| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
|---|
| Outline of Final Research Achievements |
In this study, we aimed to analyze the role of xenobiotic metabolizing enzymes in immunotoxic response in skin by skin-sensitizers. As a result of transfecting various reporter genes into human skin keratinocyte HaCaT cells and evaluating its usefulness, it was shown that HaCaT-Nrf2-Luc cells as a useful cell line for analysis of skin immunotoxicity. The role of xenobiotic metabolizing enzymes in immunotoxicity by skin-sensitizers using this cell line were examined. As a result, it was suggested that the immunotoxicity of some skin-sensitizers are changed by activation/detoxification of chemicals by P450 enzymes expressed in HaCaT cells.
|
