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Post-mating changes in host finding behavior and olfactory reception of Pieris rapae females

Research Project

Project/Area Number 16K08099
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Insect science
Research InstitutionHiroshima University

Principal Investigator

Omura Hisashi  広島大学, 統合生命科学研究科, 准教授 (60335635)

Co-Investigator(Kenkyū-buntansha) 藤井 毅  東京大学, 大学院農学生命科学研究科(農学部), 特任助教 (30730626)
Research Collaborator ISHIKAWA Yukio  
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords植食性昆虫 / モンシロチョウ / 寄主選択 / 植物揮発性成分 / 幼虫糞 / 触角感受性 / 交尾 / 嗅覚 / 寄主探索 / 忌避作用 / 発現変動遺伝子 / RNAシークエンス / 化学分析 / 昆虫 / 行動学 / 応用動物 / 生理学
Outline of Final Research Achievements

This study addressed the olfactory-guided host finding of Pieris rapae females and the post-mating changes in its behavior and olfactory reception. Mated females were more responsive to host plant volatiles than virgin females, in which the treatment of plant models with synthetic blends of cabbage leaf increased the number of landing on plant models by mated females. Since antennal responsiveness to host plant volatiles was similar between mated and virgin females, such behavioral enhancement of mated females might be attributed to post-mating changes in central nervous system involved in olfactory reception. Host-finding females showed weak repellency against larval frass odor although actual repellents could not be identified, in which virgin females were more sensitive than mated females. Quantitative RNA analyses revealed that female antennae possessed extremely low amounts of RNA and that 11 and 18 RNA genes overexpressed in female thorax and eggs, respectively, after mating.

Academic Significance and Societal Importance of the Research Achievements

昼行性のチョウは視覚的手がかりを利用して遠隔的な寄主探索をおこなうと考えられてきた。本研究では、母チョウが視覚のみならず嗅覚も利用することを実験的に証明し、寄主探索の手がかりとなる植物由来のカイロモンを同定するとともに、幼虫糞の匂いが弱いながらも雌を忌避することを見いだした。これらの知見は、アブラナ科の重要害虫であるモンシロチョウの行動を匂い物質で制御するための基礎情報であり、新規防除法開発のシーズになりうる。また、雌成虫の行動は交尾後ダイナミックに変化しており、行動生理学的な視点においても、交尾前の雌の防除および交尾の攪乱がより重要であることが示唆された。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (7 results)

All 2019 2018 2017 2016 Other

All Journal Article (2 results) (of which Peer Reviewed: 1 results) Presentation (4 results) (of which Int'l Joint Research: 2 results) Remarks (1 results)

  • [Journal Article] 交尾により母となったモンシロチョウの行動変化2018

    • Author(s)
      藤井 毅、大村 尚
    • Journal Title

      アグリバイオ

      Volume: 2(6) Pages: 561-565

    • NAID

      40021808306

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Effects of mating on host selection by female small white butterflies Pieris rapae (Lepidoptera: Pieridae)2018

    • Author(s)
      Itoh Yuki、Okumura Yukiko、Fujii Takeshi、Ishikawa Yukio、Omura Hisashi
    • Journal Title

      Journal of Comparative Physiology A

      Volume: 204 Issue: 2 Pages: 245-255

    • DOI

      10.1007/s00359-017-1237-x

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Presentation] Effect of dimethyl disulfide, present in larval frass odors, on host selection by female adults of Pieris rapae2019

    • Author(s)
      奥村裕紀子、玉井壮太、藤井 毅、石川幸男、大村 尚
    • Organizer
      34th Annual Meeting of the International Society of Chemical Ecology
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Involvement of larval frass volatiles in host selection by female adults of Pieris rapae2017

    • Author(s)
      Okumura Yukiko, Fujii Takeshi, Ishikawa Yukio, Omura Hisashi
    • Organizer
      The joint meeting of the 33rd annual meeting of the ISCE and the 9th meeting of the APACE
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] モンシロチョウ雌成虫の寄主探索行動と触角嗅覚応答の交尾後の変動2017

    • Author(s)
      伊藤優希・奥村裕紀子・藤井 毅・石川幸男・大村 尚
    • Organizer
      第61回日本応用動物昆虫学会大会
    • Place of Presentation
      東京農工大学小金井キャンパス
    • Related Report
      2016 Research-status Report
  • [Presentation] 交尾によって変化するモンシロチョウ雌成虫の寄主探索行動2016

    • Author(s)
      大村 尚・伊藤優希・奥村裕紀子・藤井 毅・石川幸男
    • Organizer
      平成28年度日本応用動物昆虫学会中国支部・日本昆虫学会中国支部合同例会
    • Place of Presentation
      鳥取大学広報センター
    • Related Report
      2016 Research-status Report
  • [Remarks] 母となったモンシロチョウは匂いも利用して産卵場所を探す。

    • URL

      http://home.hiroshima-u.ac.jp/~homura/LCE/feature.html

    • Related Report
      2018 Annual Research Report

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Published: 2016-04-21   Modified: 2020-03-30  

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