• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Endosymbiotic bacteria that affect host meiosis

Research Project

Project/Area Number 16K08106
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Insect science
Research InstitutionNational Agriculture and Food Research Organization

Principal Investigator

KAGEYAMA Daisuke  国立研究開発法人農業・食品産業技術総合研究機構, 生物機能利用研究部門, 上級研究員 (60401212)

Co-Investigator(Kenkyū-buntansha) 佐原 健  岩手大学, 農学部, 教授 (30241368)
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywordsボルバキア / 共生細菌 / 減数分裂 / マイオティックドライブ / 染色体 / 性決定
Outline of Final Research Achievements

Wolbachia is known to manipulate the insect hosts by various ways for their own benefit. Our study revealed a novel type of host manipulation by Wolbachia. Female Eurema mandarina butterflies infected with Wolbachia strain wFem produce all-female offspring by crossing with males. These offspring females, as well as their mothers, were revealed to have Z0 karyotype (sex chromosome constitution). Wolbachia was found to disrupt the maternal inheritance of Z chromosomes. Circumstantial evidence support the possibility that Wolbachia digests maternal Z chromosome during female meiosis. These results can lead to the better understanding of reproduction and endosymbiosis and basic contribution to future pest management strategies.

Academic Significance and Societal Importance of the Research Achievements

本研究により、今まで全く知られていなかった方法で微生物が昆虫の生殖システムを操作していることが明らかとなった。ボルバキアとよばれる細菌が、昆虫の性決定に影響を与えるだけでなく、性染色体の遺伝を妨げていることを示すことができた。さらにその仕組みとして、状況証拠をもとに、メスの減数分裂期に性染色体をまるごと消失させているという仮説を提示することができた。この成果は、発生学、進化生物学に新たな光を当てるだけでなく、生物の人工制御の可能性を予感させる内容であり、基礎的に重要なものである。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (19 results)

All 2019 2018 2017 Other

All Journal Article (5 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 5 results,  Open Access: 2 results) Presentation (10 results) Book (3 results) Remarks (1 results)

  • [Journal Article] Functional crosstalk across IMD and Toll pathways: insight into the evolution of incomplete immune cascades.2019

    • Author(s)
      Nishide, Y., Kageyama, D., Yokoi, K., Tanaka, H., Futahashi, R., Fukatsu T.
    • Journal Title

      Proceedings of the Royal Society B

      Volume: 286 Issue: 1897 Pages: 20182207-20182207

    • DOI

      10.1098/rspb.2018.2207

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Feminizing Wolbachia endosymbiont disrupts maternal sex chromosome inheritance in a butterfly species2017

    • Author(s)
      Kageyama D, Ohno M, Sasaki T, Yoshido A, Konagaya T, Jouraku A, Kuwazaki S, Kanamori H, Katayose Y, Narita S, Miyata M, Riegler M, Sahara K
    • Journal Title

      Evolution Letters

      Volume: 1 Issue: 5 Pages: 232-244

    • DOI

      10.1002/evl3.28

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Wolbachia infections of the butterfly Eurema mandarina interfere with embryonic development of the sawfly Athalia rosae2017

    • Author(s)
      Kageyama D, Wang C-H, Hatakeyama M
    • Journal Title

      Journal of Invertebrate Pathology

      Volume: 150 Pages: 76-81

    • DOI

      10.1016/j.jip.2017.08.003

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Wolbachia-induced meiotic drive and feminization is associated with an independent occurrence of selective mitochondrial sweep in a butterfly2017

    • Author(s)
      Miyata M, Konagaya T, Yukuhiro K, Nomura M, Kageyama D
    • Journal Title

      Biology Letters

      Volume: 13 Issue: 5 Pages: 20170153-20170153

    • DOI

      10.1098/rsbl.2017.0153

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Enzymatic activity necessary to restore the lethality due to Escherichia coli RNase E deficiency is distributed among bacteria lacking RNase E homologues2017

    • Author(s)
      Tamura M, Kageyama D, Honda N, Fujimoto H, Kato A
    • Journal Title

      PLoS ONE

      Volume: 12 Issue: 5 Pages: e0177915-e0177915

    • DOI

      10.1371/journal.pone.0177915

    • NAID

      120006628878

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 2種のキチョウ・2系統のWolbachia ~その感染史を紐解く~2018

