| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
We have prepared phenylboronic acid-modified insulin (PBA-Ins) which has glucose-lowering effect and attempted to control its pharmacokinetics by utilizing the binding between PBA and sugar chains. As a model of sugar chains, poly(vinyl alcohol) (pVA) was used because it also binds with PBA. pVA was coated on the surface of an electrode of quartz crystal microbalance (QCM), and it was immersed in a solution of PBA-Ins. The QCM result confirmed adsorption of PBA-Ins on the pVA surface. In addition, a layer-by-layer (LbL) film composed of pVA and PBA-Ins was successfully prepared because there are two PBA moieties in one PBA-Ins molecules. By using the LbL film, we confirmed that the adsorbed PBA-Ins was released in the presence of fructose. These results show that PBA modified drugs have a potential to become a molecular machine whose destination can be controlled depending on the presence or absence of sugars. This study provides the working principle of the molecular machine.
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