Identification of novel poly(ADP-ribosyl)ated proteins in spermatogonial stem cells
| Project/Area Number |
16K08257
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | Osaka Ohtani University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
黒川 優 大阪大谷大学, 薬学部, 助教 (70759761)
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 精子幹細胞 / ポリ(ADP-リボシル)化 / 神経幹細胞 / 発生生物学 / 再生医療 |
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| Outline of Final Research Achievements |
Poly(ADP-ribose) polymerase 1 (PARP1) has plays multiple roles in the cellular responses to DNA damage and the regulation of several nuclear events. Mouse spermatogonial stem cells (SSCs) and neural stem/progenitor cells (NSPCs) express higher levels of poly(ADP-ribose) polymerase 1 (PARP1) than mouse embryonic fibroblasts (MEFs). However, the molecular mechanisms involved in the regulation of PARP1 expression in tissue stem cells remain to be elucidated. In the present study, to identify the transcription factor involved in PARP1 transcription in mouse NSCPs, we performed a luciferase reporter assay and found two transcriptional regulatory elements upstream of the mammalian conserved promoter region.
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| Academic Significance and Societal Importance of the Research Achievements |
精子幹細胞や神経幹細胞では、通常の培養状態で体細胞に比べPARPが高発現し、常にNAD+を過剰に消費してポリ(ADP-リボシル)化（PAR化）が亢進状態である。このことから、体細胞にはない、これら幹細胞特有のPAR化の重要な働きがあることが予想される。本研究によって、組織幹細胞におけるPARP1の発現制御機構と、組織幹細胞においてPAR化が細胞周期関連タンパク質の発現を制御することを明らかにした。最近、PARP1阻害剤が抗がん剤として臨床応用されていることから、PAR化の多彩な役割を解明することによる学術的及び社会的意義は大きいと考えられる。
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Report
(4 results)
Research Products
(4 results)
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[Journal Article] Poly(ADP-ribose) Polymerase Inhibitors Activate the p53 Signaling Pathway in Neural Stem/Progenitor Cells.2017
Author(s)
Okuda, A., Kurokawa, S., Takehashi, M., Maeda, A., Fukuda, K., Kubo, Y., Nogusa, H., Takatani-Nakase, T., Okuda, S., Ueda, K., Tanaka, S.
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Journal Title
BMC Neurosci.
Volume: 18
Issue: 1
Pages: 14-14
DOI
Related Report
Peer Reviewed / Open Access
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