| Project/Area Number |
16K08138
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2018: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
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| Keywords | 天然ゴム / ラテックス / パラゴムノキ / 乳管細胞 / イソプレノイド / 転写制御因子 / 発現制御 / イソプレノイト |
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| Outline of Final Research Achievements |
Since natural rubber (NR) is a non-fungible natural polymer for rubber industries, improvement of the NR yield from the Para rubber tree (Hevea brasiliensis) through the molecular breeding approach is desired. Although many proteins involved in the NR biosynthesis have been identified, transcriptional regulation mechanisms for them in latex (cytoplasm of laticifer cells) remain to be elucidated. In this study, transcription factors involved in the metabolic regulation of NR were screened by yeast one-hybrid screening and transcriptional profiling of genes in H. brasiliensis. As results, two transcription factors predominantly expressed in laticifers in the Para rubber tree were shown to interact with 5’ upstream regions of coding sequences of the NR biosynthesis-related genes. These transcription factors are expected to be applied for the improvement of NR yields.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究で同定された転写制御因子の過剰発現による、天然ゴム生合成関連遺伝子群の包括的な発現制御と天然ゴム生産性の向上が期待できる。また、本成果を応用することで、ラテックス特異的遺伝子発現システムの基盤構築も可能となる。このシステムにより、様々な有用二次代謝物生合成系をパラゴムノキで異種発現させることで、蓄積した目的代謝物をラテックスにとともに簡便に、かつ繰り返し回収可能である。主に熱帯地域で生育するパラゴムノキの機能改変による石油化学製品代替天然有機化合物の生産は、温室効果ガス低減と化石燃料枯渇対策を両立可能であり、世界の持続的発展の基盤となり得る。
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