• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Characterization and utilization of SPACA1 proteins for the purpose of establishment of a new method to predict freezability of bull sperm before cryopreservation

Research Project

Project/Area Number 16K07990
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Animal production science
Research InstitutionKobe University

Principal Investigator

HARAYAMA HIROSHI  神戸大学, 農学研究科, 教授 (30281140)

Co-Investigator(Kenkyū-buntansha) 中嶋 昭雄  神戸大学, バイオシグナル総合研究センター, 准教授 (70397818)
坂瀬 充洋  兵庫県立農林水産技術総合センター, 北部農業技術センター, 課長 (70463396)
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords応用動物 / 畜産学 / 雄性繁殖学 / 精子 / 人工授精 / ウシ / 雄性繁殖能力 / 分子性状検査法
Outline of Final Research Achievements

The aims of the present study were to develop a new method to predict freezability of bull sperm before cryopreservation and to increase its reliability by disclosing molecular methodology. In this study, we succeeded in the development of the method to predict freezability of bull (Japanese Black bull) sperm before cryopreservation according to the criterion of the distribution of SPACA1 proteins. We also suggested that aberrant distribution of SPACA1 proteins is related to abnormal maturation of the sperm in the epididymis, and that low fertilizing ability of cryopreserved sperm with aberrant distribution of SPACA1 proteins is linked to poor results in the artificial insemination using them.

Academic Significance and Societal Importance of the Research Achievements

雌ウシを受胎させにくい精子を生産する種雄候補牛を,精子の分子マーカー(SPACA1タンパク質)を指標として利用することで客観的に特定できるようになった。またウシの雄性低繁殖症の原因分子のひとつを特定できた。これらの成果により,人工授精法によるウシの生産効率を向上でき,産肉能力または泌乳能力の高い優良個体をさらに増産できるようになる。本研究の成果は,食肉や牛乳などの畜産製品を社会に安定的に供給するための家畜の生産体制の改善に貢献すると期待される。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (18 results)

All 2019 2018 2017 2016

All Journal Article (7 results) (of which Peer Reviewed: 7 results,  Open Access: 2 results) Presentation (10 results) (of which Int'l Joint Research: 2 results,  Invited: 2 results) Book (1 results)

  • [Journal Article] Identification of isoforms of calyculin A-sensitive protein phosphatases which suppress full-type hyperactivation in bull ejaculated spermatozoa2019

    • Author(s)
      Arai Yuka, Sakase Mitsuhiro, Fukushima Moriyuki, Harayama Hiroshi
    • Journal Title

      Theriogenology

      Volume: 129 Pages: 46-53

    • DOI

      10.1016/j.theriogenology.2019.02.010

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Flagellar hyperactivation of bull and boar spermatozoa (Invited review)2018

    • Author(s)
      Harayama Hiroshi
    • Journal Title

      Reproductive Medicine and Biology

      Volume: 17 Issue: 4 Pages: 442-448

    • DOI

      10.1002/rmb2.12227

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Reconsideration of the evaluation criteria for bull ejaculated sperm motility in the context of rotation2018

    • Author(s)
      Yamada Ayano, Sakase Mitsuhiro, Fukushima Moriyuki, Harayama Hiroshi
    • Journal Title

      Journal of Reproduction and Development

      Volume: 64 Issue: 5 Pages: 377-384

    • DOI

      10.1262/jrd.2018-036

    • NAID

      130007497640

    • ISSN
      0916-8818, 1348-4400
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Variation among individual bulls in the distribution of acrosomal tyrosine-phosphorylated proteins in epididymal and ejaculated spermatozoa2017

    • Author(s)
      Miyuki M. Arai, Kenta Minami, Yukari Ogura, Nagisa Otsuka, Shohei Hama, Hiroshi Harayama, Mitsuhiro Sakase, Moriyuki Fukushima
    • Journal Title

      Reproduction, Fertility and Development

      Volume: 29 Issue: 7 Pages: 1297-1305

    • DOI

      10.1071/rd15483

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Characterization of extracellular Ca2+ -dependent full-type hyperactivation in ejaculated boar spermatozoa preincubated with a cAMP analog2017

    • Author(s)
      Nagisa Otsuka, Hiroshi Harayama
    • Journal Title

      Molecular Reproduction and Development

      Volume: 84 Issue: 11 Pages: 1203-1217

    • DOI

      10.1002/mrd.22921

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] 家畜精子鞭毛の超活性化運動に関する研究の現状(招待総説)2017

    • Author(s)
      原山 洋,水野洋平
    • Journal Title

      日本胚移植学雑誌

      Volume: 39 Pages: 159-167

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Protein biomarkers for male artificial insemination subfertility in bovine spermatozoa2017

    • Author(s)
      Hiroshi Harayama, Kenta Minami, Kazumi Kishida, Taichi Noda
    • Journal Title

      Reproductive Medicine and Development

      Volume: 16 Issue: 2 Pages: 89-98

    • DOI

      10.1002/rmb2.12021

    • NAID

      120006716622

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 活力検査法の改良を目的としたウシ射出精子の運動様式の観察2018

    • Author(s)
      山田綾乃,坂瀬充洋,福島護之,原山 洋
    • Organizer
      第111回日本繁殖生物学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] cAMP-PKAシグナル伝達活性の違いがウシ精子の運動様式に及ぼす影響 - 精子の活力検査における評価基準の見直し -2018

    • Author(s)
      山田綾乃,坂瀬充洋,福島護之,原山 洋
    • Organizer
      日本アンドロロジー学会第37回学術大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] ウシ精子での細胞外Ca2+依存的なFull-typeハイパーアクチベーションの抑制に機能するカリクリンA感受性プロテインホスファターゼアイソフォームの特定2018

    • Author(s)
      荒井佑香,原山 洋
    • Organizer
      第6回関西生殖医学集談会
    • Related Report
      2017 Research-status Report
  • [Presentation] ブタ精子での超活性化運動(Full-type HA)の開始におけるTRPC3チャネルの役割2017

    • Author(s)
      大塚 渚,原山 洋
    • Organizer
      第5回関西生殖医学集談会
    • Place of Presentation
      ハービスPLAZA(大阪府・大阪市)
    • Year and Date
      2017-03-04
    • Related Report
      2016 Research-status Report
  • [Presentation] Advanced techniques for the reproduction in the cattle2017

    • Author(s)
      Hiroshi Harayama
    • Organizer
      UGSVS lecture
    • Place of Presentation
      岐阜大学(岐阜県・岐阜市)
    • Year and Date
      2017-02-10
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] Calyculin A-sensitive protein phosphatases which are involved in the suppression for full-type hyperactivation of bovine sperm2017

    • Author(s)
      Yuka Arai, Mitsuhiro Sakase, Moriyuki Fukushima, Hiroshi Harayama
    • Organizer
      Fourth World Congress of Reproductive Biology
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Mechanism for the extracellular Ca2+-dependent occurrence of full-type hyperactivation in boar sperm treated with a cAMP analog2017

    • Author(s)
      Nagisa Otsuka, Hiroshi Harayama
    • Organizer
      Fourth World Congress of Reproductive Biology
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] ブタ精子におけるfull-type hyperactivationの開始制御へのTRPC3チャネルの関与2016

    • Author(s)
      大塚 渚,原山 洋
    • Organizer
      第109回日本繁殖生物学会大会
    • Place of Presentation
      麻布大学(神奈川県・相模原市)
    • Year and Date
      2016-09-12
    • Related Report
      2016 Research-status Report
  • [Presentation] ウシ精子のハイパーアクチベーションを抑制するカリクリンA感受性プロテインホスファターゼの特定2016

    • Author(s)
      荒井 佑香,坂瀬 充洋,福島 護之,原山 洋
    • Organizer
      第109回日本繁殖生物学会大会
    • Place of Presentation
      麻布大学(神奈川県・相模原市)
    • Year and Date
      2016-09-12
    • Related Report
      2016 Research-status Report
  • [Presentation] 先体タンパク質をマーカーとする哺乳類精子の分子性状検査法2016

    • Author(s)
      原山 洋
    • Organizer
      日本アンドロロジー学会35回学術大会 シンポジウム1:男性不妊症の分子メカニズム
    • Place of Presentation
      前橋テルサ(群馬県・前橋市)
    • Related Report
      2016 Research-status Report
    • Invited
  • [Book] 発生生物学 基礎から応用への展開 (第5章3節)2019

    • Author(s)
      原山 洋
    • Total Pages
      208
    • Publisher
      培風館
    • ISBN
      9784563078232
    • Related Report
      2018 Annual Research Report

URL: 

Published: 2016-04-21   Modified: 2020-03-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi