| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
Both Asn and Asp residues in the proteins were transformed to D-isoAsp residues by repairing process of the repair enzyme PCMT and D-isoAsp residues were accumulated in aged proteins. In this study, we focused on D-isoAsp residues in proteins to study the relationship to the age-related diseases. Thus, we aimed to develop the useful tools for analyzing the D-isoAsp residues by using unique photochemical technology and gene engineering techniques. We performed the point mutation researches and molecular evolution studies using error-prone PCR technique and accomplished the basic researches for developing the new tool.
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