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Development of origin identification method for animal crude drugs using molecular biological techniques

Research Project

Project/Area Number 16K08304
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Natural medicines
Research InstitutionJuntendo University

Principal Investigator

NAKANISHI Hiroaki  順天堂大学, 医学部, 准教授 (90392274)

Research Collaborator SAITO Kazuyuki  
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2016: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Keywords動物由来生薬 / 基原 / 鑑定 / ミトコンドリアDNA / DNA鑑定 / 12S rRNA / 16S rRNA / COI / 動物基原生薬 / 16SrRNA / 薬学 / 天然資源系薬学 / 漢方・和漢薬
Outline of Final Research Achievements

We developed an origin identification method using DNA analysis to improve quality control for crude drugs derived from animals. Thirty three samples in 24 kinds of crude drugs derived from animals were examined. DNA was extracted from almost all of the crude drugs by adjustment of commercially DNA extraction kit (QIAamp DNA Mini Kit). The species of origin were identified by BLAST analysis after sequencing the mitochondrial 16S rRNA, 12S rRNA and cytochrome oxidase I region. The origins were identified in all kinds of crude drugs except Longgu that DNA was not extracted. Our method proved very useful for identifying of the origins of crude drugs derived from animals.

Academic Significance and Societal Importance of the Research Achievements

本研究の成果は、従来の官能試験法、形態学的方法、化学的方法に続く方法として、生薬の品質管理に大いに役立つことが期待される。この方法は、客観的に動物種を判別できるだけでなく、粉末状になっても検査ができ、熟練度も不要である。学術面においては、様々な状態からのDNA抽出法を提示することで、薬学のみならず、法医学や食品学などの遺伝子研究に役立つものと考えられる。また、ワシントン条約等、捕獲に規制がある動物かどうかも判明できることから、食品偽装問題の解決等、社会的にも貢献できることが期待される。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (4 results)

All 2019 2018 2017

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (3 results)

  • [Journal Article] Establishment of widely applicable DNA extraction methods to identify the origins of crude drugs derived from animals using molecular techniques2018

    • Author(s)
      Nakanishi Hiroaki、Yoneyama Katsumi、Hayashizaki Yoshie、Hara Masaaki、Takada Aya、Saito Kazuyuki
    • Journal Title

      Journal of Natural Medicines

      Volume: 73 Issue: 1 Pages: 173-178

    • DOI

      10.1007/s11418-018-1261-3

    • NAID

      40021790144

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Presentation] 分子生物学的手法を用いた節足および環形動物由来生薬の基原動物鑑定2019

    • Author(s)
      中西 宏明, 米山 克美, 原 正昭, 髙田 綾, 齋藤 一之
    • Organizer
      日本薬学会第139年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 分子生物学的手法を用いた動物由来生薬の基原動物鑑定法の構築2018

    • Author(s)
      中西宏明、米山克美、林崎義映、原正昭、高田綾、齋藤一之
    • Organizer
      日本薬学会第138年会
    • Related Report
      2017 Research-status Report
  • [Presentation] 医史学的資料の分析~江戸期の医師井上貫流の遺した異物資料の成分検査~2017

    • Author(s)
      大森毅, 大津留修, 中西宏明, 笠松正昭, 杉田律子, 長岡功, 齋藤一之
    • Organizer
      日本薬学会第137年会
    • Place of Presentation
      仙台国際センター(宮城県仙台市)
    • Year and Date
      2017-03-24
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2020-03-30  

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