| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
B-subunit of Shiga-like toxin (Stx1B) has been demonstrated to be a poor immunogenicity. To augments the immunogenicity of Stx1B, major histocompatibility complex class II bind peptides, including T cell epitopes, and Stx1B were liposomalized. Mice were given Stx1B-liposomes via nasal cavity. As a result, lipsomalization of Stx1B efficiently augmented the immunogenicity of Stx1B in the mucosal immune systems. Moreover, secretory IgA (SIgA) genes were obtained from anti-Stx1B SIgA producing hybridoma. These genes were transfected into CHO-K1 cells to obtain anti-Stx1B SIgA in vitro. It was able to obtain anti-Stx1B SIgA has neutralizing activity.
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