Pathological and genetical analyses of chicken Eimeria species during attenuations as a model of important protozoan parasites

• Project/Area Number: 16K08025
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Veterinary medical science
• Research Institution: Osaka Prefecture University
• Principal Investigator: Matsubayashi Makoto 大阪府立大学, 生命環境科学研究科, 准教授 (00321076)
• Project Period (FY): 2016-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: Eimeria / 原虫 / 弱毒化 / 比較ゲノム解析 / コクシジウム / シゾント / ゲノム解析 / 鶏 / Eimeria tenella / 無性生殖期 / 弱毒

Outline of Final Research Achievements

Chicken Eimeria spp. causing coccidiosis are attenuated by selective serial passages into chickens. However, the characteristics and molecular mechanisms of the attenuations remains unknown. The precocious strains showed rapid developments of the asexual stages and possessed smaller schizonts. By the comparative genomic analyses, some gene regions with nucleotide mutations were determined. These nucleotide mutations might be associated with attenuation of the eimerian parasites.

Academic Significance and Societal Importance of the Research Achievements

Eimeria 属原虫は、ヒトに対して重篤な症状を引き起こすマラリアやトキソプラズマ原虫と同じApicomplexa 門に属する。これまでに同門の原虫ワクチン開発において、弱毒株の原虫総体による免疫賦与を行うワクチン開発が次世代の抗原虫防除ツールとして模索されている。本成果により決定された遺伝子は、弱毒化重要遺伝子である可能性があり、他種原虫での応用解析が期待される。

