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Development of novel alpha-glucosidase inhibitors suppressing coronavirus replication.

Research Project

Project/Area Number 16K08032
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Veterinary medical science
Research InstitutionNippon Veterinary and Life Science University

Principal Investigator

Ujike Makoto  日本獣医生命科学大学, 獣医学部, 准教授 (50415478)

Co-Investigator(Kenkyū-buntansha) 袴田 航  日本大学, 生物資源科学部, 准教授 (10333337)
Research Collaborator Matsuyama Shutoku  
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsコロナウイルス / 抗ウイルス薬 / 小胞体αグルコシダーゼ阻害薬 / ウイルス学 / 新規糖鎖付加阻害薬
Outline of Final Research Achievements

Endoplasmic reticulum (ER) alpha-glucosidase inhibitors (AGI) inhibit host ER alpha-glucosidases, resulting in the suppression of host glycoprotein synthesis. Because most enveloped viruses including coronavirus (CoV) contain glycoproteins, AGI would efficiently disrupt the morphogenesis of a broad spectrum of enveloped viruses in infected cells. In this study, we identified six novel AGIs which have a novel framework by screening from the compound library and found that these AGIs showed strong antiviral activity against animal or human CoVs. It was first thought that the anti-CoV activity by novel AGIs was due to the inhibition of the structural protein, viral spike glycoproteins. However, we found that novel AGIs specifically inhibited not only spike glycoprotein but viral non-glycoproteins and even viral transcription activity. These data suggested that novel AGIs showed anti-CoV activity by a distinct mechanism from conventional one.

Academic Significance and Societal Importance of the Research Achievements

CoV科は、監視伝染病である豚伝染性胃腸炎及び伝染性気管支炎ウイルスや、近年国内で大流行した豚流行性下痢ウイルス、実験動物取扱時に問題となるマウス肝炎ウイルス、ペットの猫や野生ネコ属に致死的な病態を引き起こす猫伝染性腹膜炎ウイルスを含む。また、ヒトにおいては高い致死率をもつ重症呼吸器症候群や中東呼吸器症候群CoVを含み医学・獣医学領域で重要なウイルスである。しかしながら、現在でも効果的な抗CoV薬は存在しない。本研究により見出された、幅広いCoVに効果をもつAGIsが臨床応用されれば動物の衛生及びヒトの公衆衛生に大きな影響を与えると考えられる。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (14 results)

All 2018 2017 2016

All Journal Article (5 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 5 results,  Open Access: 1 results,  Acknowledgement Compliant: 2 results) Presentation (9 results)

  • [Journal Article] Identification of Small-Molecule Inhibitors of Human Golgi Mannosidase <i>via</i> a Drug Repositioning Screen2018

    • Author(s)
      Koyama R, Hakamata W, Hirano T, Nishio T.
    • Journal Title

      Chemical and Pharmaceutical Bulletin

      Volume: 66 Issue: 6 Pages: 678-681

    • DOI

      10.1248/cpb.c17-01009

    • NAID

      130007382341

    • ISSN
      0009-2363, 1347-5223
    • Year and Date
      2018-06-01
    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Chemoenzymatic synthesis of sucuronic acid using d-glucurono-6,3-lactone and sucrose as raw materials, and properties of the product.2018

    • Author(s)
      Hosaka H, Mizoguchi S, Tashiro M, Fujimoto T, Hirano T, Hakamata W, Nishio T.
    • Journal Title

      Enzyme Microb Technol.

      Volume: 110 Pages: 53-60

    • DOI

      10.1016/j.enzmictec.2017.12.006

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Synthesis of Chitin Oligosaccharides Using Dried Stenotrophomonas maltophilia Cells Containing a Transglycosylation Reaction-Catalyzing β-N-Acetylhexosaminidase as a Whole-Cell Catalyst.2018

    • Author(s)
      Uehara A, Takahashi N, Moriyama M, Hirano T, Hakamata W, Nishio T.
    • Journal Title

      Appl Biochem Biotechnol.

