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Clarification of causes for aberrant semen of Japanese Black beef bulls by analyses of testicular and metabolic hormones and sperm binding proteins and development of its treatment

Research Project

Project/Area Number 16K08058
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Veterinary medical science
Research InstitutionOsaka Prefecture University

Principal Investigator

Kawate Noritoshi  大阪府立大学, 生命環境科学研究科, 教授 (80221901)

Co-Investigator(Kenkyū-buntansha) 稲葉 俊夫  大阪府立大学, 生命環境科学研究科, 客員教授 (00137241)
玉田 尋通  大阪府立大学, 生命環境科学研究科, 教授 (10155252)
坂瀬 充洋  兵庫県立農林水産技術総合センター, 北部農業技術センター, 課長 (70463396)
Research Collaborator Hannan M. A.  
Weerakoon W.W.P.N.  
Kohama Namiko  
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords黒毛和種雄ウシ / 性成熟精液不良 / 精巣ホルモン / 代謝ホルモン / 血液生化学 / 精子結合蛋白質 / 黒毛和種牛 / 精液異常 / 血中エストラジオール濃度 / 血中コルチゾール濃度 / 血中インスリン濃度 / 栄養状態 / 精漿ホルモン濃度 / 精漿 / 精子結合蛋白質BSP / 血中ホルモン濃度 / 黒毛和種雄牛 / 精液性状不良 / 精子結合BSP蛋白質
Outline of Final Research Achievements

Plasma testicular and metabolic hormones, serum biochemical parameters, seminal plasma sperm binding protein and scrotal circumference were compared between Japanese Black beef bulls with normal and abnormal semen surrounding puberty. As the results, reduced secretions of insulin-like peptide 3, inhibin insulin-like growth factor-I are associated with aberrant semen of the bulls surrounding puberty. On the other hand, decreases of scrotal circumference and testosterone secretion were minor in the bulls with abnormal semen. It is assumed that fresh semen abnormality may be related with higher serum magnesium and aspartate aminotransferase and that sperm morphological defect can be associated with increase of creatinine. There were no differences between bulls with abnormal and normal semen for sperm binding protein and hormonal concentrations.

Academic Significance and Societal Importance of the Research Achievements

本研究でえられた黒毛和種雄ウシの精液不良と血中精巣ホルモン・代謝ホルモン・血液生化学・精漿精子結合蛋白質との関連性に関する成果は、これまで不明であった肉用牛の精液不良の原因の一端を明らかにした。これらの研究成果は、雄ウシの生殖機能解明の学術的進展に貢献するととにもに、種雄候補牛の選定や飼養管理技術に新たな情報を提供する。すなわち、性成熟前に血中インスリン様ペプチド3、インヒビンおよびインスリン様成長因子-I濃度を測定して、それらが低値のものは種雄候補牛から除外することで、精液不良の発生を低下させることができ、黒毛和種牛の生産性向上に寄与することができると考えられる。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (31 results)

All 2019 2018 2017 2016 Other

All Int'l Joint Research (1 results) Journal Article (12 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 12 results,  Open Access: 3 results,  Acknowledgement Compliant: 4 results) Presentation (14 results) (of which Int'l Joint Research: 9 results,  Invited: 1 results) Remarks (4 results)

  • [Int'l Joint Research] Dept of Biochem and Mol Biology/Medical University of South Carolina(米国)

    • Related Report
      2016 Research-status Report
  • [Journal Article] Production of Japanese Black calves by the transfer of embryos developed from <i>in vitro-</i>fertilized oocytes derived by ovum pick up and matured in culture with the mitogen-activated protein kinase kinase inhibitor U01262019

    • Author(s)
      Sakagami N, Konda K, Hashimura S, Kawate N, Inaba T, Tamada H.
    • Journal Title

      Journal of Veterinary Medical Science

      Volume: 81 Issue: 3 Pages: 379-382

    • DOI

      10.1292/jvms.18-0460

    • NAID

      130007612681

    • ISSN
      0916-7250, 1347-7439
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Age related and seasonal changes of plasma concentrations of insulin-like peptide 3 and testosterone from birth to early-puberty in Thoroughbred male horses2019

    • Author(s)
      Hannan MA, Murase H, Sato F, Tsogtgerel M, Kawate N, Nambo Y.
    • Journal Title

      Theriogenology

      Volume: In press Pages: 212-217

    • DOI

      10.1016/j.theriogenology.2019.04.014

    • NAID

      120006644944

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Plasma IGF-I, INSL3, testosterone, inhibin concentrations and scrotal circumferences surrounding puberty in Japanese Black beef bulls with normal and abnormal semen2018

    • Author(s)
      Weerakoon WWPN, Sakase M, Kawate N, Hannan MA, Kohama N, Tamada H
    • Journal Title

      Theriogenology

      Volume: 114 Pages: 54-62

    • DOI

      10.1016/j.theriogenology.2018.03.006

    • Related Report
      2018 Annual Research Report 2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Relationships of plasma insulin-like peptide 3, testosterone, inhibin, and insulin-like growth factor-I concentrations with scrotal circumference and testicular weight in Japanese Black beef bull calves2018

    • Author(s)
      Sakase M, Kitagawa K, Kibushi M, Kawate N, Weerakoon WWPN, Hannan MA, Kohama N, Tamada H.
    • Journal Title

      Journal of Reproduction and Development

      Volume: 64 Issue: 5 Pages: 401-407

    • DOI

      10.1262/jrd.2018-034

    • NAID

      130007497815

    • ISSN
      0916-8818, 1348-4400
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] EFFECTS OF GONADOTROPIN-RELEASING HORMONE ANTAGONIST DEGARELIX ON MUSTH AND SERUM TESTOSTERONE CONCENTRATIONS IN ASIAN ELEPHANTS ( ELEPHAS MAXIMUS).2018

    • Author(s)
      Pathirana IN, Rajapaksa C, Kawate N, Wimalarathne A, Fonseka L, Weerakoon PN, Hannan MA, Alexander B, Pushpakumara A, Ariyaratne S, Tamada H.
    • Journal Title

      Journal of Zoo and Wildlife Medicine

      Volume: 49 Issue: 3 Pages: 779-783

    • DOI

      10.1638/2018-0019.1

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] LH and testosterone secretions in response to GnRH challenge in pubertal Japanese Black beef bulls with normal and abnormal semen2018

    • Author(s)
      Sakase M, Weerakoon WWPN, Hannan MA, Kohama N, Tamada H, Kawate N.
    • Journal Title

      Journal of Veterinary Medical Science

      Volume: 80 Issue: 12 Pages: 1829-1833

    • DOI

      10.1292/jvms.18-0151

    • NAID

      130007529086

    • ISSN
      0916-7250, 1347-7439
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Rescue of the fetal damage associated with high intrauterine pressure by 17β-estradiol injection in ovariectomized progesterone-treated pregnant mice2018

    • Author(s)
      Tadotsu D, Kawate N, Tamada H.
    • Journal Title

      Endocrine Journal

      Volume: 65 Issue: 12 Pages: 1219-1224

    • DOI

      10.1507/endocrj.EJ18-0302

    • NAID

      130007553242

    • ISSN
      0918-8959, 1348-4540
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of gonadotropin-releasing hormone antagonist degarelix on musth and serum testosterone concentrations in Asian elephants (Elephas Maximus)2018

    • Author(s)
      Pathirana IN, Rajapaksa RC, Kawate N, Wimalarathne A, Fonseka L, Weerakoon WWPN, Hannan MA, Alexander B, Pushpakumara A, Ariyaratne S, Tamada H
    • Journal Title

      Journal of Zoo and Wildlife Medicine

      Volume: 49

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Effects of long-acting GnRH antagonist, degarelix acetate, on plasma insulin-like peptide 3, testosterone and luteinizing hormone concentrations, and scrotal circumference in male goats.2017

    • Author(s)
      Hannan MA, Kawate N, Fukami Y, Weerakoon WW, Bullesbach EE, Inaba T, Tamada H.
    • Journal Title