Research Products

• [Journal Article] Transitions in morphological forms and rapid development of the asexual schizonts of Eimeria tenella through serial passaging in chicks (2019)
  • Author(s): Matsubayashi Makoto、Yamaguchi Hiroki、Hatta Takeshi、Kawahara Fumiya、Hatabu Toshimitsu、Iseki Hiroshi、Yamagishi Junya、Isobe Takashi、Teramoto Isao、Kaneko Akira、Kita Kiyoshi、Tsuji Naotoshi、Sasai Kazumi
  • Journal Title: Infection, Genetics and Evolution Volume: 75 Pages: 103993-103993
  • DOI: 10.1016/j.meegid.2019.103993
  • Peer Reviewed / Open Access
• [Journal Article] Susceptibility to Various Coccidiostats in the Murine Coccidian Parasite Eimeria krijgsmanni (2019)
  • Author(s): Inoue Kota、Tsujio Masashi、Matsubayashi Makoto、Inoue Ryota、Hatai Hitoshi、Andoh Masako、Abe Keisuke、Matsui Toshihiro、Matsuo Tomohide
  • Journal Title: Acta Parasitologica Volume: 64 Issue: 2 Pages: 418-422
  • DOI: 10.2478/s11686-019-00052-w
  • Peer Reviewed
• [Journal Article] NovelCharacteristics of Mitochondrial Electron Transport Chain from Eimeria tenella (2018)
  • Author(s): Matsubayashi M, Inaoka DK, Komatsuya K, Hatta T, Kawahara F, Sakamoto K,Hikosaka K, Yamagishi J, Sasai K, Shiba T, Harada S, Tsuji N, Kita K.
  • Journal Title: Genes (Basel) Volume: 10 Issue: 1 Pages: 1-1
  • DOI: 10.3390/genes10010029
  • Peer Reviewed / Open Access
• [Journal Article] Surveillance of Eimeria species in wild Japanese rock ptarmigans, Lagopus muta japonica, and insight into parasitic seasonal life cycle at timberline regions of the Japanese Alps. (2018)
  • Author(s): Makoto Matsubayashi, Sayaka Tsuchida, Kazunari Ushida, Koichi Murata.
  • Journal Title: International Journal for Parasitology: Parasites and Wildlife Volume: 7(2) Issue: 2 Pages: 134-140
  • DOI: 10.1016/j.ijppaw.2018.03.004
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] First surveillance and molecular identification of the Cryptosporidium skunk genotype and Cryptosporidium parvum in wild raccoons (Procyon lotor) in Osaka, Japan. (2018)
  • Author(s): Koji Hattori, Takuto Donomoto, Tilusha Manchanayake, Tomoyuki Shibahara, Kazumi Sasai, Makoto Matsubayashi.
  • Journal Title: Parasitology Research Volume: 117(11) Issue: 11 Pages: 3669-3674
  • DOI: 10.1007/s00436-018-6089-y
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Molecular identification of two Eimeria species, E. uekii and E. raichoi as type B, in wild Japanese rock ptarmigans, Lagopus muta japonica. (2018)
  • Author(s): Makoto Matsubayashi, Sayaka Tsuchida, Atsushi Kobayashi, Tomoyuki Shibahara, Hiroshi Nakamura, Koichi Murata, Kazunari Ushida.
  • Journal Title: International Journal for Parasitology: Parasites and Wildlife. Volume: 7(3) Issue: 3 Pages: 243-250
  • DOI: 10.1016/j.ijppaw.2018.06.005
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] First detection and molecular identification of Entamoeba bovis from Japanese cattle (2018)
  • Author(s): Makoto Matsubayashi, Yuu Matsuura, Satoko Nukata, Yuusuke Daizi, Tomoyuki Shibahara, Isao Teramoto, Tomohide Matsuo, Shigehiko Uni, Takeshi Hatta, Akira Kaneko, Naotoshi Tsuji, Kazumi Sasai.
  • Journal Title: Parasitology Research Volume: 117(1) Issue: 1 Pages: 339-342
  • DOI: 10.1007/s00436-017-5689-2
  • Peer Reviewed / Open Access
• [Journal Article] Development of molecular diagnostic protocols for detecting three types of Entamoeba from diarrheal and asymptomatic pigs and environmental moist soils (2017)
  • Author(s): Yoshimasa Hirashima, Tilusha Manchanayake, Takahisa Yano, Syoei Kitahara, Terunori Koreeda, Syunsuke Kamimura, Kazumi Sasai, Makoto Matsubayashi, Tomoyuki Shibahara.
  • Journal Title: Parasitology Research Volume: 116(7) Issue: 7 Pages: 2001-2007
  • DOI: 10.1007/s00436-017-5483-1
  • Peer Reviewed / Open Access
• [Journal Article] Pathogenic characteristics of a novel intranuclear coccidia in Japanese black calves and its genetic identification as Eimeria subspherica (2017)
  • Author(s): Terunori Koreeda, Tomo Kawakami, Ayako Okada, Yoshimasa Hirashima, Naoto Imai, Kazumi Sasai, Shogo Tanaka, Makoto Matsubayashi, Tomoyuki Shibahara.
  • Journal Title: Parasitology Research Volume: 116(11) Issue: 11 Pages: 3243-3247
  • DOI: 10.1007/s00436-017-5629-1
  • Peer Reviewed / Open Access
• [Journal Article] A Large-Scale Survey of <i>Trichinella</i> spp. Infection in Japanese Wild Boars (2017)
  • Author(s): Hatta T, Imamura K, Yamamoto T, Matsubayashi M, Tsuji N, Tsutsui T.
  • Journal Title: Japanese Journal of Infectious Diseases Volume: 70 Issue: 2 Pages: 219-220
  • DOI: 10.7883/yoken.JJID.2016.258
  • NAID: 130005475421
  • ISSN: 1344-6304, 1884-2836
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Report of fatal mixed infection with Cryptosporidium parvum and Giardia intestinalis in neonatal calves (2017)
  • Author(s): Yuu Matsuura, Makoto Matsubayashi, Satoko Nukata, Tomoyuki Shibahara, Osamu Ayukawa, Yasuko Kondo, Tomohide Matsuo, Shigehiko Uni, Masaru Furuya, Hiroyuki Tani, Naotoshi Tsuji, Kazumi Sasai.
  • Journal Title: Acta Parasitologica Volume: 62(1) Issue: 1 Pages: 214-220
  • DOI: 10.1515/ap-2017-0026
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Initial development of Babesia ovata in the tick midgut (2017)
  • Author(s): Maeda, H., Hatta, T., Alim, M. A., Tsubokawa, D., Mikami, F., Kusakisako, K., Matsubayashi, M., Umemiya-Shirafuji, R., Tsuji, N. and Tanaka, T.
  • Journal Title: Vet. Prasitol. Volume: 233 Pages: 39-42
  • DOI: 10.1016/j.vetpar.2016.11.020
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Identification of Eimeria acervulina conoid antigen using chicken monoclonal antibody. (2016)
  • Author(s): Makoto Matsubayashi, Chisa Minoura, Shintaro Kimura, Hiroyuki Tani, Masaru Furuya, Hyun S. Lillehoj, Haruo Matsuda, Shigeo Takenaka, Takeshi Hatta, Naotoshi Tsuji, Kazumi Sasai.
  • Journal Title: Parasitology Research Volume: 115(11) Pages: 4123-4128
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] First report of mixed Entamoeba polecki (ST 1) and E. suis infection in piglets shedding abnormalfeces by histopathological and molecular surveys. (2016)
  • Author(s): Matsubayashi M, Sasagawa Y, Aita T, Tokoro M, Haritani M, Shibahara T.
  • Journal Title: Acta Parasitol. Volume: 61 Issue: 4 Pages: 665-670
  • DOI: 10.1515/ap-2016-0093
  • Peer Reviewed / Open Access
• [Journal Article] Detection of kobuvirus RNA in Japanese domestic dogs (2016)
  • Author(s): Takehisa Soma, Makoto Matsubayashi, Kazumi Sasai.
  • Journal Title: Journal of Veterinary Medical Science Volume: 78(11) Pages: 1731-1735
  • NAID: 130005170610
