Understanding of the molecular mechanism of chitinolysis by Paenibacillus sp. str. FPU-7 and the bacterial cell surface display system.
| Project/Area Number |
16K08114
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Environmental agriculture(including landscape science)
|
|---|
| Research Institution | Fukui Prefectural University |
|---|
Principal Investigator |
Itoh Takafumi 福井県立大学, 生物資源学部, 准教授 (10402827)
|
|---|
| Research Collaborator |
HIBI Takao 福井県立大学, 生物資源学部, 教授 (00285181)
KIMOTO Hisashi 福井県立大学, 生物資源学部, 教授 (70283166)
FUJII Yutaka 福井大学, 医学部, 教授 (80211522)
|
|---|
| Project Period (FY) |
2016-04-01 – 2019-03-31
|
| Project Status |
Completed (Fiscal Year 2018)
|
| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
|---|
| Keywords | Chitin biomass / Chitinase / Paenibacillus / Oligosaccharides / chitinase / structural biology / chitin oligosaccharides / バイオマス / 難分解性多糖の利用 / キチン / グラム陽性細菌 / キチナーゼ |
|---|
| Outline of Final Research Achievements |
This study has been carried out to utilize the biomass chitin effectively and we have been obtained the following four results. (1) The surface-expressed chitinase, ChiW, of Paenibacillus sp. str. FPU-7 can effectively degrade chitin using multi-domains. (2) The mutant enzyme of ChiW with high transglycosylation activity was developed by site-directed mutagenesis. (3) The proteins related to the ChiW gene expression and the chitin oligosaccharides transport were found in the expressed proteins of P. FPU-7 by the mass spectrometry-based proteomics. (4) The expression system on the bacterial cell surface was developed and used to display some useful enzymes.
|
| Academic Significance and Societal Importance of the Research Achievements |
土壌細菌Paeibacillus sp. str. FPU-7は、酵素ChiWを利用して、カニ殻由来の固体状のキチンをも強力に細胞表層で分解する。今回、ChiWがどのようにキチンを分解しているかを明らかにした。他の難分解性高分子多糖も含めて、より効率的で環境負荷の少ない酵素による多糖分解法の確立に繋がることが期待される。また、P. FPU-7の細胞表層タンパク質提示機構によって、細菌に複雑な機能を持たせることができた。このように、「細菌P. FPU-7による難分解性キチン分解機構の解明とその応用」による成果は、低環境負荷型の機能性材料生産技術の確立に貢献する。
|
Report
(4 results)
Research Products
(10 results)
-
[Journal Article] Crystal Structure of Chitinase ChiW from Paenibacillus sp. str. FPU-7 Reveals a Novel Type of Bacterial Cell-Surface-Expressed Multi-Modular Enzyme Machinery2016
Author(s)
Takafumi Itoh, Takao Hibi, Fumiko Suzuki, Ikumi Sugimoto, Akihiro Fujiwara, Koji Inaka, Hiroaki Tanaka, Kazunori Ohta, Yutaka Fujii, Akira Taketo, and Hisashi Kimoto
-
Journal Title
PLOS ONE
Volume: 11
Issue: 12
Pages: e0167310-e0167310
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
-
-
-
-
-
-
-
-
-
