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Analysis of polymorphism of bovine major histocompatibility complex (BoLA) haplotypes associated with enzootic bovine leukemia

Research Project

Project/Area Number 16K08039
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Veterinary medical science
Research InstitutionJumonji University (2018)
Institute of Physical and Chemical Research (2016-2017)

Principal Investigator

Takeshima Shinnosuke  十文字学園女子大学, 人間生活学部, 教授 (60342812)

Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords網羅的多型解析 / ウシゲノム解析 / 抗病性動物 / 牛白血病 / ウシ主要組織適合遺伝子複合体 / BoLA / DRB3 / 次世代シーケンサー / 次世代シークエンサー / ARS-UCD1.2 / UMD3.1 / BoLA-DRB3タイピング / ウシ主要組織適合遺伝子複合体(BoLA) / 牛白血病ウイルス / 疾患感受性 / ゲノムリシークエンス / 畜産学 / 感染症 / ウィルス / 遺伝子 / 獣医学
Outline of Final Research Achievements

The aim of this study was developing a method to analyze the bovine major histocompatibility complex (BoLA) region, which is believed to be correlated with various bovine diseases such as bovine leukemia, mastitis and parasitemia. Because the BoLA region is extremely diverse, in this study, we collected haplotypes related to bovine leukemia in particular, and worked on the analysis of target resequencing using those individuals. In the final year of this study, we also examine the bovine genome reference sequence that has been revised for the first time in 15 years. We succeeded in developing a typing method that was suitable for searching for related SNPs.

Academic Significance and Societal Importance of the Research Achievements

主要組織適合遺伝子(MHC)はヒトにおいても100にも及ぶ疾患との相関性が示されており、極めて重要な遺伝子領域である。ウシにおいても、数多くの疾病との相関性が示されており、遺伝子領域の多様性の全容をタイピングする事は重要である。本研究により示された新たなタイピング法により、これまでウシMHCのうち、一部しか明らかにできなかった多様性の全容が解析可能となり、既に多く報告されている牛白血病や、乳房炎、寄生虫血症など、真に相関する遺伝子の特定が可能となる。ワクチン戦略や抵抗性遺伝子の情報を使用したウシのブランド化、感染症対策や、これまで飼養困難だった地域でのウシの飼育などその応用範囲はとても広い。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (32 results)

All 2019 2018 2017 2016 Other

All Int'l Joint Research (2 results) Journal Article (14 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 14 results,  Open Access: 5 results,  Acknowledgement Compliant: 5 results) Presentation (15 results) (of which Int'l Joint Research: 9 results,  Invited: 1 results) Patent(Industrial Property Rights) (1 results)

  • [Int'l Joint Research] 国立ラプラタ大学(アルゼンチン)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] 国立ラプラタ大学(アルゼンチン)

    • Related Report
      2017 Research-status Report
  • [Journal Article] Bovine leukemia virus proviral load is more strongly associated with bovine major histocompatibility complex class II DRB3 polymorphism than with DQA1 polymorphism in Holstein cow in Japan2019

    • Author(s)
      Takeshima Shin-nosuke、Ohno Ayumu、Aida Yoko
    • Journal Title

      Retrovirology

      Volume: 16 Issue: 1 Pages: 14-14

    • DOI

      10.1186/s12977-019-0476-z

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Breeding Bulls as a Source of Bovine Leukemia Virus for Beef Herds2019

    • Author(s)
      Oscar J Benitez-Rojas, Jennifer Roberts, Bo Norby, Paul Bartlett, Shin-Nosuke Takeshima, Sonoko Watanuki, Yoko Aida, Daniel Grooms
    • Journal Title

      Journal of the American Veterinary Medical Association

      Volume: 254 Issue: 11 Pages: 1335-1340

    • DOI

      10.2460/javma.254.11.1335

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Development of a new recombinant p24 ELISA system for diagnosis of bovine leukemia virus in serum and milk2018

    • Author(s)
      Lanlan Bai, Kana Yokoyama, Sonoko Watanuki, Hiroshi Ishizaki, Shin-nosuke Takeshima, Yoko Aida
    • Journal Title

      Archives of Virology

      Volume: 164 Issue: 1 Pages: 201-211

    • DOI

      10.1007/s00705-018-4058-5

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Variations in the viral genome and biological properties of bovine leukemia virus wild-type strains2018

    • Author(s)
      Murakami Hironobu、Uchiyama Jumpei、Suzuki Chihiro、Nikaido Sae、Shibuya Kaho、Sato Reiichiro、Maeda Yosuke、Tomioka Michiko、Takeshima Shin-nosuke、Kato Hajime、Sakaguchi Masahiro、Sentsui Hiroshi、Aida Yoko、Tsukamoto Kenji
    • Journal Title

      Virus Research

      Volume: 253 Pages: 103-111

    • DOI

      10.1016/j.virusres.2018.06.005

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genetic diversity of BoLA-DRB3 in South American Zebu cattle populations.2018

    • Author(s)
      Shin-nosuke Takeshima, Claudia Corbi-Botto, Guillermo Giovambattista, Yoko Aida
    • Journal Title