      Theriogenology

      Volume: 88 Pages: 228-235

    • DOI

      10.1016/j.theriogenology.2016.09.032

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Changes of plasma concentrations of insulin-like peptide 3 and testosterone, and their association with scrotal circumference during pubertal development in male goats.2017

    • Author(s)
      Hannan MA, Kawate N, Fukami Y, Weerakoon WW, Bullesbach EE, Inaba T, Tamada H.
    • Journal Title

      Theriogenology

      Volume: 92 Pages: 51-56

    • DOI

      10.1016/j.theriogenology.2017.01.009

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Fetal gender prediction based on maternal plasma testosterone and insulin-like peptide 3 concentrations at midgestation and late gestation in cattle.2016

    • Author(s)
      Kibushi M, Kawate N, Kaminogo Y, Hannan MA, Weerakoon WW, Sakase M, Fukushima M, Seyama T, Inaba T, Tamada H.
    • Journal Title

      Theriogenology

      Volume: 86 Issue: 7 Pages: 1764-1773

    • DOI

      10.1016/j.theriogenology.2016.05.039

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Acute regulation of plasma insulin-like peptide 3 concentrations by luteinizing hormone in male goats.2016

    • Author(s)
      Hannan MA, Kawate N, Fukami Y, Pathirana IN, Bullesbach EE, Inaba T, Tamada H.
    • Journal Title

      Theriogenology

      Volume: 印刷中 Issue: 3 Pages: 749-756

    • DOI

      10.1016/j.theriogenology.2016.02.028

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] Scrotal circumference and plasma concentrations of testicular and metabolic hormones from pre-puberty to post-puberty in Japanese Black beef bulls with normal, abnormal fresh and low-fertility post-thaw semen2018

    • Author(s)
      Weerakoon WWPN, Sakase M, Hannan MA, Kohama N, Tamada H, Kawate N
    • Organizer
      The 30th World Buiatrics Congress 2018
    • Related Report
      2018 Annual Research Report 2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Relationship of plasma insulin-like peptide 3, testosterone and insulin-like growth factor I concentrations with scrotal circumferences and testicular weights in Japanese Black beef male calves2018

    • Author(s)
      Kitagawa K, Sakase M, Kibushi M, Weerakoon WWPN, Kohama N, Tamada H, Kawate N
    • Organizer
      The 30th World Buiatrics Congress 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Inhibitory effects of long-term repeated treatments of sustainable GnRH antagonist, degarelix acetate, on caprine testicular functions2018

    • Author(s)
      Kawate N, Kanuki R, Hannan MA, Weerakoon WWPN, Tamada H
    • Organizer
      The 30th World Buiatrics Congress 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] イヌ精巣におけるステロイド産生関連因子遺伝子発現量の性成熟と加齢に伴う変化2018

    • Author(s)
      小川依理子、川手憲俊、稲葉俊夫、玉田尋通
    • Organizer
      第161回日本獣医学会学術集会(獣医繁殖分科会)
    • Related Report
      2018 Annual Research Report
  • [Presentation] ウシ卵子の体外成熟培養におけるMAPKK 阻害剤(U0126)添加が卵子胚発生関連因子の遺伝子発現におよぼす影響2018

    • Author(s)
      青山桃子、坂上信忠、川手憲俊、玉田尋通
    • Organizer
      平成30年度獣医学術近畿地区学会(産業動物獣医学会)
    • Related Report
      2018 Annual Research Report
  • [Presentation] ウシ卵子成熟培養初期のMAPKK 阻害剤添加による卵子におけるグルタチオンの増加と活性酸素種の減少2018

    • Author(s)
      青山桃子、坂上信忠、川手憲俊、玉田尋通
    • Organizer
      平成30年度日本獣医師会獣医学術学会年次大会(地区学会長賞受賞講演)
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Relationships of plasma insulin-like peptide 3, testosterone, inhibin and insulin-like growth factor-I concentrations with scrotal circumferences and testicular weights in Japanese Black beef male calves2018

