Rapid regulation of sialidase activity in response to neural activity and sialic acid removal during memory processing
| Project/Area Number |
16K08277
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pharmacology in pharmacy
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| Research Institution | University of Shizuoka |
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Principal Investigator |
Minami Akira 静岡県立大学, 薬学部, 講師 (80438192)
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| Research Collaborator |
Suzuki Takashi
Ikeda Kiyoshi
Kanazawa Hiroaki
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | シアリダーゼ / 神経 / 海馬 / 記憶 / 糖鎖 / 蛍光プローブ / シアル酸 / in vivo / シナプス可塑性 / 長期増強 / 神経糖鎖生物学 / グルタミン酸 / 脳梗塞 / ラット / マイクロダイアリシス / BTP3-Neu5Ac / 脳神経疾患 / 脳・神経 / 老化 |
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| Outline of Final Research Achievements |
We previously found that sialidase, which removes an acidic sugar molecule from the end of the sugar chain, rapidly changes enzyme activity in response to neural excitation on the cell surface and is essential for memory formation. If the sugar chain structure is regulated on the cell surface, it is possible to change the sugar chain structure instantaneously. In this study, based on the working hypothesis that “a rapid change in sugar chain structure in response to neural excitation controls neural circuit formation during memory formation”, the function of sialidase in memory was investigated by using a novel analysis tools for glycans. The present study revealed that sialic acid detaches during memory formation, and that this detachment is one of the negative feedback mechanisms for glutamate release and is related to the long-term potentiation that is the basic principle of memory.
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| Academic Significance and Societal Importance of the Research Achievements |
神経における糖鎖の機能解析は、タンパク質の機能解析などと比較して遅れている。その原因として、糖鎖機能の解析ツールが限られていることが挙げられる。記憶などの極めて短時間で変化する現象において糖鎖の役割を理解するためには、新たな糖鎖機能の解析ツールが必要である。本研究では研究代表者らが開発した新たな糖鎖機能分析ツールを利用することにより、神経興奮に伴って糖鎖構造が極めて短いタイムスケール（単位：秒や分）で厳密に制御されていることが明らかになった。この研究成果を基盤として、現在全く新しい視点から疾患や生理機能の機能解明に取り組んでいる
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Report
(4 results)
Research Products
(99 results)
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[Journal Article] Down-regulation of glutamate release from hippocampal neurons by sialidase2018
Author(s)
Akira Minami, Ami Ishii, Sumika Shimba, Takahiro Kano, Eri Fujioka, Saki Sai, Nagisa Oshio, Sayaka Ishibashi, Tadanobu Takahashi, Yuuki Kurebayashi, Hiroaki Kanazawa, Nobuhiro Yuki, Tadamune Otsubo, Kiyoshi Ikeda and Takashi Suzuki
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Journal Title
J. Biochem.
Volume: 163
Issue: 4
Pages: 273-280
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] Rapid regulation of sialidase activity in response to neural activity and sialic acid removal during memory processing in rat hippocampus.2017
Author(s)
Akira Minami, Yuko Meguro, Sayaka Ishibashi, Ami Ishii, Mako Shiratori, Saki Sai, Yuuki Horii, Hirotaka Shimizu, Hokuto Fukumoto, Sumika Shimba, Risa Taguchi, Tadanobu Takahashi, Tadamune Otsubo, Kiyoshi Ikeda and Takashi Suzuki.
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Journal Title
J. Biol. Chem.
Volume: 292
Issue: 14
Pages: 5645-5654
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Blood brain barrier permeability of (－)-epigallocatechin gallate, its proliferation-enhancing activity of human neuroblastoma SH-SY5Y cells, and its preventive effect on age-related cognitive dysfunction in mice.2017
Author(s)
Monira Pervin, Keiko Unno, Aimi Nakagawa, Yuu Takahashi, Kazuaki Iguchi, Hiroyuki Yamamoto, Minoru Hoshino, Aya Hara, Akiko Takagaki, Fumio Nanjo, Akira Minami, Shinjiro Imai, Yuriyuki Nakamura
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Journal Title
Biochem. Biophys. Rep.
Volume: 9
Pages: 180-186
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Substrate Specificity of Equine and Human Influenza A Virus Sialidase to Molecular Species of Sialic Acid2016
Author(s)
Tadanobu Takahashi, Saori Unuma, Sawako Kawagishi, Yuuki Kurebayashi, Maiko Takano, Hiroki Yoshino, Akira Minami, Takashi Yamanaka, Tadamune Otsubo, Kiyoshi Ikeda, Takashi Suzuki.
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Journal Title
Biological and Pharmaceutical Bulletin
Volume: 39
Issue: 10
Pages: 1728-1733
DOI
NAID
ISSN
0918-6158, 1347-5215
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] N-glycolylneuraminic acid on human epithelial cells prevents entry of influenza A viruses that possess N-glycolylneuraminic acid binding ability.2018
Author(s)
Tadanobu Takahashi, Maiko Takano, Yuuki Kurebayashi, Midori Masuda, Sawako Kawagishi, Masahiro Takaguchi, Takashi Yamanaka, Akira Minami, Tadamune Otsubo, Kiyoshi Ikeda, Takashi Suzuki
Organizer
Sialoglyco 2018
Related Report
Int'l Joint Research
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