    • Author(s)
      宮田真衣・野村昌史・陰山大輔
    • Organizer
      第62回日本応用動物昆虫学会[鹿児島大]
    • Related Report
      2018 Annual Research Report
  • [Presentation] FISH法を用いたボルバキア感染キタキチョウの性染色体構成の確定2018

    • Author(s)
      佐々木達史・ 陰山大輔・ 佐原健
    • Organizer
      東北昆虫学会第1回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 異なる生殖操作を起こす2系統のWolbachia(wCIとwFem)がキチョウ姉妹種の宿主ミトコンドリアの分化へ及ぼす影響について2017

    • Author(s)
      宮田真衣・野村昌史・陰山大輔
    • Organizer
      第61回日本応用動物昆虫学会
    • Place of Presentation
      東京農工大学(東京都小金井市)
    • Year and Date
      2017-03-28
    • Related Report
      2016 Research-status Report
  • [Presentation] 細胞内共生細菌Wolbachiaは核型ZOを持つキタキチョウにO卵しか作らせないように操作し、W染色体が担うはずのメス決定機構を肩代わりしている2017

    • Author(s)
      陰山大輔・佐原健
    • Organizer
      第61回日本応用動物昆虫学会
    • Place of Presentation
      東京農工大学(東京都小金井市)
    • Year and Date
      2017-03-28
    • Related Report
      2016 Research-status Report
  • [Presentation] 共生細菌ボルバキアは宿主の卵形成と性決定を2段階の協調したメカニズムで操作している2017

    • Author(s)
      陰山大輔・佐原健
    • Organizer
      日本蚕糸学会第87回大会
    • Place of Presentation
      農林水産技術会議事務局筑波産学連携支援センター(茨城県・つくば市)
    • Year and Date
      2017-03-21
    • Related Report
      2016 Research-status Report
  • [Presentation] 共生細菌ボルバキアが与える宿主ミトコンドリアへの進化的影響について~キチョウ姉妹種の例を中心に2017

    • Author(s)
      宮田真衣・野村昌史・陰山大輔
    • Organizer
      日本生物地理学会第72回年次大会
    • Related Report
      2017 Research-status Report 2016 Research-status Report
  • [Presentation] 共生細菌ボルバキアはどこまで巧妙に宿主を操るのか?キタキチョウで明らかになった新知見を中心に2017

    • Author(s)
      陰山大輔
    • Organizer
      日本生物地理学会第72回年次大会
    • Related Report
      2017 Research-status Report 2016 Research-status Report
  • [Presentation] 2系統のWolbachiaが2種のキチョウの宿主ミトコンドリアへ及ぼす影響2017

    • Author(s)
      田真衣・野村昌史・陰山大輔
    • Organizer
      日本進化学会第19回大会
    • Related Report
      2017 Research-status Report
  • [Presentation] 宿主をメス化するボルバキア共生細菌は母から子への性染色体の伝達を阻害する2017

    • Author(s)
      陰山大輔・大野瑞紀・佐々木達史・吉戸敦生・小長谷達郎・上樂明也・桑崎誠剛・金森裕之・片寄裕一・成田聡子・宮田真衣・Markus Riegler・佐原健
    • Organizer
      2017年度生命科学系学会合同年次大会(ConBio2017)
    • Related Report
      2017 Research-status Report
  • [Presentation] 2種のキチョウ・2系統のWolbachia ~その感染史を紐解く~2017

    • Author(s)
      宮田真衣・野村昌史・陰山大輔
    • Organizer
      第62回日本応用動物昆虫学会
    • Related Report
      2017 Research-status Report
  • [Book] Encyclopedia of Reproduction 2nd Edition2018

    • Author(s)
      Michael Skinner
    • Total Pages
      3868
    • Publisher
      Elsevier
    • Related Report
      2018 Annual Research Report
  • [Book] 動物学の百科事典2018

    • Author(s)
      公益社団法人日本動物学会
    • Total Pages
      800
    • Publisher
      丸善出版
    • ISBN
      4621303090
    • Related Report
      2018 Annual Research Report
  • [Book] Chemical Ecology of Insects: Applications and Associations with Plants and Microbes2018

    • Author(s)
      Jun Tabata
    • Total Pages
      304
    • Publisher
      CRC Press
    • Related Report
      2018 Annual Research Report
  • [Remarks] 昆虫を操作する微生物

    • URL

      https://sites.google.com/site/kageyama000jp/

    • Related Report
      2018 Annual Research Report 2017 Research-status Report

URL: 

Published: 2016-04-21   Modified: 2020-03-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi