• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Understanding of the molecular mechanism of chitinolysis by Paenibacillus sp. str. FPU-7 and the bacterial cell surface display system.

Research Project

Project/Area Number 16K08114
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Environmental agriculture(including landscape science)
Research InstitutionFukui Prefectural University

Principal Investigator

Itoh Takafumi  福井県立大学, 生物資源学部, 准教授 (10402827)

Research Collaborator HIBI Takao  福井県立大学, 生物資源学部, 教授 (00285181)
KIMOTO Hisashi  福井県立大学, 生物資源学部, 教授 (70283166)
FUJII Yutaka  福井大学, 医学部, 教授 (80211522)
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
KeywordsChitin biomass / Chitinase / Paenibacillus / Oligosaccharides / chitinase / structural biology / chitin oligosaccharides / バイオマス / 難分解性多糖の利用 / キチン / グラム陽性細菌 / キチナーゼ
Outline of Final Research Achievements

This study has been carried out to utilize the biomass chitin effectively and we have been obtained the following four results. (1) The surface-expressed chitinase, ChiW, of Paenibacillus sp. str. FPU-7 can effectively degrade chitin using multi-domains. (2) The mutant enzyme of ChiW with high transglycosylation activity was developed by site-directed mutagenesis. (3) The proteins related to the ChiW gene expression and the chitin oligosaccharides transport were found in the expressed proteins of P. FPU-7 by the mass spectrometry-based proteomics. (4) The expression system on the bacterial cell surface was developed and used to display some useful enzymes.

Academic Significance and Societal Importance of the Research Achievements

土壌細菌Paeibacillus sp. str. FPU-7は、酵素ChiWを利用して、カニ殻由来の固体状のキチンをも強力に細胞表層で分解する。今回、ChiWがどのようにキチンを分解しているかを明らかにした。他の難分解性高分子多糖も含めて、より効率的で環境負荷の少ない酵素による多糖分解法の確立に繋がることが期待される。また、P. FPU-7の細胞表層タンパク質提示機構によって、細菌に複雑な機能を持たせることができた。このように、「細菌P. FPU-7による難分解性キチン分解機構の解明とその応用」による成果は、低環境負荷型の機能性材料生産技術の確立に貢献する。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (10 results)

All 2018 2017 2016 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (8 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results) Remarks (1 results)

  • [Journal Article] Crystal Structure of Chitinase ChiW from Paenibacillus sp. str. FPU-7 Reveals a Novel Type of Bacterial Cell-Surface-Expressed Multi-Modular Enzyme Machinery2016

    • Author(s)
      Takafumi Itoh, Takao Hibi, Fumiko Suzuki, Ikumi Sugimoto, Akihiro Fujiwara, Koji Inaka, Hiroaki Tanaka, Kazunori Ohta, Yutaka Fujii, Akira Taketo, and Hisashi Kimoto
    • Journal Title

      PLOS ONE

      Volume: 11 Issue: 12 Pages: e0167310-e0167310

    • DOI

      10.1371/journal.pone.0167310

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] グラム陽性細菌 (Paenibacillus sp. str. FPU-7) のキチン分解機構の解析2018

    • Author(s)
      伊藤 貴文、日び 隆雄、藤井 豊、木元 久
    • Organizer
      日本生化学会 北陸支部第36回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Paenibacillus 属細菌由来アルギン酸リアーゼの立体構造解析2018

    • Author(s)
      中川 えみ、伊藤 貴文、木元 久、日び 隆雄
    • Organizer
      第11回 北陸合同バイオシンポジウム
    • Related Report
      2018 Annual Research Report
  • [Presentation] Structure and function of alginate lyase from Paenibacillus sp. str. FPU-72018

    • Author(s)
      Takafumi Itoh, Emi Nakagawa, Moe Yoda, Takao Hibi, Hisashi Kimoto
    • Organizer
      The Asian Crystallographic Association (AsCA 2018)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Paenibacillus属細菌由来アルギン酸リアーゼの機能解析2018

    • Author(s)
      伊藤 貴文、中川 えみ、要田 萌、木元 久、日ビ 隆雄
    • Organizer
      日本農芸化学会2018年度大会
    • Related Report
      2017 Research-status Report
  • [Presentation] Paenibacillus属細菌FPU-7株のキチン分解と取り込みの構造基盤2017

    • Author(s)
      伊藤 貴文, 仲市 あかり, 藤井 豊, 日び 隆雄, 木元 久
    • Organizer
      2017年度 生命科学系学会 合同年次大会 (ConBio2017)
    • Related Report
      2017 Research-status Report
  • [Presentation] Paenibacillus属細菌由来細胞表層キチナーゼChiWの立体構造2017

    • Author(s)
      伊藤 貴文、日ビ 隆雄、藤井 豊、武藤 明、木元 久
    • Organizer
      日本農芸化学会、2017年度大会
    • Place of Presentation
      京都女子大学 (京都府・京都市)
    • Related Report
      2016 Research-status Report
  • [Presentation] Structure and Function of Cell-Surface-Expressed Multi-Modular Chitinase ChiW of Paenibacillus sp. str. FPU-7.2016

    • Author(s)
      Itoh, T., Hibi, T., Fujii, Y., Taketo, A. and Kimoto, H.
    • Organizer
      14th conference of the Asian Crystallographic Association (AsCA 2016)
    • Place of Presentation
      ハノイ (ベトナム)
    • Year and Date
      2016-12-04
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] 細胞表層で働くグラム陽性細菌由来キチナーゼの機能と立体構造2016

    • Author(s)
      伊藤 貴文、日ビ 隆雄、木元 久
    • Organizer
      日本応用糖質科学会、第5回応用糖質フレッシュシンポジウム
    • Place of Presentation
      エフピコRiM7 階ものづくり交流館 (広島県・福山市)
    • Related Report
      2016 Research-status Report
    • Invited
  • [Remarks]

    • URL

      http://www.fpu.ac.jp/faculty_members/ito-t.html

    • Related Report
      2018 Annual Research Report

URL: 

Published: 2016-04-21   Modified: 2020-03-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi