stage V in the Japanese quail. DAZL gene specifically expressed in the primordial germ cells showed about 100 bp difference in size between male and female in the Japanese quail. (3) Non-albino white plumage in the bobwhite quail was controlled by an autosomal recessive gene, rW (recessive white). The new strain homozygous for this gene was developed and named RW. (4) Distribution of glucagon-like peptide (GLP)-1-immunoreactive cells in the bobwhite quail was observed using immunohistochemical and morphometrical techniques. (5) Species specific PCR primers were designed to distinguish between Japanese quail and bobwhite quail.

Academic Significance and Societal Importance of the Research Achievements

経済協力開発機構(OECD)による鳥類を指標にした生態系への影響に関するテストガイドラインの試験法としてニホンウズラとともにコリンウズラの使用が推奨されている。肉用のニホンウズラから大型系統を造成した。コリンウズラを研究に特化した実験動物として遺伝的背景を制御するために野生種と区別できる(哺乳類の実験動物におけるラットと野生のドブネズミのように)非アルビノ系の白色羽装系統を造成し内外の研究者が実験動物として活用できるようにした。

Report

(5 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (52 results)

All 2020 2019 2018 2017 2016 Other

All Int'l Joint Research (4 results) Journal Article (10 results) (of which Int'l Joint Research: 6 results,  Peer Reviewed: 10 results,  Open Access: 9 results,  Acknowledgement Compliant: 1 results) Presentation (32 results) (of which Int'l Joint Research: 7 results) Remarks (6 results)

  • [Int'l Joint Research] Suranaree University of Technology(タイ)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] ソウル大学(韓国)

    • Related Report
      2018 Research-status Report
  • [Int'l Joint Research] ソウル大学(韓国)

    • Related Report
      2017 Research-status Report
  • [Int'l Joint Research] ソウル大学(韓国)

    • Related Report
      2016 Research-status Report
  • [Journal Article] Distinct cell proliferation, myogenic differentiation, and gene expression in skeletal muscle myoblasts of layer and broiler chickens.2019

    • Author(s)
      Nihashi Y, Umezawa K, Shinji S, Hamaguchi Y, Kobayashi H, Kono T, Ono T, Kagami H, Takaya T.
    • Journal Title

      Scientific Reports

      Volume: 9(1) Issue: 1 Pages: 16527-16527

    • DOI

      10.1038/s41598-019-52946-4

    • NAID

      120007099709

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Glucagon-like peptide-1 receptor expression in pancreatic D cells of three avian species; white Leghorn chickens, bobwhite quails and common ostriches2018

    • Author(s)
      Watanabe, T., Hiramatsu K., Nishimura, K. and Ono, T.
    • Journal Title

      Journal of Poultry Science

      Volume: 55 Pages: 199-203

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Production of germline chimeric quails following spermatogonial cell transplantation in busulfan treated testis2018

    • Author(s)
      Kim,Y. M., Park, J. S., Yoon, J. W., Choi, H. J., Park, K. J., Ono, T. and Han, J. Y.
    • Journal Title

      Asian Journal of Andrology

      Volume: 20 Issue: 4 Pages: 1-3

    • DOI

      10.4103/aja.aja_79_17

    • NAID

      120007183582

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The dynamic development of germ cells during chicken embryogenesis2018

    • Author(s)
      Yang, S. Y., Lee, H. J., Lee, H. C., Hwang, Y. S., Park, Y. H., Ono, T. and Han, J.Y.
    • Journal Title

      Poultry Science

      Volume: 97 Issue: 2 Pages: 650-657

    • DOI

      10.3382/ps/pex316

    • NAID

      120007183584

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Size-dependent isolation of primordial germ cells from avian species2017

    • Author(s)
      Jung, K. M., Kim Y. M., Ono, T. and Han, J.Y.
    • Journal Title

      Molecular Reproduction and Development

      Volume: 84 Issue: 6 Pages: 508-516

    • DOI

      10.1002/mrd.22802

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Autonomous xenogenic cell fusion of murine and chick skeletal muscles myoblasts2017

    • Author(s)
      Takaya, T., Nihashi, Y., Kojima, S., Ono, T. and Kagami, H.
    • Journal Title

      Animal Science Journal

      Volume: 88 Issue: 11 Pages: 1880-1885

    • DOI

      10.1111/asj.12884

    • NAID

      120007099848

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Genome modification technologies and their applications in avian species2017

