Development of infarction model induced by folic acid deficiency using a Microminipig atherosclerosis model
Project/Area Number |
16K08023
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Veterinary medical science
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Research Institution | Kagoshima University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
谷本 昭英 鹿児島大学, 医歯学域医学系, 教授 (10217151)
堀内 正久 鹿児島大学, 医歯学域医学系, 教授 (50264403)
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Research Collaborator |
Fukushima Syouji
Kanouchi Hiroaki
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Project Period (FY) |
2016-04-01 – 2019-03-31
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Project Status |
Completed (Fiscal Year 2018)
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Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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Keywords | 動脈硬化 / マイクロミニピッグ / ミニブタ / 心筋梗塞 / 脳梗塞 / 葉酸 / 高ホモシステイン血症 / メトトレキサート / ホモシステイン / 梗塞病変 / ミニブタモデル / 病理学 / 循環器・高血圧 / 栄養学 |
Outline of Final Research Achievements |
The aim of this study was development of myocardial and cerebral infarction models induced by folic acid deficiency using our atherosclerosis model induced by feeding a high fat and cholesterol diet (HFCD) for 8 weeks in Microminipigs. We investigated whether infarction was induced by the folic acid low-dosage high calorie diet feeding (FAL-HCD) and HFCD feeding with administration of methotrexate (MTX). HFCD+MTX induced hyperhomocysteinemia, hypofolate and acceleration of arteriosclerosis in aorta while FAL-HCD couldn’t induce hyperhomocysteinemia and hypofolate. Further studies are required to develop myocardial and cerebral infarction models of minipig, and to reveal the mechanism of arteriosclerosis acceleration induced by MTX.
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Academic Significance and Societal Importance of the Research Achievements |
日本の死因の上位を占める心筋梗塞および脳梗塞の主因は動脈硬化である。我々のヒトの生活習慣に準じた食餌制御性マイクロミニピッグ動脈硬化症モデルに、さらにヒトの生活習慣に起因する負荷を加え、梗塞を促進するモデルを開発することは、病態の機序解明や疾病の克服に貢献し、社会的意義は大きい。 本研究では心筋梗塞・脳梗塞モデルの開発には至らなかったが、開発のための改善点もみえてきた。また、メトトレキサート投与を負荷することにより、動脈硬化が増悪されたことが新たに発見され、今後この機序を解明することも、梗塞モデルへの発展にもつながり、学術的意義は大きい。
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Report
(4 results)
Research Products
(24 results)
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[Journal Article] Cholic Acid Enhances Visceral Adiposity, Atherosclerosis and Nonalcoholic Fatty Liver Disease in Microminipigs2017
Author(s)
Yamada S, Kawaguchi H, Yamada T, Guo X, Matsuo K, Hamada T, Miura N, Tasaki T, Tanimoto A.
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Journal Title
Journal of Atherosclerosis and Thrombosis
Volume: 24
Issue: 11
Pages: 1150-1166
DOI
NAID
ISSN
1340-3478, 1880-3873
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Microminipigs as a new experimental animal model for toxicological studies: comparative pharmacokinetics of perfluoroalkyl acids.2016
Author(s)
Guruge KS, Noguchi M, Yoshioka K, Yamazaki E, Taniyasu S, Yoshioka M, Yamanaka N, Ikezawa M, Tanimura N, Sato M, Yamashita N, Kawaguchi H.
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Journal Title
J Appl Toxicol
Volume: 36
Issue: 1
Pages: 68-75
DOI
Related Report
Peer Reviewed / Open Access
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