• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Molecular pathological study for mechanism for epilepsy in familial epileptic dog and epileptic focus in piliform lobe

Research Project

Project/Area Number 16K08020
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Veterinary medical science
Research InstitutionTottori University

Principal Investigator

MORITA Takehito  鳥取大学, 農学部, 教授 (70273901)

Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsepilepsy / てんかん / 神経新生 / イヌ / ラット / 梨状葉
Outline of Final Research Achievements

Epileptic seizure has been reported to enhance adult neurogenesis and induce aberrant synaptic reorganization in the human dentate gyrus in the hippocampal formation. However, adult neurogenesis in the extrahippocampal regions has not been well studied. To investigate seizure-enhanced neurogenesis in the extrahippocampal regions, we performed histological and immunohistochemical studies on the cerebrum of Sprague-Dawley ratstreated with intraperitoneal injection of pilocarpine (PL) to induce status epilepticus. Even though severe neuronal damage was found in the piriform cortex of rat having SE, immunohistochemistry for doublecortin (DCX) revealed an increase in number of immature neurons in the piriform cortex. Immunohistochemistry revealed increased expressions of synaptophysin and postsynaptic density protein 95 in the piriform cortex of rat having SE. These results suggested the enhanced neurogenesis and possible synaptic reorganization in the piriform cortex of the PL-treated rat.

Academic Significance and Societal Importance of the Research Achievements

梨状葉皮質はヒトのてんかん患者において新たな発作焦点として注目されている領域であり、その発作焦点の形成機序を解明することは、新たな発作焦点の形成抑制、さらに二次的なてんかん発作に対する治療に貢献できると考えられる。本研究ではピロカルピン投与によるてんかんモデルラットの発作後の梨状葉皮質に神経細胞壊死に引き続き、神経新生およびシナプスの再構築が生じる可能性を見出した。今後は、これらの組織改築にどのような分子が関わっているか明らかにすることが重要である。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (4 results)

All 2019 2018 2017

All Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (2 results)

  • [Journal Article] Autopsied case with MERRF/MELAS overlap syndrome accompanied by stroke‐like episodes localized to the precentral gyrus2019

    • Author(s)
      Miyahara Hiroaki、Matsumoto Shinjiro、Mokuno Kenji、Dei Rika、Akagi Akio、Mimuro Maya、Iwasaki Yasushi、Yoshida Mari
    • Journal Title

      Neuropathology

      Volume: 印刷中 Issue: 3 Pages: 135-143

    • DOI

      10.1111/neup.12551

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Enhanced neurogenesis and possible synaptic reorganization in the piriform cortex of adult rat following kainic acid-induced status epilepticus2018

    • Author(s)
      Sakurai M., Suzuki H., Tomita N., Sunden Y., Shimada A., Miyata H., Morita T.
    • Journal Title

      Neuropathology

      Volume: 38 Issue: 2 Pages: 135-143

    • DOI

      10.1111/neup.12445

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] リチウムピロカルピン投与てんかんモデルラットの 梨状葉皮質における神経新生に関する病理学的研究2017

    • Author(s)
      富田凪、岸大貴、寸田祐嗣、竹内 崇、森田剛仁
    • Organizer
      第4回日本獣医病理学専門家協会学術集会
    • Place of Presentation
      東京
    • Year and Date
      2017-03-30
    • Related Report
      2016 Research-status Report
  • [Presentation] リチウム・ピロカルピン投与てんかんモデルラットの梨状葉皮質における神経新生に関する病理学的研究2017

    • Author(s)
      富田凪 、岸大貴 、寸田祐嗣 、櫻井優 、竹内 崇 、森田剛仁
    • Organizer
      第4回日本獣医病理学専門家協会学術集会
    • Related Report
      2017 Research-status Report

URL: 

Published: 2016-04-21   Modified: 2020-03-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi