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The origin of root nodule symbiotic organs in legumes

Research Project

Project/Area Number 16K08149
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Applied molecular and cellular biology
Research InstitutionNational Institute for Basic Biology

Principal Investigator

Soyano Takashi  基礎生物学研究所, 共生システム研究部門, 准教授 (60532819)

Project Period (FY) 2016-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords根粒共生 / 植物微生物相互作用 / 器官形成 / ミヤコグサ / 原基形成 / 植物 / 発生・分化 / 発現制御
Outline of Final Research Achievements

Legumes evolutionarily acquired the ability to produce root nodules as symbiotic organs in response to nitrogen-fixing bacteria, rhizobia. Organogenesis of root nodules is initiated by activation of a nodulation-specific NIN transcription factor. We revealed that a factor, LBD16-1, which is involved in early stages of lateral root development generally, was recruited into nodule organogenesis downstream NIN. LBD16-1 is directly targeted by NIN through an intronic cis-element conserved in leguminous orthologs. LBD16-1 positively regulates nodule primordia development together with NF-Y CAATA-box binding protein complex, of which subunit genes are also direct NIN targets.

Academic Significance and Societal Importance of the Research Achievements

皮層細胞に由来する根粒の進化学的起源については1980年代以前から議論がある。しかし、諸説ある中で確固たる結論は得られていなかった。本研究では、根粒形成過程において側根の発達プログラムの一部が転用されていることを証明した。本研究は植物科学における大きな疑問の一つに回答を与えるものである。さらに、植物で一般に保存される因子が根粒共生を制御する仕組みや共生経路とこのような因子との接点を明らかにすることによって、根粒共生を多様な植物に応用活用する試みにおける具体的な手法の確立に貢献できる。

Report

(5 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (14 results)

All 2019 2018 2017 2016 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (12 results) (of which Int'l Joint Research: 4 results,  Invited: 3 results) Remarks (1 results)

  • [Journal Article] A shared gene drives lateral root development and root nodule symbiosis pathways in Lotus2019

    • Author(s)
      Soyano Takashi、Shimoda Yoshikazu、Kawaguchi Masayoshi、Hayashi Makoto
    • Journal Title

      Science

      Volume: 366 Issue: 6468 Pages: 1021-1023

    • DOI

      10.1126/science.aax2153

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Presentation] Regulation of nodule development through factors involved in lateral root development2019

    • Author(s)
      Takashi Soyano, Makoto Hayashi, Masayoshi Kawaguchi
    • Organizer
      Asian Conference on Plant-Microbe Symbiosis and Nitrogen Fixation
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] マメ科植物の根粒原基と側根の形成は共通した因子によって制御される2019

    • Author(s)
      征矢野敬、下田宜司、川口正代司、林誠
    • Organizer
      植物微生物研究会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Co-option of factors involved in lateral root development into leguminous nodule organogenesis2019

    • Author(s)
      Takashi Soyano
    • Organizer
      International Congress on Nitrogen Fixation
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Root nodule symbiosis integrated into regulatory systems of host legumes2019

    • Author(s)
      Takashi Soyano
    • Organizer
      Imaging and Quantitative Biology
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] ミヤコグサASL18/LBD16はNF-Yと結合して根粒の発達を正に制御する2019

    • Author(s)
      征矢野敬、林誠、川口正代司
    • Organizer
      日本植物生理学会
    • Related Report
      2018 Research-status Report
  • [Presentation] Transcription factors involved in lateral root development may function downstream of NODULE INCEPTION to regulate nodule development2018

    • Author(s)
      Takashi Soyano, Makoto Hayashi, Masayoshi Kawaguchi
    • Organizer
      European Nitrogen Fixation Conference
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] マメ科植物の根粒と側根の形成に共通して関わる宿主因子2018

    • Author(s)
      征矢野敬、林誠、川口正代司
    • Organizer
      日本植物学会
    • Related Report
      2018 Research-status Report
  • [Presentation] 根粒共生特異的NIN転写因子の下流で作用する側根形成関連因子2018

    • Author(s)
      征矢野 敬、林 誠、川口正代司
    • Organizer
      日本植物生理学会
    • Related Report
      2017 Research-status Report
  • [Presentation] 根粒の発達に必須なミヤコグサ NN の下流 で作用する転写因子2017

    • Author(s)
      征矢野敬, 川口正代司, 林誠
    • Organizer
      日本植物生理学会
    • Place of Presentation
      鹿児島大学郡元キャンパス(鹿児島県鹿児島市)
    • Year and Date
      2017-03-16
    • Related Report
      2016 Research-status Report
  • [Presentation] 根粒の発達に必須なミヤコグサ NN の下流で作用する転写因子2017

    • Author(s)
      征矢野 敬、林 誠、川口正代司
    • Organizer
      日本植物生理学会
    • Related Report
      2017 Research-status Report
  • [Presentation] 根粒共生特異的転写因子 NIN の下流で作用する側根形成関連因子2017

    • Author(s)
      征矢野 敬、林 誠、川口正代司
    • Organizer
      植物微生物研究会
    • Related Report
      2017 Research-status Report
  • [Presentation] マメ科植物の根粒器官形成機構とその起源2016

    • Author(s)
      征矢野敬, 川口正代司, 林誠
    • Organizer
      日本植物学会
    • Place of Presentation
      沖縄コンベンションセンター(沖縄県宜野湾市)
    • Year and Date
      2016-09-16
    • Related Report
      2016 Research-status Report
    • Invited
  • [Remarks] マメ科植物の根粒と側根の発達は共通した遺伝子が制御することを発見

    • URL

      https://www.nibb.ac.jp/pressroom/news/2019/11/22.html

    • Related Report
      2019 Annual Research Report

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Published: 2016-04-21   Modified: 2021-02-19  

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