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Molecular and neurobiological dissection of the circadian clock and the photoperiodic time measurement system involved in photoperiodic responses of wasps and flies.

Research Project

Project/Area Number 16K08101
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Insect science
Research InstitutionOsaka City University

Principal Investigator

Goto Shinsuke  大阪市立大学, 大学院理学研究科, 教授 (70347483)

Co-Investigator(Renkei-kenkyūsha) SHIGA Sakiko  
TACHIBANA Shin-Ichiro  
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Keywords昆虫 / 休眠 / 概日時計 / 母性効果 / 日長測定 / 光周性 / 内分泌系 / キョウソヤドリコバチ / ナミニクバエ / 遺伝子
Outline of Final Research Achievements

Insects anticipate upcoming seasons by measuring day length (or night length) of a day. In the present study, we focused on two insect species, of which photoperiodic time measurement systems differ, to clarify the molecular and neurobiological mechanisms involved in photoperiodic responses. Although we were unable to clarify the location of the brain clock cells putatively involved in photoperiodic time measurement, we verified that biosynthesis of the juvenile hormone, that is synthesized in and secreted at the corpora allata, is under control of the photoperiodic time measurement system. In a fly, we clarified the exact location of clock cells in the brain and their oscillation patterns under distinct photoperiods. In addition, we clarified that distinct geographic variations in photoperiodic responses are based on the differences in the circadian clock systems governing photoperiodic time measurement.

Academic Significance and Societal Importance of the Research Achievements

生物が日長に反応する性質である光周性は温帯の多くの生物に見られ,これらの生物が季節に適応するために必須の機構である.本研究は,日長読み取り機構が異なると考えられる2種の昆虫を用いて,光周性に関わる分子神経機構を解明することを目的とした.その結果,上述の成果をあげることができた.これらの成果は,昆虫の光周性機構の解明において重要な知見をもたらすものである.その成果の一部はすでに原著論文,総説,解説文として公開されており,本分野に携わる世界中の多くの研究者に大きな影響を与えている.また,国民の知的好奇心を刺激すべく,得られた成果の内容を市民講座などを通して社会に還元している.

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (12 results)

All 2019 2018 2017 2016

All Journal Article (5 results) (of which Peer Reviewed: 4 results) Presentation (7 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Distinct Physiological Mechanisms Induce Latitudinal and Sexual Differences in the Photoperiodic Induction of Diapause in a Fly2019

    • Author(s)
      Yamaguchi Koki、Goto Shin G.
    • Journal Title

      Journal of Biological Rhythms

      Volume: 34 Issue: 3 Pages: 293-306

    • DOI

      10.1177/0748730419841931

    • NAID

      120006649318

    • URL

      https://ocu-omu.repo.nii.ac.jp/records/2019951

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cell cycle arrest in the jewel wasp Nasonia vitripennis in larval diapause2018

    • Author(s)
      Shimizu Yuta、Mukai Ayumu、Goto Shin G.
    • Journal Title

      Journal of Insect Physiology

      Volume: 106 Issue: 2 Pages: 147-152

    • DOI

      10.1016/j.jinsphys.2016.11.011

    • NAID

      120006626751

    • URL

      https://ocu-omu.repo.nii.ac.jp/records/2019989

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Photoperiodism, Insects2018

    • Author(s)
      Goto Shin G.、Matsumoto Keiji
    • Journal Title

      The Encyclopedia of Reproduction 2nd Edition, Volume VI: Comparative Reproduction

      Volume: - Pages: 420-425

    • DOI

      10.1016/b978-0-12-809633-8.20587-6

    • ISBN
      9780128151457
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Distribution of PERIOD-immunoreactive neurons and temporal change of the immunoreactivity under long-day and short-day conditions in the larval brain of the flesh fly Sarcophaga similis2017

    • Author(s)
      Yamamoto Mizuho、Shiga Sakiko、Goto Shin G.
    • Journal Title

      Chronobiology International

      Volume: 34 Issue: 6 Pages: 819-825

    • DOI

      10.1080/07420528.2017.1310736

    • NAID

      120006503241

    • URL

      https://ocu-omu.repo.nii.ac.jp/records/2019766

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] 昆虫の季節適応研究の現状と今後の展開2017

    • Author(s)
      後藤慎介
    • Journal Title

      昆虫と自然

      Volume: 700 Pages: 20-23

    • Related Report
      2017 Research-status Report
  • [Presentation] 都市環境におけるニクバエの光周性2018

    • Author(s)
      向井歩、山口幸紀、北村文、後藤慎介
    • Organizer
      第62回日本応用動物昆虫学会大会
    • Related Report
      2017 Research-status Report
  • [Presentation] イソフルランがキョウソヤドリコバチの概日時計に及ぼす影響2017

    • Author(s)
      向井歩,後藤慎介
    • Organizer
      第61回日本応用動物昆虫学会大会
    • Place of Presentation
      東京農工大学(東京都・小金井市)
    • Year and Date
      2017-03-27
    • Related Report
      2016 Research-status Report
  • [Presentation] ナミニクバエの光周性における臨界日長の地理系統差と光周測時機構2017

    • Author(s)
      山口幸紀,後藤慎介
    • Organizer
      第61回日本応用動物昆虫学会大会
    • Place of Presentation
      東京農工大学(東京都・小金井市)
    • Year and Date
      2017-03-27
    • Related Report
      2016 Research-status Report
  • [Presentation] 麻酔薬イソフルランがキョウソヤドリコバチの光周性に及ぼす影響2017

    • Author(s)
      向井歩、後藤慎介
    • Organizer
      第24回(2017年度)日本時間生物学会学術大会
    • Related Report
      2017 Research-status Report
  • [Presentation] キョウソヤドリコバチ幼虫休眠時の発育停止と細胞分裂停止2017

    • Author(s)
      向井歩、後藤慎介
    • Organizer
      日本昆虫学会第77回大会
    • Related Report
      2017 Research-status Report
  • [Presentation] ナミニクバエの光周性の雌雄差と地理系統差を生み出す生理機構2016

    • Author(s)
      山口幸紀,後藤慎介
    • Organizer
      第23回日本時間生物学会学術大会,
    • Place of Presentation
      名古屋大学(愛知県・名古屋市)
    • Year and Date
      2016-11-12
    • Related Report
      2016 Research-status Report
  • [Presentation] RNAi targeted to the circadian clock gene period disrupts photoperiodism of the jewel wasp, Nasonia vitripennis.2016

    • Author(s)
      Mukai, A., Goto, S.G.
    • Organizer
      International Congress of Entomology 2016
    • Place of Presentation
      Orlando (USA)
    • Year and Date
      2016-09-25
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research

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Published: 2016-04-21   Modified: 2020-03-30  

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