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Establishment of a novel tick-Babesia experimental infection mode (2016)
  • Author(s): Maeda, H., Hatta, T., Alim, M. A., Tsubokawa, D., Mikami, F., Matsubayashi, M., Miyoshi, T., Umemiya-Shirafuji, R., Kawazu, S., Igarashi, I., Mochizuki, M., Tsuji, N. and Tanaka, T.
  • Journal Title: Sci. Rep. Volume: 6 Issue: 1 Pages: 37039-37039
  • DOI: 10.1038/srep37039
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] 豚のEntamoeba属原虫感染による潰瘍性大腸炎. (2016)
  • Author(s): 矢口裕司，松林 誠，板橋知子，藤井勇紀，小貫登輝夫，笹井和美，小林 勝，芝原友幸.
  • Journal Title: 日本獣医師会雑誌 Volume: 7(69) Pages: 389-393
  • NAID: 130005405150
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Eimeria tenella 弱毒株の作出と比較ゲノム解析による弱毒化分子機構の解明 (2018)
  • Author(s): 松林誠、川原史也、山岸潤也、八田岳士、畑生俊光、山口浩貴、寺本勲、金子明、磯部尚、北潔、辻尚利、笹井和美.
  • Organizer: 第161回日本獣医学会
• [Presentation] Eimeria tenella 弱毒株の作出と比較ゲノム解析による弱毒化分子機構解明の試み (2018)
  • Author(s): 松林 誠、川原史也、山岸潤也、八田岳士、畑生俊光、寺本 勲、金子 明、磯部 尚、北 潔、辻 尚利、笹井和美.
  • Organizer: 第87回日本寄生虫学会大会
• [Presentation] 日本産業動物獣医学会 シンポジウム 「牛の下痢症に関する最近の知見」 産シンポⅦ-2. 消化管寄生原虫の診断と対策および今後の課題～特にクリプトスポリジウムについて～. (2017)
  • Author(s): 松林 誠.
  • Organizer: 平成28年度日本獣医師会 獣医学術学会 年次大会
  • Place of Presentation: 石川県立音楽堂等(石川)
  • Year and Date: 2017-02-24
  • Invited
• [Presentation] 抗鶏コクシジウム症薬の開発を目指したEimeria tenella Dihydroorotate dehydrogenaseの発現・精製と結晶化. (2016)
  • Author(s): 長濱 まどか, 佐藤 暖, 志波 智生, 松林 誠, 稲岡 ダニエル健, 辻 尚利, 北 潔, 原田 繁春.
  • Organizer: 第88回日本生化学会
  • Place of Presentation: 京都産業大学(京都)
  • Year and Date: 2016-12-01
• [Presentation] Cryptosporidium parvum における呼吸鎖関連酵素の特性解析. (2016)
  • Author(s): 高橋 和瑛、松林 誠、稲岡 ダニエル 健、八田 岳士、寺本 勲、金子 明、所 正治、古家 優、谷 浩行、辻 尚利、笹井 和美、北 潔.
  • Organizer: 第157回日本獣医学会
  • Place of Presentation: 日本大学(神奈川)
  • Year and Date: 2016-09-06
• [Presentation] 豚に寄生するEntamoeba 属原虫の遺伝子型の同定と病原性. (2016)
  • Author(s): 松林 誠、村越 奈穂子、小松 徹也、所 正治、播谷 亮、芝原 友幸.
  • Organizer: 第157回日本獣医学会
  • Place of Presentation: 日本大学(神奈川)
  • Year and Date: 2016-09-06
• [Presentation] マウス寄生性コクシジウムEimeria krijgsmanni感染の再活性化. (2016)
  • Author(s): 水野 真伸、松林 誠、辻尾 祐志、正谷 達謄、田仲 哲也、松井 利博、松尾 智英.
  • Organizer: 第157回日本獣医学会
  • Place of Presentation: 日本大学(神奈川)
  • Year and Date: 2016-09-06
• [Presentation] Babesia ovata原虫におけるマダニ中腸形態へのステージシフト誘導実験. (2016)
  • Author(s): 前田 大輝、八田 岳士、坪川 大悟、三上 房子、松林 誠、白藤（梅宮）梨可、辻 尚利、田仲 哲也.
  • Organizer: 第157回日本獣医学会
  • Place of Presentation: 日本大学(神奈川)
  • Year and Date: 2016-09-06
• [Presentation] 国内30府県で捕獲されたイノシシにおける旋毛虫（トリヒナ）保有調査の結果について. (2016)
  • Author(s): 八田 岳士、今村 憲吉、山本 健久、松林 誠、辻 尚利、筒井 俊之.
  • Organizer: 第157回日本獣医学会
  • Place of Presentation: 日本大日本大学(神奈川)
  • Year and Date: 2016-09-06
• [Book] 動物寄生虫病学（四訂版） (2019)
  • Author(s): 板垣 匡、藤﨑 幸藏 (監修)、松林 誠 (分担)
  • Total Pages: 368
  • Publisher: 朝倉書店
  • ISBN: 4254460376
• [Book] 動物の感染症 ＜第四版＞ (2019)
  • Author(s): 明石博臣 (監修)、松林 誠 (分担)
  • Total Pages: 352
  • Publisher: 近代出版
  • ISBN: 4874022502
• [Book] 動物病院スタッフのための犬と猫の感染症ガイド 適切でわかりやすいインフォームのために (2019)
  • Author(s): 小沼 守, 前田 健, 佐藤 宏 (監修)、松林 誠 (分担)
  • Total Pages: 166
  • Publisher: 緑書房
  • ISBN: 4895313689
• [Book] 臨床獣医師のための犬と猫の感染症診療 (2019)
  • Author(s): 前田 健, 佐藤 宏 (監修)、松林 誠 (分担)
  • Total Pages: 448
  • Publisher: 緑書房
  • ISBN: 4895313514
• [Book] 鶏病検査マニュアル (2016)
  • Author(s): 鶏病研究会 (分担 松林 誠)
  • Total Pages: 73
  • Publisher: 創文印刷工業株式会社