    • Author(s)
      Lee, H. J., Kim, Y. M., Ono, T. and Han, J.Y.
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 18 Issue: 11 Pages: 1-13

    • DOI

      10.3390/ijms18112245

    • NAID

      120007183583

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Isolation, Characterization and In Vitro Culturing of Spermatogonial Stem Cells in Japanese Quail (Coturnix japonica)2017

    • Author(s)
      Pramod, R.K., Lee, B.R., Kim, Y.M., Lee, H.J., Park, Y.H., Ono, T., Lim, J.M., Han, J.Y.
    • Journal Title

      Stem Cells and Development

      Volume: 1 Issue: 1 Pages: 60-70

    • DOI

      10.1089/scd.2016.0129

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Chicken stem cell factor enhances primordial germ cell proliferation cooperatively with fibroblast growth factor 22016

    • Author(s)
      Miyahara D, Oishi I, Makino R, Kurumisawa N, Nakaya R, Ono T, Kagami H, Tagami T
    • Journal Title

      Journal of Reproduction and Development

      Volume: 62 Issue: 2 Pages: 143-149

    • DOI

      10.1262/jrd.2015-128

    • NAID

      130005146458

    • ISSN
      0916-8818, 1348-4400
    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] The avian-specific small heat shock protein HSP25 is a constitutive protector against environmental stresses during blastoderm dormancy2016

    • Author(s)
      Hwang, Y.S., Ko, M.H., Kim, Y.M., Park, Y.H., Ono, T., Han, J.Y.
    • Journal Title

      Scientific Reports

      Volume: 6 Issue: 1 Pages: 1-12

    • DOI

      10.1038/srep36704

    • NAID

      120007183585

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 雄ウズラの生殖腺におけるDAZLの発現解析2020

    • Author(s)
      水島秀成・塚田光・笹浪知宏・小野珠乙・黒岩麻里
    • Organizer
      日本家禽学会春季大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ボブホワイト小腸の神経分布に関する免疫組織化学的研究2020

    • Author(s)
      Md サラフディン・佐川昌宏・田村研人・平松浩二・小野珠乙
    • Organizer
      日本家禽学会春季大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] RNA sequencing for global gene expression associated with proliferation and differentiation in chicken muscle myoblasts. Proceeding2019

    • Author(s)
      Nihashi Y, Umezawa K, Hamaguchi Y, Kobayashi H, Kono T, Ono T, Kagami H, Takaya T
    • Organizer
      The 2nd International Conference on Tropical Animal Science and Technology
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Functional anatomy of the alimentary tract of northern bobwhites2019

    • Author(s)
      Tamura, K, Satoh M, Tanaka, H, Uchino, S, Salahuddin Md, Hitamatsu, K , Ono T
    • Organizer
      The 2nd International Conference on Tropical Animal Science and Technology
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The role of inositol-trisphosphate receptors during egg activation in Japanese quail2019

    • Author(s)
      Mizushima S, Sasanami T, Ono T, Kuroiwa A
    • Organizer
      The 2nd International Conference on Tropical Animal Science and Technology
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Novel technologies for chicken breeding by the stem cells2019

    • Author(s)
      Kusudo T, Molee, A, ,W.Molee, W, Kobayashi K, Asai A, Ban, Y, ,Ishige H, Kita R, Murakata N, Kawamura M, Takaya T, Ono T, Rikimaru K, Kagami H
    • Organizer
      The 2nd International Conference on Tropical Animal Science and Technology
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ボブホワイト小腸の神経分布に関する免疫組織化学的研究2019

    • Author(s)
      田村研人・平松浩二・小野珠乙
    • Organizer
      日本家禽学会秋季大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ウズラの生殖腺における生殖細胞特異的遺伝子の発現解析2019

    • Author(s)
      水島秀成・佐藤望・塚田光・笹浪知宏・小野珠乙・黒岩麻里
    • Organizer
      第42回日本分子生物学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 肉用鶏と卵用鶏の骨格筋芽細胞における遺伝子発現の網羅的解析2019

    • Author(s)
      二橋佑磨・梅澤公二・浜口遥・小林久人・河野友宏・小野珠乙・鏡味裕・高谷智英
    • Organizer
      日本畜産学会第125回大会
    • Related Report
      2018 Research-status Report
  • [Presentation] マウス-ニワトリキメラ胚作出の試み2019

    • Author(s)
      浅井萌音・富岡郁夫・高谷智英・楠戸智也・伴佑里奈・小野珠乙・鏡味裕
    • Organizer
      日本畜産学会第125回大会
    • Related Report
      2018 Research-status Report
  • [Presentation] ウズラの配偶子に発現するDNaseⅠの受精における機能2019

    • Author(s)
      水島秀成・笹浪知宏・小野珠乙・黒岩麻里
    • Organizer
      日本家禽学会春季大会
    • Related Report
      2018 Research-status Report
  • [Presentation] ニワトリ骨格筋芽細胞におけるERVKの転写と近傍遺伝子群2019

    • Author(s)
      高谷智英・二橋佑磨・小野珠乙・鏡味裕
    • Organizer
      日本家禽学会春季大会
    • Related Report
      2018 Research-status Report
  • [Presentation] ニワトリ骨格筋芽細胞の増殖・分化におけるエンケファリンの影響2019

    • Author(s)
      二橋佑磨・梅澤公二・小野珠乙・鏡味裕・高谷智英
    • Organizer
      日本家禽学会春季大会
    • Related Report
      2018 Research-status Report
  • [Presentation] Primordial germ cell modification using Crispr/Cas9 for genome editing in aves2018

    • Author(s)
      J. Y., Lee, H. J., Park, K. J., Ono, T. and Kim, H.
    • Organizer
      11th Asian Pacific Poultry Conference
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Distinct expression of TLR genes between broiler and layer chick skeletal muscle myoblasts2018

    • Author(s)
      Nihashi, Y., Ono, T., Kagami, H. and Takaya, T.
    • Organizer
      11th Asian Pacific Poultry Conference
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] ニワトリ骨格筋芽細胞におけるTLRリガンド依存的な炎症反応2018

    • Author(s)
      高谷智英・二橋佑磨・小野珠乙・鏡味裕
    • Organizer
      日本家禽学会春季大会
    • Related Report
      2017 Research-status Report
  • [Presentation] 筋分化誘導型オリゴDNAとベルベリン複合体によるニワトリ筋芽細胞の分化誘導2018

    • Author(s)
      二橋佑磨・進士彩華・梅澤公二・下里剛士・小野珠乙・鏡味裕・高谷智英
    • Organizer
      日本家禽学会春季大会
    • Related Report
      2017 Research-status Report
  • [Presentation] ニワトリ胚生殖細胞における後天的遺伝子発現制御機構の解析2017

    • Author(s)
      胡桃澤希未・宮原大地・牧野龍一・小野珠乙・鏡味裕
    • Organizer
      日本家禽学会
    • Place of Presentation
      神戸大学(兵庫県,神戸市)
    • Year and Date
      2017-03-30
    • Related Report
      2016 Research-status Report
  • [Presentation] 人為的補助による体外培養胚からの雛の効率的産出2017

    • Author(s)
      佐藤彩水・清水信太・小野珠乙・鏡味裕
    • Organizer
      日本畜産学会
    • Place of Presentation
      神戸大学(兵庫県,神戸市)
    • Year and Date
      2017-03-29
    • Related Report
      2016 Research-status Report
  • [Presentation] 哺乳類と鳥類の異種融合骨格筋の作出2017

    • Author(s)
      高谷智英・小島正太郎・小野珠乙・鏡味裕
    • Organizer
      日本畜産学会
    • Place of Presentation
      神戸大学(兵庫県,神戸市)
    • Year and Date
      2017-03-29
    • Related Report
      2016 Research-status Report
  • [Presentation] 人為的補助による体外培養胚からの雛の効率的産出2017

    • Author(s)
      佐藤彩水・清水信太・小野珠乙・鏡味裕
    • Organizer
      日本畜産学会第122回大会
    • Related Report
      2017 Research-status Report
  • [Presentation] 哺乳類と鳥類の異種融合骨格筋の作出2017

    • Author(s)
      高谷智英・小野珠乙・鏡味裕
    • Organizer
      日本畜産学会第122回大会
    • Related Report
      2017 Research-status Report
  • [Presentation] 骨格筋芽細胞の増殖・分化能は肉用鶏と卵用鶏で異なる2017

    • Author(s)
      二橋佑磨・小野珠乙・鏡味裕・高谷智英
    • Organizer
      日本家禽学会秋季大会
    • Related Report
      2017 Research-status Report
  • [Presentation] ウズラ体細胞核移植胚の作成効率に及ぼす卵紫外線照射の影響2017

    • Author(s)
      水島秀成・小野珠乙・黒岩麻里・笹浪知宏
    • Organizer
      日本家禽学会秋季大会
    • Related Report
      2017 Research-status Report
  • [Presentation] ニワトリキメラの作出と育種への応用の試み2017

    • Author(s)
      鏡味裕・佐藤彩水・佐藤知弥・三木静華・小野珠乙・高谷智英・Amonrat Molee・Wittawat Molee・Supalak Prajan・Tran Hang・平松浩二
    • Organizer
      日本家禽学会秋季大会
    • Related Report
      2017 Research-status Report
  • [Presentation] ニワトリとマウスの骨格筋芽細胞の自発的な異種細胞融合2017

    • Author(s)
      高谷智英・二橋佑磨・小島正太郎・小野珠乙・鏡味裕
    • Organizer
      日本家禽学会秋季大会
    • Related Report
      2017 Research-status Report
  • [Presentation] ニワトリ受精卵・ニワトリ胚の新たな利用法の開拓2017

    • Author(s)
      鏡味裕・佐藤彩水・小野珠乙・兼子香代子・高谷智英
    • Organizer
      日本畜産学会第123回大会
    • Related Report
      2017 Research-status Report
  • [Presentation] 乳酸菌由来オリゴDNAによるニワトリ筋芽細胞の分化誘導2017

    • Author(s)
      二橋佑磨・進士彩華・小野珠乙・鏡味裕・梅澤公二・下里剛士・高谷智英
    • Organizer
      日本畜産学会第123回大会
    • Related Report
      2017 Research-status Report
  • [Presentation] ニワトリ筋芽細胞におけるtoll-like receptor遺伝子群の発現2017

    • Author(s)
      二橋佑磨・小野珠乙・鏡味裕・高谷智英
    • Organizer
      北信越畜産学会第66回大会
    • Related Report
      2017 Research-status Report
  • [Presentation] 乳酸菌オリゴDNAによる筋分化誘導を活用した鶏肉増産法の開発2017

    • Author(s)
      二橋佑磨・進士彩華・小野珠乙・鏡味裕・梅澤公二・下里剛士・高谷智英
    • Organizer
      日本農芸化学会中部支部第180回支部例会
    • Related Report
      2017 Research-status Report
  • [Presentation] ウズラ体細胞核移植胚の発生能2016

    • Author(s)
      水島秀成・小野珠乙・笹浪知宏
    • Organizer
      日本家禽学会
    • Place of Presentation
      静岡県もくせい会館(静岡県,静岡市)
    • Year and Date
      2016-09-15
    • Related Report
      2016 Research-status Report
  • [Presentation] New and old world quail for germline transfer studies2016

    • Author(s)
      Ono, T., Hayashi, K., Mizushima, S., Han, J. Y. and Kagami, H.
    • Organizer
      Tropical Animal Science and Reproduction
    • Place of Presentation
      Bangkok, Thailand
    • Year and Date
      2016-07-26
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Remarks] 動物発生遺伝学研究室ホームページ

    • URL

      http://www.shinshu-u.ac.jp/faculty/agriculture/lab/ono_kagami/hasseiken.htm

    • Related Report
      2019 Annual Research Report
  • [Remarks] 信州大学農学部研究紹介

    • URL

      https://www.shinshu-u.ac.jp/guidance/publication/summary/2019/agri-research/html5.html#page=15

    • Related Report
      2019 Annual Research Report
  • [Remarks] SOAR

    • URL

      http://soar-rd.shinshu-u.ac.jp/profile/ja.WFfCPpkh.html

    • Related Report
      2018 Research-status Report
  • [Remarks] SOAR

    • URL

      http://soar-rd.shinshu-u.ac.jp/profile/en.WFfCPpkh.html

    • Related Report
      2017 Research-status Report
  • [Remarks] 信州大学農学部 研究紹介デジタルパンフ

    • URL

      http://www.shinshu-u.ac.jp/guidance/publication/summary/2016/agri-research/#page=17

    • Related Report
      2016 Research-status Report
  • [Remarks] 動物発生遺伝学研究室ホームページ

    • URL

      http://karamatsu.shinshu-u.ac.jp/lab/ono_kagami/hasseiken.htm

    • Related Report
      2016 Research-status Report

URL: 

Published: 2016-04-21   Modified: 2021-02-19  

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