| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Outline of Final Research Achievements |
Transmembrane channel like protein 1 (TMC1) is the mechanoelectrical transduction (MET) channel in hair cells of the inner ear. I found a phenomenon that the cytosolic N-terminal region of overexpressed mouse TMC1 (mTMC1) is localized in nuclei of a small population of HEK293 cells. This suggests that in some cells the N-terminal region of mTMC1 is cleaved and transported into nuclei. In this study, I revealed the positions of the cleavage of overexpressed mTMC1 and the involvement of Importin α in the mechanism of accumulation of the N-terminal region into nuclei. Moreover, overexpression of the N-terminal fragment of mTMC1 changed the expression levels of several genes. These findings suggest the possibility that TMC1 is cleaved and the produced N-terminal fragment regulates transcription in hair cells of the inner ear. However, further investigation is required in order to prove that the transcriptional regulation by mTMC1 happens in vivo.
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