      BMC Genetics

      Volume: 19 Issue: 1 Pages: 33-33

    • DOI

      10.1186/s12863-018-0618-7

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Genetic Diversity of BoLA-DRB3 in South American Zebu Cattle Populations2018

    • Author(s)
      Takeshima SN, Corbi-Botto C, Giovambattista G, Aida Y
    • Journal Title

      BMC Genetics

      Volume: 印刷中

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Single nucleotide polymorphisms in the bovine MHC region of Japanese Black cattle are associated with bovine leukemia virus proviral load2017

    • Author(s)
      Shin-nosuke Takeshima, Shinji Sasaki, Polat Meripet, Yoshikazu Sugimoto, Yoko Aida
    • Journal Title

      Retrovirology

      Volume: 14(1):24 Issue: 1

    • DOI

      10.1186/s12977-017-0348-3

    • Related Report
      2017 Research-status Report 2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] BLVのワクチン開発 (特集 牛白血病ウイルス(BLV)に対する理研の挑戦)2017

    • Author(s)
      竹嶋 伸之輔, 大附 寛幸, 白 らんらん, 間 陽子
    • Journal Title

      獣医畜産新報

      Volume: 70(12) Pages: 909-916

    • NAID

      40021411922

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] BLV抵抗性・感受性の診断技術と新しい牛白血病制圧戦略 (特集 牛白血病ウイルス(BLV)に対する理研の挑戦)2017

    • Author(s)
      間 陽子, 竹嶋 伸之輔, 陸 拾七
    • Journal Title

      獣医畜産新報

      Volume: 70(12) Pages: 901-908

    • NAID

      40021411902

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] 若齢牛の地方病性牛白血病 (特集 牛の疾患 : 最近のトピックス)2017

    • Author(s)
      竹嶋伸之輔
    • Journal Title

      獣医畜産新報

      Volume: 70(9) Pages: 666-670

    • NAID

      40021319757

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] The first molecular epidemiological study of bovine leukemia virus infection in Myanmar cattle2017

    • Author(s)
      Meripet Polat, Hla Hla Moe, Takeshi Shimogiri, Kyaw Kyaw Moe, Shin-nosuke Takeshima, Yoko Aida
    • Journal Title

      Arch. Virol.

      Volume: 162(2) Issue: 2 Pages: 425-437

    • DOI

      10.1007/s00705-016-3118-y

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] 牛白血病対策の新技術に迫る(3)2017

    • Author(s)
      竹嶋伸之輔・綿貫園子・間陽子
    • Journal Title

      臨床獣医

      Volume: 1 Pages: 26-31

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] 牛白血病対策の新技術に迫る(1)2016

    • Author(s)
      竹嶋伸之輔・綿貫園子・間陽子
    • Journal Title

      臨床獣医

      Volume: 34 Pages: 34-40

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] 牛白血病対策の新技術に迫る(2)2016

    • Author(s)
      竹嶋伸之輔・綿貫園子・間陽子
    • Journal Title

      臨床獣医

      Volume: 12 Pages: 30-35

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] ホルスタイン種における牛白血病抵抗性・感受性を規定するウシ主要組織適合遺伝子複合体クラスIIアリルの同定2018

    • Author(s)
      竹嶋伸之輔・大野歩・間陽子
    • Organizer
      日本獣医師会
    • Related Report
      2017 Research-status Report
  • [Presentation] A Vaccine Targeting Bovine Leukemia Virus Susceptible Cattle Suppresses Proviral Load2017

    • Author(s)
      Yoko Aida, Lanlan Bai, Jiyun Kim, Pan He, Yuki Matsumoto, Noriaki Okimoto, Junya Yamagishi, Seiichi Tada, Yoshihiro Ito, Junko Kohara, Shin-nosuke Takeshima
    • Organizer
      第18回国際HTLV会議
    • Place of Presentation
      ホテルグランドアーク半蔵門(東京都千代田区)
    • Year and Date
      2017-03-07
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Molecular Genetic Analysis of Bovine Leukemia Virus (BLV) Worldwide ..2017

    • Author(s)
      M Polat, S Takeshima, A Ohno, T Miyasaka, J Kim, M K, Y Matsumoto, K Yamada, M Arainga, T Murakami, K Hosomichi, V de la Barra Diaz, CJ Panei, ET Gonzalez, G Giovambattista, M Kanemaki, C N Mingala, HH Moe, KK Moe, T Shimogiri, M Onuma, Y Aida
    • Organizer
      第18回国際HTLV会議
    • Place of Presentation
      ホテルグランドアーク半蔵門(東京都千代田区)
    • Year and Date
      2017-03-07
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] A novel CoCoMo Direct PCR Assay Detects Bovine Leukemia Virus from a Drop of Whole Blood2017

    • Author(s)
      Sonoko Watanuki, Shin-nosuke Takeshima, Yasunobu Matsumoto, Hiroshi Ishizaki, Kazuhiro Matoba, Yoko Aida
    • Organizer
      第18回国際HTLV会議
    • Place of Presentation
      ホテルグランドアーク半蔵門(東京都千代田区)
    • Year and Date
      2017-03-07
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Development of a Bovine Leukemia Virus-like Particle Vaccine and Its Immunogenecity in Mice and Cattle2017

