Refined structural analysis and mechanical property of drug nano-formulation by AFM in aqueous meida
| Project/Area Number |
16K08190
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Physical pharmacy
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| Research Institution | Chiba University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
東 顕二郎 千葉大学, 大学院薬学研究院, 准教授 (40451760)
植田 圭祐 千葉大学, 大学院薬学研究院, 助教 (40755972)
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 原子間力顕微鏡（AFM） / ナノ粒子 / 力学特性 / 構造評価 / 透過型電子顕微鏡（TEM） / 原子間力顕微鏡（AFM / ポリマー / NMR / 液中測定 / AFM / ナノ |
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| Outline of Final Research Achievements |
Preparation of liposome formulation and characterization: Effect of drug-loading conditions (amount/temperature/time) on morphology of doxorubicin (DOX)-loaded liposomes was characterized by AFM and cryoTEM. No only spherical but also prolate, oblate, and concave liposomes were formed with the enhanced DOX concentration. Not only liposomal morphologies in solution but also the difference of the mechanical properties were also evaluated by AFM force curve analysis
Preparation of drug nano-formulation and characterization: Effects of drug sturucture, crystal form, and the dispersed states in excipients on the drug nanoparticle formation were evaluated by using atomic force microscopy as well as the other physicochemical evaluation techniques.
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| Academic Significance and Societal Importance of the Research Achievements |
ナノ医薬品製剤の物性評価方法は、動的光散乱測定による平均粒子径・粒子径分布計測、SEMやTEMといった電子顕微鏡による形態観察が殆どである。後者は測定試料調製の際の乾燥や凍結により形態が変化しないナノ製剤には適用可能であるが、形態が変化したり崩壊したりする粒子も存在する。AFMによるナノ粒子の液中計測はリポソームが大半である。多くの場合、医薬品は未封入で構造や力学特性の評価も十分とはいえない。AFMを用いた液中計測による精密構造解析及び力学特性評価を各種ナノ医薬品製剤に展開することで、各種ナノ製剤毎の品質を確保する上で考慮すべき因子が明らかになる。
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Report
(4 results)
Research Products
(18 results)
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[Journal Article] Composition-dependent structural changes and antitumor activity of ASC-DP/DSPE-PEG nanoparticles.2016
Author(s)
Higashi, K., Mibu, F., Saito, K., Limwikrant, W., Yamamoto, K., Moribe, K.
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Journal Title
Eur. J. Pharm. Sci.
Volume: 99
Pages: 24-31
DOI
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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