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Alteration of harmonized relationship between P2X7 receptor- and zinc-mediated signaling in depressive disease

Research Project

Project/Area Number 16K08284
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Pharmacology in pharmacy
Research InstitutionKyoto Pharmaceutical University

Principal Investigator

Nagasawa Kazuki  京都薬科大学, 薬学部, 教授 (30228001)

Co-Investigator(Kenkyū-buntansha) 西田 健太朗  京都薬科大学, 薬学部, 講師 (20533805)
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
KeywordsP2X7受容体 / アストロサイト / 酸化ストレス / 亜鉛 / うつ病 / 亜鉛トランスポータ
Outline of Final Research Achievements

In this research project, we examined whether alteration of astrocyte functionality which plays important roles in development of depressive disease was resulted from altered signaling mediated by P2X7 receptor, an ATP receptor, and zinc, and mechanism underlying taste disorder developed in depression-model mice. We have demonstrated that oxidative stress, a causative factor for depressive disease, -induced astrocyte functionality was due, at least in part, to decreased functional expression induced by alteration in intracellular zinc signaling. In addition, it appears that decreased sweet taste sensitivity in depression model mice is caused by increased expression of sweet taste receptors in circumvallate papillae in their taste buds, and 16S rRNA metagenome analyses reveal compositional change in their gut flora.

Academic Significance and Societal Importance of the Research Achievements

本研究課題の遂行によって、うつ病モデルマウスにおける亜鉛・P2X7受容体を介したアストロサイトの機能変動機構及び味覚異常分子機構を解明すると共に、脳-腸連関不全における腸内細菌叢役割を解明するための研究基盤が形成できた。これら成果は、うつ病に対する革新的治療法開発のための分子基盤の構築に加えて、味覚障害に起因した栄養素バランスの破綻及び腸内細菌叢の変動の是正が、こころの健康の維持・増進に繋がるという“食育”に繋がる重要な科学的根拠を提示できることから、極めて大きな社会貢献が期待される。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (24 results)

All 2019 2018 2017 2016

All Journal Article (8 results) (of which Peer Reviewed: 8 results,  Open Access: 5 results,  Acknowledgement Compliant: 3 results) Presentation (16 results) (of which Invited: 1 results)

  • [Journal Article] Copper accumulation in the brain causes the elevation of oxidative stress and less anxious behavior in Ts1Cje mice, a model of Down syndrome.2019

    • Author(s)
      Ishihara K, Kawashita E, Shimizu R, Nagasawa K, Yasui H, Sago H, Yamakawa K, Akiba S.
    • Journal Title

      Free Radic Biol Med.

      Volume: 134 Pages: 248-259

    • DOI

      10.1016/j.freeradbiomed.2019.01.015

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Ergothioneine ameliorates oxaliplatin-induced peripheral neuropathy by decreasing neuronal accumulation of platinum in rat dorsal root ganglia2018

    • Author(s)
      Kentaro Nishida, Kazuya Takeuchi, Ayami Hosoda, Shohei Sugano, Eri Morisaki, Akihiro Ohishi, Kazuki Nagasawa
    • Journal Title

      Life Sciences

      Volume: 207 Pages: 516-524

    • DOI

      10.1016/j.lfs.2018.07.006

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] ATP metabolizing enzymes ENPP1, 2 and 3 are localized in sensory neurons of rat dorsal root ganglion2018

    • Author(s)
      Kentaro Nishida, Yuka Nomura, Kana Kawamori, Akihiro Ohishi, Kazuki Nagasawa
    • Journal Title

      European Journal of Histochemistry

      Volume: 62 Pages: 2877-2877

    • DOI

      10.4081/ejh.2018.2877

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Oxidative stress-induced increase of intracellular zinc in astrocytes decreases their functional expression of P2X7 receptors and engulfing activity2017

    • Author(s)
      Furuta Takahiro、Mukai Ayumi、Ohishi Akihiro、Nishida Kentaro、Nagasawa Kazuki
    • Journal Title

      Metallomics

      Volume: 9 Issue: 12 Pages: 1839-1851

    • DOI

      10.1039/c7mt00257b

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Inhibitory effect of divalent metal cations on zinc uptake via mouse Zrt-/Irt-like protein 8 (ZIP8)2017

    • Author(s)
      Koike et al.
    • Journal Title

      Life Sciences

      Volume: 173 Pages: 80-85

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Species Difference in Sensitivity of Human and Mouse P2X7 Receptors to Inhibitory Effects of Divalent Metal Cations2017

    • Author(s)
      Fujiwara et al.
    • Journal Title

      Biological and Pharmaceutical Bulletin

      Volume: 40 Issue: 3 Pages: 375-380

    • DOI

      10.1248/bpb.b16-00887

    • NAID

      130005398493

    • ISSN
      0918-6158, 1347-5215
    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Oxidative stress upregulates zinc uptake activity via Zrt/Irt-like protein 1 (ZIP1) in cultured mouse astrocytes2016

    • Author(s)
      Furuta et al.
    • Journal Title

      Life Sciences

      Volume: 151 Pages: 305-312

    • DOI

      10.1016/j.lfs.2016.03.025

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Expression level of P2X7 receptor is a determinant of ATP-induced death of mouse cultured neurons2016

