Regulation of circadian clock by cell growth factor polyamines
| Project/Area Number |
16K08244
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | Chiba Institute of Science |
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Principal Investigator |
TERUI Yusuke 千葉科学大学, 薬学部, 教授 (60433687)
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | ポリアミン / 概日リズム / 体内時計 / BMAL1 / 遺伝子発現制御 / 時計遺伝子 / 翻訳 / 生体分子 / 細胞増殖 / ポリアミンモジュロン / Clock / p16 / 代謝リズム調節 / スペルミン |
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| Outline of Final Research Achievements |
We examined the relationship between polyamines and circadian rhythms.
The levels of polyamines showed a circadian rhythm. We next compared the expression level of 6 kinds of the circadian clock genes in control and DFMO (an inhibitor of polyamine synthesis) treated NIH3T3 cells. Although the mRNA level of clock genes did not alter in cells, the clock gene Bmal1 was increased about 2.5-fold at the protein level in control cells. The result suggests that Bmal1 synthesis is enhanced by polyamines at the level of translation. To clarify the mechanism of polyamine stimulation of Bmal1 synthesis, Bmal1-EGFP fusion plasmids were constructed. On the 5’-UTR of Bmal1 mRNA, there are two hairpin structures and complementary sequences to 18S rRNA necessary for ribosome shunting. Polyamine stimulation of Bmal1-EGFP synthesis disappeared by the deletion of these structures. These results suggest that polyamines enhance ribosome shunting and stimulate Bmal1 synthesis.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究により、BMAL1蛋白質のポリアミンによる合成促進機序とBMAL1の調節機構の生理的意義が明らかになり、体内時計制御の新たな経路を標的とした新規時差症候群回復薬や、様々な疾患に対する個々のリズム位相を知るための有益なバイオマーカーの開発の基盤が確立できると期待される。
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Report
(4 results)
Research Products
(43 results)
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[Journal Article] Poly-ion Complex of Chondroitin Sulfate and Spermine and Its Effect on Oral Chondroitin Sulfate Bioavailability2016
Author(s)
Ge D., Higashi K., Ito D., Nagano K., Ishikawa R., Terui Y., Higashi K., Moribe K., Linhardt RJ., Toida T.
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Journal Title
Chemical and Pharmaceutical Bulletin
Volume: 64
Issue: 5
Pages: 390-398
DOI
NAID
ISSN
0009-2363, 1347-5223
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Polyamines release the let-7b-mediated suppression of initiation codon recognition during the protein synthesis of EXT2.2016
Author(s)
Imamura M, Higashi K, Yamaguchi K, Asakura K, Furihata T, Terui Y, Satake T, Maegawa J, Yasumura K, Ibuki A, Akase T, Nishimura K, Kashiwagi K, Linhardt RJ, Igarashi K, Toida T
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Journal Title
Sci. Rep.
Volume: 6
Issue: 1
Pages: 33549-33549
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Presentation] Development of an ELISA for urinary metabolite of acrolein-glutathione conjugate.2018
Author(s)
Sakamoto, A., Terui, Y., Kashiwagi, K., Uemura, T., Igarashi, K.
Organizer
5th International Conference on Polyamines: Biochemical, Physiological and Clinical Perspectives.
Related Report
Int'l Joint Research
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[Presentation] Role of polyamines on cell growth under high temperature environment.2018
Author(s)
Terui, Y., Iwasaki, S., Sakamoto, A., Moriya, T., Oshima, T., Tamakoshi, M., Hori, H., Igarashi, K., Kashiwagi, K.
Organizer
5th International Conference on Polyamines: Biochemical, Physiological and Clinical Perspectives.
Related Report
Int'l Joint Research
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[Presentation] Effect of spermidine analogs on cell growth of Escherichia coli polyamine requiring mutant MA2612016
Author(s)
Yoshida T., Sakamoto A., Terui Y., Takao K., Sugita Y., Yamamoto K., Ishihama A., Igarashi K., Kashiwagi K.
Organizer
4th International Coference on Polyamines: Biochemical, Physiological and Clinical Perspectives
Place of Presentation
Rome, Italy
Year and Date
2016-09-04
Related Report
Int'l Joint Research
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