| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
M strains that form a large cluster of Mycobacterium tuberculosis isolated in Japan are one of the Beijing modern-type strains that are reported to be highly pathogenic. The whole genome analysis of the M strain revealed that 45 genes have a single nucleotide polymorphism (SNP) accompanied by amino acid substitution (non-synonymous).
Focusing on these genes, plasmids were constructed having the wild type nucleotide (H37Rv sequence) and the mutation type (M strain sequence). The transformation efficiency and the growth rate were compared between them. The fatty acid metabolic enzymes, fadD25 and fadB3 may be involved in infectivity and virulence because the transformation efficiency was higher when the M strain mutant type was expressed.
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