    • Author(s)
      Kitagawa K, Sakase M, Kibushi M, Weerakoon WWPN, Kohama N, Tamada H, Kawate N
    • Organizer
      The 30th World Buiatrics Congress 2018
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Inhibitory effects of long-term repeated treatments of sustainable GnRH antagonist, degarelix acetate, on caprine testicular functions2018

    • Author(s)
      Kawate N, Kanuki R, Hannan MA, Weerakoon WWPN, Tamada H, Kawate N
    • Organizer
      The 30th World Buiatrics Congress 2018
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Comparison of plasma insulin-like growth factor-I, insulin-like peptide 3, testosterone and inhibin concentrations around puberty in Japanese Black beef bulls between normal and abnormal semen2017

    • Author(s)
      Weerakoon WWPN, Kawate N, Hannan MA, Sakase M, Kohama N, Tamada H
    • Organizer
      4th World Congress of Reproductive Bioloty
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Changes of plasma concentrations of insulin-like peptide 3 and testosterone, and their association with scrotal circumference during pubertal development in male goats2017

    • Author(s)
      Kawate N, Hannan MA, Fukami Y, Weerakoon WWPN, Bullesback EE, Inaba T, Tamada H
    • Organizer
      4th World Congress of Reproductive Bioloty
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Effects of GnRH antagonist on musth and plasma testosterone concentrations in Asian male elephants2017

    • Author(s)
      Rajapaksa RC, Pathirana IN, 川手憲俊, Weerakoon WWPN, Wimalarathne HDA, Fonseka WTL, Hannan MA, Alexander PABD, Pushpakumara PGA, Ariyaratne HBS, 玉田尋通
    • Organizer
      第160回日本獣医学会学術集会
    • Related Report
      2017 Research-status Report
  • [Presentation] Effects of long-acting GnRH antagonist on plasma insulin-like peptide 3 and testosterone concentrations and scrotal circumference in male goats2016

    • Author(s)
      N Kawate, M A Hannan, Y Fukami, W W P N Weerakoon, E E Bullesbach, T Inaba, H Tamada
    • Organizer
      The 18th International Congress on Animal Reproduction
    • Place of Presentation
      ル・ビンチ(フランス・ツール)
    • Year and Date
      2016-06-26
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Acute regulation of plasma insulin-like peptide 3 concentrations by luteinizing hormone in male goats2016

    • Author(s)
      M A Hannan, N Kawate, Y Fukami, W W P N Weerakoon, E E Bullesbach, T Inaba, H Tamada
    • Organizer
      The 18th International Congress on Animal Reproduction
    • Place of Presentation
      ル・ビンチ(フランス・ツール)
    • Year and Date
      2016-06-26
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] 妊娠中期・後期の母牛血中テストステロンおよびインスリン様ペプチド3濃度 による胎子雌雄判別2016

    • Author(s)
      木伏雅彦、上之郷佑実、M.A.Hannan、W.W.P.N.Weerakoon、坂瀬充洋、福島護之、瀬山智博、稲葉俊夫、玉田尋通、川手憲俊
    • Organizer
      第159回日本獣医学会学術集会・繁殖分科会
    • Place of Presentation
      日本大学生物資源科学部(神奈川県藤沢市)
    • Related Report
      2016 Research-status Report
  • [Remarks] 大阪府立大学 獣医学類/専攻 獣医繁殖学教室

    • URL

      http://www.vet.osakafu-u.ac.jp/reprod/

    • Related Report
      2018 Annual Research Report
  • [Remarks] Osaka Prefecture University Lab of Theriogenology

    • URL

      http://www.vet.osakafu-u.ac.jp/english/reprod/

    • Related Report
      2018 Annual Research Report
  • [Remarks] 大阪府立大学獣医繁殖学教室

    • URL

      http://www.vet.osakafu-u.ac.jp/reprod/

    • Related Report
      2017 Research-status Report
  • [Remarks] 獣医繁殖学教室 Lab of Theriogenology

    • URL

      http://www.vet.osakafu-u.ac.jp/reprod/

    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2022-02-16  

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