Development of a method for the analysis of protein O-GlcNAcylation using two-step click chemistry
| Project/Area Number |
16K08219
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Physical pharmacy
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| Research Institution | Tokyo Metropolitan Geriatric Hospital and Institute of Gerontology |
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Principal Investigator |
Tsumoto Hiroki 地方独立行政法人東京都健康長寿医療センター(東京都健康長寿医療センター研究所), 東京都健康長寿医療センター研究所, 研究員 (00409385)
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | O-GlcNAc / プロテオミクス / 質量分析 |
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| Outline of Final Research Achievements |
In this study, a method for the enrichment of O-GlcNAcylated peptides using two-step click chemistry was developed. Analysis of O-GlcNAcylated proteins was performed using extracts from rat kidney tissue. A total of 34 O-GlcNAcylated peptides from 28 proteins were identified. Of these, 2 proteins were increased and 7 proteins were decreased in diabetic rat.
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| Academic Significance and Societal Importance of the Research Achievements |
タンパク質のO-GlcNAc化は血糖値によって変化することから、糖尿病において重要な修飾である。本研究課題により、糖尿病モデル動物の腎臓で増加あるいは減少するO-GlcNAc化タンパク質の同定に成功した。今後、これらの生物学的な意義を明らかにすることで、糖尿病性腎症の病因・病態の解明に繋がる可能性がある。
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Report
(4 results)
Research Products
(5 results)