      Volume: 184(2) Issue: 2 Pages: 673-684

    • DOI

      10.1007/s12010-017-2585-2

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] The contribution of the cytoplasmic retrieval signal of severe acute respiratory syndrome coronavirus to intracellular accumulation of S proteins and incorporation of S protein into virus-like particles.2016

    • Author(s)
      Ujike M, Huang C, Shirato K, Makino S, Taguchi F.
    • Journal Title

      J Gen Virol.

      Volume: 印刷中 Issue: 8 Pages: 1853-64

    • DOI

      10.1099/jgv.0.000494

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Phylogenetic and antigenic characterization of newly isolated porcine epidemic diarrhea viruses in Japan.2016

    • Author(s)
      Islam MT, Kubota T, Ujike M, Yahara Y, Taguchi F.
    • Journal Title

      Virus Res

      Volume: 222 Pages: 113-9

    • DOI

      10.1016/j.virusres.2016.06.006

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] 牛トロウイルスの血球凝集素‐エステラーゼ(HE)遺伝子の安定性に関する研究2018

    • Author(s)
      氏家誠、江藤由佳、木暮茉希、イスラム・タイムール、田口文広
    • Organizer
      第66回日本ウイルス学会学術集会(京都)
    • Related Report
      2018 Annual Research Report
  • [Presentation] 牛トロウイルスヌクレオカプシド蛋白質のCRM1非依存的核外移行シグナルに関する研究2018

    • Author(s)
      木田萌子、木村留華、岩田修二、松永惟、田口文広、氏家誠
    • Organizer
      第66回日本ウイルス学会学術集会(京都)
    • Related Report
      2018 Annual Research Report
  • [Presentation] 牛トロウイルスのMembrane(M)蛋白質の細胞内局在に関する研究2018

    • Author(s)
      鈴木友美、田口文広、氏家誠
    • Organizer
      第66回日本ウイルス学会学術集会(京都)
    • Related Report
      2018 Annual Research Report
  • [Presentation] 牛トロウイルスヌクレオカプシド蛋白質の核輸送機構に関する研究2017

    • Author(s)
      岩田修二、松永惟、河内悠華子、田口文広、氏家誠
    • Organizer
      第65回日本ウイルス学会学術集会
    • Related Report
      2017 Research-status Report
  • [Presentation] 新規小胞体α-グルコシダーゼ阻害薬による抗コロナウイルス機構に関する研究2017

    • Author(s)
      小酒さおり、坪内千春、上間駿、恩田桃子、松山州徳、袴田航、氏家誠
    • Organizer
      第65回日本ウイルス学会学術集会
    • Related Report
      2017 Research-status Report
  • [Presentation] Inverse correlation between innate immune responses and pathogenicity of influenza H7N9 virus that is adapted to mice2017

    • Author(s)
      Teruki Miyazaki, Kimimasa Takahashi, Makoto Ujike, Akira Ainai, Hideki Hasegawa, Noriyo Nagata, Takato Odagiri, Masato Tashiro, Hideki Asanuma
    • Organizer
      第65回日本ウイルス学会学術集会
    • Related Report
      2017 Research-status Report
  • [Presentation] Phylogenetic and antigenic characterization of newly isolated porcine epidemic diarrhea virus (PEDV) in Japan2016

    • Author(s)
      Islam MT, Kubota T, Ujike M, Yahara Y, Taguchi F.
    • Organizer
      The 64th Annual Meeting of the Japanese Society for Virology
    • Place of Presentation
      Sapporo convention center (Hokkaido, Sapporo )
    • Year and Date
      2016-10-23
    • Related Report
      2016 Research-status Report
  • [Presentation] 小胞体α-グルコシダーゼ阻害薬による抗コロナウイルス機構に関する研究2016

    • Author(s)
      氏家誠、松永唯、荒井 詩織、松山州徳、袴田 航
    • Organizer
      第159回日本獣医学会学術集会
    • Place of Presentation
      日本大学生物資源学部(神奈川県藤沢市)
    • Year and Date
      2016-09-06
    • Related Report
      2016 Research-status Report
  • [Presentation] 牛トロウイルスN蛋白質の核輸送調節に関する研究2016

    • Author(s)
      伊藤咲、松永惟、河内悠華子、田口文広、氏家誠
    • Organizer
      第159回日本獣医学会学術集会
    • Place of Presentation
      日本大学生物資源学部(神奈川県藤沢市)
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2020-03-30  

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