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Role of contraction-dependent myokines on the immune system

Research Project

Project/Area Number 16K08077
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Integrative animal science
Research InstitutionToyo University

Principal Investigator

Nedachi Taku  東洋大学, 生命科学部, 教授 (50375200)

Co-Investigator(Kenkyū-buntansha) 川口 英夫  東洋大学, 生命科学部, 教授 (50416921)
加藤 和則  東洋大学, 理工学部, 教授 (60233780)
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords骨格筋 / 運動 / マイオカイン / 免疫 / 分泌タンパク質 / 収縮 / 運動因子 / 免疫制御
Outline of Final Research Achievements

Recently, it has been widely recognized that skeletal muscle is an endocrine organ, producing numerous types of secreted proteins referred to as myokines that potentially transduce signals to other tissues/organs. The main aim of the present study was to identify novel myokines by our established method, which is composed of mouse C2C12 myotubes and an electrical pulse stimulator. Our initial screening suggested CCL5 and CXCL10 as a candidate novel myokine whose expression was reduced by skeletal muscle cell contraction. We further confirmed this reduction by animal experiments. Furthermore, we also analyzed the intracellular signaling pathways that are responsible for contraction-dependent CCL5 and CXCL10 reduction. Together with the known physiological functions of CCL5 and CXCL10, our results suggest that the reduction of these myokines may be involved in exercise-dependent regulation of the immune system.

Academic Significance and Societal Importance of the Research Achievements

新規同定されたCCL5については、運動によって血中濃度が変動することが明らかとなったため、運動マーカーとして使用できる可能性が高い。また、人為的にCCL5あるいはCXCL10発現を制御することで、運動依存的な免疫制御の一部を代替できる可能性もあり、これらのマイオカイン自体あるいは産生制御機構をターゲットとした創薬など多くの応用研究の展開が可能になったと考えられる。さらに、本研究によって運動依存性マイオカインに関する研究ストラテジーが確立されたため、今後は運動依存的な他の生理変化にも本ストラテジーを適応できる。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (10 results)

All 2019 2018 2017 2016

All Journal Article (4 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 4 results,  Open Access: 2 results) Presentation (5 results) (of which Int'l Joint Research: 3 results,  Invited: 1 results) Book (1 results)

  • [Journal Article] Identification of CCL5/RANTES as a novel contraction-reducible myokine in mouse skeletal muscle.2018

    • Author(s)
      Ishiuchi Y, Sato H, Komatsu N, Kawaguchi H, Matsuwaki T, Yamanouchi K, Nishihara M, Nedachi T.
    • Journal Title

      Cytokine.

      Volume: 108 Pages: 17-23

    • DOI

      10.1016/j.cyto.2018.03.012

    • Related Report
      2018 Annual Research Report 2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Skeletal muscle cell contraction reduces a novel myokine, chemokine (C-X-C motif) ligand 10 (CXCL10): potential roles in exercise-regulated angiogenesis.2018

    • Author(s)
      Ishiuchi Y, Sato H, Tsujimura K, Kawaguchi H, Matsuwaki T, Yamanouchi K, Nishihara M, Nedachi T.
    • Journal Title

      Biosci Biotechnol Biochem.

      Volume: 82 Issue: 1 Pages: 97-105

    • DOI

      10.1080/09168451.2017.1411778

    • Related Report
      2018 Annual Research Report 2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Glucose deprivation regulates the progranulin-sortilin axis in PC12 cells2017

    • Author(s)
      Ken-ichi Kawashima, Yuri Ishiuchi, Miki Konnai, Saori Komatsu, Hitoshi Sato, Hideo Kawaguchi, Nobumitsu Miyanishi, Jerome Lamartine, Masugi Nishihara, Taku Nedachi
    • Journal Title

      FEBS open bio

      Volume: 7 Issue: 2 Pages: 149-159

    • DOI

      10.1002/2211-5463.12164

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Glucose deprivation attenuates sortilin levels in skeletal muscle cells2017

    • Author(s)
      Ariga M, Yoneyama Y, Fukushima T, Ishiuchi Y, Ishii T, Sato H, Hakuno F, Nedachi T, Takahashi S
    • Journal Title

      Endocrine Journal

      Volume: 64 Issue: 3 Pages: 255-268

    • DOI

      10.1507/endocrj.EJ16-0319

    • NAID

      130005529466

    • ISSN
      0918-8959, 1348-4540
    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Temperature-dependent secretome profiling in C2C12 myotubes: potential roles of CXCL10 reduction by heat-dependent stress2018

    • Author(s)
      Yuri Ishiuchi, Kei Sato, Natsuki Fujinuma, Marie Miegebielle, Taku Nedachi
    • Organizer
      2018 ASCB | EMBO Annual Meeting
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 運動によって制御される骨格筋分泌因子群~マイオカインと細胞外小胞~2018

    • Author(s)
      根建 拓
    • Organizer
      第1回 筋スマート社会コンソーシアム
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 運動依存的な新規マイオカインの分泌変化及び発現制御メカニズム2018

    • Author(s)
      石内友里、根建 拓
    • Organizer
      日本筋学会第4回学術集会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Identification of CXCL10 as contraction-reduced myokine in mouse skeletal muscle2016

    • Author(s)
      shiuchi Y, Sato H, Matsuwaki K, Yamanouchi K, Kawaguchi H, Nishihara M, Nedachi T.
    • Organizer
      the EMBO meeting
    • Place of Presentation
      Mannheim, Germany
    • Year and Date
      2016-09-10
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Mechanism of contraction-dependent CCL5 reduction in C2C12 myotubes2016

    • Author(s)
      Sato H, Ishiuchi Y, Kawaguchi H, Nedachi T.
    • Organizer
      the EMBO meeting
    • Place of Presentation
      Mannheim, Germany
    • Year and Date
      2016-09-10
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Book] 骨格筋研究を核とした筋スマート社会2019

    • Author(s)
      根建 拓、石内友里
    • Publisher
      シーエムシー・リサーチ
    • Related Report
      2018 Annual Research Report

URL: 

Published: 2016-04-21   Modified: 2020-03-30  

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