    • Author(s)
      Hiroyuki Otsuki, Saeki Negishi, Lanlan Bai, Hirotaka Sato, Shin-nosuke Takeshima, Yoshitaka Imaizumi, Yasuko Nagai, Atsushi Iwamoto, Taichi Noro, Satoshi Sakamoto, Yuki Yamaguchi, Hiroshi Handa, Eiji Oishi, Yoko Aida
    • Organizer
      第18回国際HTLV会議
    • Place of Presentation
      ホテルグランドアーク半蔵門(東京都千代田区)
    • Year and Date
      2017-03-07
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Determination of Common B Cell Epitope in Bovine Leukemia Virus and Its Antibody Binding Sites2017

    • Author(s)
      Lanlan Bai, Hiroyuki Otsuki, Hirotaka Sato, Shin-nosuke Takeshima, Yoko Ai
    • Organizer
      第18回国際HTLV会議
    • Place of Presentation
      ホテルグランドアーク半蔵門(東京都千代田区)
    • Year and Date
      2017-03-07
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Chromosome 22 and Chromosome 23 Associated with the Development of BLV Infection Determined by a Genome-wide Association Study2017

    • Author(s)
      Shin-nosuke Takeshima, Shinji Sasaki, Polat Meripet, Yoshikazu Sugimoto, Yoko Aida
    • Organizer
      第18回国際HTLV会議
    • Place of Presentation
      ホテルグランドアーク半蔵門(東京都千代田区)
    • Year and Date
      2017-03-07
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] 全ゲノム相関解析による牛白血病ウイルスのプロウイルス量を制御する新規宿主因子 の同定2017

    • Author(s)
      竹嶋伸之輔、佐々木慎二、Polat Meripet、杉本喜一、間陽子
    • Organizer
      日本獣医学会
    • Related Report
      2017 Research-status Report
  • [Presentation] ゲノムワイド相関解析を用いた牛白血病ウイルスの 病態進行を制御する新規宿主遺伝子の同定2017

    • Author(s)
      竹嶋伸之輔、佐々木慎二、Polat Meripet、杉本喜一、間陽子
    • Organizer
      日本HTLV学会
    • Related Report
      2017 Research-status Report
  • [Presentation] 牛白血病ウイルスの病態進行を規定するプロウイルス量を制御する新規宿主因子の全ゲノム相関解析による同定2017

    • Author(s)
      竹嶋伸之輔、佐々木慎二、Polat Meripet、杉本喜一、間陽子
    • Organizer
      日本組織適合性学会
    • Related Report
      2017 Research-status Report
  • [Presentation] Chromosome 22 and chromosome 23 associated with the development of BLV infection determined by a genome-wide association study2017

    • Author(s)
      Shin-nosuke Takeshima, Shinji Sasaki,Polat Meripet, Yoshikazu Sugimoto, Yoko Aida
    • Organizer
      HTLV and Related Viruses
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] CNTN3 rs110616206, MHC class III rs29026690 and MHC class I rs17872126 associated with the development of BLV infection determined by a genome-wide association study2017

    • Author(s)
      Shin-nosuke Takeshima, Shinji Sasaki,Polat Meripet, Yoshikazu Sugimoto, Yoko Aida
    • Organizer
      International Workshop on Retroviral Pathogenesis
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Single nucleotide polymorphism in the bovine MHC region of Japanese black cattle are associated with bovine leukemia virus proviral load2017

    • Author(s)
      Shin-nosuke Takeshima, Shinji Sasaki,Polat Meripet, Yoshikazu Sugimoto, Yoko Aida
    • Organizer
      International society of animal genetics
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] 日本在来牛と黒毛和種のウシMHC領域のリシークエンスによる比較2016

    • Author(s)
      嶋伸之輔・細道一善・万年英之・国枝哲夫・印牧美佐生・下桐猛・間陽子
    • Organizer
      第25回日本組織適合性学会大会
    • Place of Presentation
      北海道大学学術交流会館(北海道札幌市)
    • Year and Date
      2016-10-22
    • Related Report
      2016 Research-status Report
  • [Presentation] 見島牛・口之島牛および黒毛和種のウシ主要組織適合遺伝子領域のリシークエンスによる比較解析2016

    • Author(s)
      嶋伸之輔・細道一善・万年英之・国枝哲夫・印牧美佐生・下桐猛・間陽子
    • Organizer
      日本動物遺伝育種学会第17回大会
    • Place of Presentation
      国立大学法人 名古屋大学大学院工学研究科ES総合館(名古屋市千種区不老町)
    • Related Report
      2016 Research-status Report
  • [Patent(Industrial Property Rights)] ウシ白血病ウイルス(BLV)プロウイルスロードの判定方法およびその利用2017

    • Inventor(s)
      間陽子・竹嶋伸之輔・佐々木慎二・杉本喜憲
    • Industrial Property Rights Holder
      理化学研究所
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2017-017882
    • Filing Date
      2017-02-02
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2020-03-30  

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