    • Author(s)
      Ohishi et al.
    • Journal Title

      Neuroscience

      Volume: 319 Pages: 35-45

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Presentation] 社会敗北ストレス負荷うつ病モデルマウスにおける甘味嗜好性及び甘味受容体の発現の変化について2019

    • Author(s)
      濱野咲佳、山田由紀江、上村祐介、桑垣衣里奈、高畑祐香、吉本和佳、長澤一樹
    • Organizer
      日本薬学会第139年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Intracellular labile zinc level is a determinant of vulnerability of cultured astrocytes to oxidative stress2018

    • Author(s)
      Kazuki Nagasawa, Takahiro Furuta, Akihiro Ohishi
    • Organizer
      The Joint Congress of the 40th Annual Meeting of Japanese Society of Biological Psychiatry and the 61st Annual Meeting of the Japanese Society for Neurochemistry
    • Related Report
      2018 Annual Research Report
  • [Presentation] 社会敗北ストレス負荷によるうつ様行動誘発におけるマウス系統差に関する研究2018

    • Author(s)
      小林真実、山田由紀江、上村祐介、桑垣衣里奈、濱野咲佳、大石晃弘、長澤一樹
    • Organizer
      日本味と匂学会第52回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 社会敗北ストレス負荷されたうつ病モデルマウスにおける甘味嗜好性及び甘味受容体の発現の変化2018

    • Author(s)
      山田由紀江、上村祐介、桑垣衣里奈、濱野咲佳、大石晃弘、長澤一樹
    • Organizer
      日本味と匂学会第52回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] TRPM7を介したアストロサイト細胞内遊離型亜鉛レベルの制御は酸化ストレスに対する脆弱性を決定する要因の一つである2018

    • Author(s)
      長澤一樹、古田能裕、大石晃弘
    • Organizer
      フォーラム2018
    • Related Report
      2018 Annual Research Report
  • [Presentation] Dextran sulfate sodium誘発性炎症性腸疾患に対するmagnesium経口投与の予防効果2018

    • Author(s)
      大堀健史、藤原麻紀子、大石晃弘、西田健太朗、魚住嘉伸、長澤一樹
    • Organizer
      海洋深層水利用学会第22回全国大会(韓国大会)
    • Related Report
      2018 Annual Research Report
  • [Presentation] 酸化ストレスに対するマウスアストロサイトの脆弱性の系統差は細胞内遊離型亜鉛レベルの差異に起因する2018

    • Author(s)
      古田能裕ら
    • Organizer
      日本薬学会第138年会
    • Related Report
      2017 Research-status Report
  • [Presentation] Dextran sulfate sodium (DSS) 誘発炎症性腸疾患に対するマグネシウム投与の影響について2017

    • Author(s)
      本田梨沙ら
    • Organizer
      日本薬学会第137年会
    • Place of Presentation
      仙台
    • Year and Date
      2017-03-24
    • Related Report
      2016 Research-status Report
  • [Presentation] 二価金属カチオンによるP2X7受容体の活性化に対する阻害効果のヒト及びマウス間での比較2017

    • Author(s)
      小林真実ら
    • Organizer
      日本薬学会第137年会
    • Place of Presentation
      仙台
    • Year and Date
      2017-03-24
    • Related Report
      2016 Research-status Report
  • [Presentation] 亜鉛によるグリア細胞機能制御2017

    • Author(s)
      長澤一樹
    • Organizer
      日本薬学会第137年会
    • Place of Presentation
      仙台
    • Year and Date
      2017-03-24
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] 酸化ストレス負荷により増加したアストロサイト細胞内遊離型亜鉛はP2X7受容体の機能的発現低下を介してその貪食活性を低下させる2017

    • Author(s)
      古田能裕ら
    • Organizer
      メタルバイオサイエンス研究会2017
    • Related Report
      2017 Research-status Report
  • [Presentation] Dextran sulfate sodium (DSS) 誘発性炎症性腸疾患に対するmagnesium経口投与の予防効果2017

    • Author(s)
      大堀健史ら
    • Organizer
      メタルバイオサイエンス研究会2017
    • Related Report
      2017 Research-status Report
  • [Presentation] Dextran sulfate sodium (DSS) 誘発炎症性腸疾患に対するmagnesium経口投与の予防効果2017

    • Author(s)
      大石晃弘ら
    • Organizer
      第11回次世代を担う若手医療薬科学シンポジウム
    • Related Report
      2017 Research-status Report
  • [Presentation] ATP誘発性神経細胞死に対するP2X7受容体の発現量の影響2016

    • Author(s)
      大石晃弘ら
    • Organizer
      第59回日本神経化学会大会・第38回日本生物学的精神医学会合同年会
    • Place of Presentation
      福岡
    • Year and Date
      2016-09-09
    • Related Report
      2016 Research-status Report
  • [Presentation] 酸化ストレス負荷によりアストロサイトにおけるP2X7受容体の機能的発現は減少する2016

    • Author(s)
      古田能裕ら
    • Organizer
      第59回日本神経化学会大会・第38回日本生物学的精神医学会合同年会
    • Place of Presentation
      福岡
    • Year and Date
      2016-09-09
    • Related Report
      2016 Research-status Report
  • [Presentation] Dextran sulfate sodium (DSS) 誘発炎症性腸疾患に対する二価金属カオチン投与の影響について2016

    • Author(s)
      大堀健史ら
    • Organizer
      第66回日本薬学会近畿支部総会・大会
    • Place of Presentation
      大阪
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2020-03-30  

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