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Study of lipsomal vaccine entrapping MHC-binding peptides for production of secretory IgA against Shiga-like toxin B subunit

Research Project

Project/Area Number 16K08344
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Environmental and hygienic pharmacy
Research InstitutionUniversity of Shizuoka

Principal Investigator

KUROHANE KOHTA  静岡県立大学, 薬学部, 講師 (90333525)

Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
KeywordsIgA / ワクチン / 免疫学 / リポソーム / ベロ毒素 / 粘膜免疫 / 微生物 / 感染症
Outline of Final Research Achievements

B-subunit of Shiga-like toxin (Stx1B) has been demonstrated to be a poor immunogenicity. To augments the immunogenicity of Stx1B, major histocompatibility complex class II bind peptides, including T cell epitopes, and Stx1B were liposomalized. Mice were given Stx1B-liposomes via nasal cavity. As a result, lipsomalization of Stx1B efficiently augmented the immunogenicity of Stx1B in the mucosal immune systems. Moreover, secretory IgA (SIgA) genes were obtained from anti-Stx1B SIgA producing hybridoma. These genes were transfected into CHO-K1 cells to obtain anti-Stx1B SIgA in vitro. It was able to obtain anti-Stx1B SIgA has neutralizing activity.

Academic Significance and Societal Importance of the Research Achievements

粘膜免疫の効果的な誘導、抗原特異的IgA 抗体産生ハイブリドーマの作製、抗体を構成する遺伝子を用いて分泌型IgAを構築することが可能になれば、粘膜に投与可能な治療用抗体としての分泌型IgAの研究が大きく発展する。本研究では、病原細菌由来の毒素を抗原として用いることで、臨床応用可能な治療用抗体の作製が可能となる点にも特徴がある。本研究の概念を応用すれば、抗原性に関わらず、粘膜免疫を効果的に賦活化できる経粘膜ワクチンの開発、粘膜に適用可能な治療用分泌型IgAの開発につながり、感染症予防に大きく寄与すると思われる。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (5 results)

All 2018 2017

All Presentation (5 results)

  • [Presentation] TRPチャネル活性化物質によるFITC特異的抗体産生誘導2018

    • Author(s)
      黒羽子孝太、今井康之
    • Organizer
      USフォーラム2018
    • Related Report
      2018 Annual Research Report
  • [Presentation] 分泌型IgAのペプシン抵抗性:植物由来組換え型分泌片を用いた研究2018

    • Author(s)
      菊地祐希、松田弥奈美、森兼捷太、中西勝宏、黒羽子孝太、丹羽康夫、小林裕和、今井康之
    • Organizer
      日本病院薬剤師東海ブロック・日本薬学会東海支部合同学術大会2018
    • Related Report
      2018 Annual Research Report
  • [Presentation] FITC誘導接触性皮膚炎マウスモデルにおけるFITC特異的抗体産生2018

    • Author(s)
      黒羽子孝太、遠藤由貴奈、今井康之
    • Organizer
      日本薬学会第138年会
    • Related Report
      2017 Research-status Report
  • [Presentation] Transient receptor potential (TRP) チャネル活性化を指標としたアジュバント物質の探索2017

    • Author(s)
      黒羽子孝太
    • Organizer
      USフォーラム2017
    • Place of Presentation
      静岡
    • Year and Date
      2017-04-20
    • Related Report
      2016 Research-status Report
  • [Presentation] FITCを用いた経皮免疫による抗FITC抗体の誘導2017

    • Author(s)
      遠藤由貴奈、黒羽子孝太、今井康之
    • Organizer
      日本病院薬剤師会東海ブロック・日本薬学会東海支部合同学術大会2017
    • Related Report
      2017 Research-status Report

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Published: 2016-04-21   Modified: 2020-03-30  

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