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Skin microbiome transplantation to patients with atopic dermatitis; in vitro study

Research Project

Project/Area Number 16K08384
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Medical pharmacy
Research InstitutionMeiji Pharmaceutical University

Principal Investigator

Sugita Takashi  明治薬科大学, 薬学部, 教授 (10312076)

Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsアトピー性皮膚炎 / マイクロバイオーム / 個別化治療 / 皮膚 / 微生物
Outline of Final Research Achievements

Atopic dermatitis is a disease where there are repeated exacerbation and remission, and skin microbiome can also be changed during exacerbation and remission periods. Fungus Malassezia is one of the exacerbating factors of atopic dermatitis. In this study, we have succeeded in isolating Staphylococcus sp. MPU-7 from patients as a bacterium with the ability to reduce cell cytotoxicity. The microorganism was also able to increase the expression of skin barrier function related proteins and antimicrobial peptides in vitro. There are considerations for strain MPU7-1 to be a candidate for skin microbiome transplantation.

Academic Significance and Societal Importance of the Research Achievements

アトピー性皮膚炎の小児期から思春期の我が国における有病率は10%にも達するが年齢とともにその率は減少する。しかしその一部は成人になっても寛解せずに難治化する場合がある。近年、ヒトのマイクロバイオームは多くの疾患に関与することが明らかになり、潰瘍性大腸炎等には糞便を移植するマイクロバイオーム治療が臨床の場に供されている。本研究は、対象療法が主たる治療法となるアトピー性皮膚炎について、その患者の皮膚環境をマイクロバイオームを移植することにより治療しようとする新しい発想である。そのための候補株を選定することに成功した。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (36 results)

All 2019 2018 2017 2016

All Journal Article (8 results) (of which Peer Reviewed: 7 results,  Open Access: 2 results,  Acknowledgement Compliant: 2 results) Presentation (27 results) (of which Int'l Joint Research: 3 results,  Invited: 10 results) Book (1 results)

  • [Journal Article] Characterization of the Axillary Microbiota of Japanese Male Subjects with Spicy and Milky Odor Types by Pyrosequencing2018

    • Author(s)
      Okamoto H, Koizumi S, Shimizu H, Cho O, Sugita T
    • Journal Title

      Biocontrol Science

      Volume: 23 Issue: 1 Pages: 1-5

    • DOI

      10.4265/bio.23.1

    • NAID

      130006539133

    • ISSN
      1342-4815, 1884-0205
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Culture Supernatants of Lactobacillus gasseri and L. crispatus Inhibit Candida albicans Biofilm Formation and Adhesion to HeLa Cells.2018

    • Author(s)
      Matsuda Y, Cho O, Sugita T, Ogishima D, Takeda S
    • Journal Title

      Mycopathologia

      Volume: 印刷中 Issue: 4 Pages: 691-700

    • DOI

      10.1007/s11046-018-0259-4

    • Related Report
      2018 Annual Research Report 2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Loop-mediated isothermal amplification for the rapid detection of Gardnerella vaginalis.2018

    • Author(s)
      Higashide S, Cho O, Matsuda Y, Ogishima D, Kurakado S, Sugita T
    • Journal Title

      Microbiol. Immunol

      Volume: 62 Pages: 607-611

    • NAID

      40021669911

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Inhibitory effects of Euphorbia supina on Propionibacterium acnes-induced skin inflammation in vitro and in vivo2018

    • Author(s)
      Lim HJ, Jeon YD, Kang SH, Shin MK, Lee KM, Jung SE, Cha JY, Lee HY, Kim BR, Hwang SW, Lee JH, Sugita T, Cho O, Myung H, Jin JS, Lee YM
    • Journal Title

      BMC Complement Altern. Med

      Volume: 18 Issue: 1 Pages: 263-263

    • DOI

      10.1186/s12906-018-2320-8

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] マラセチアとマラセチア皮膚炎の発症機序2018

    • Author(s)
      杉田 隆
    • Journal Title

      Monthly Book Derma

      Volume: 269 Pages: 49-54

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Comprehensive Analysis of the Fungal Microbiota of the Human External Auditory Canal Using Pyrosequencing2017

    • Author(s)
      Otomi Cho, Takashi Sugita
    • Journal Title

      Medical Mycology Journal

      Volume: 58 Issue: 1 Pages: E1-E4

    • DOI

      10.3314/mmj.16-00023

    • NAID

      130005401915

    • ISSN
      2185-6486, 2186-165X
    • Related Report
      2017 Research-status Report 2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Inhibition of Propionibacterium acnes lipase activity by the antifungal agent ketoconazole2017

    • Author(s)
      Unno M, Cho O, Sugita T
    • Journal Title

      Microbiol. Immunol

      Volume: 61 Issue: 1 Pages: 42-44

    • DOI

      10.1111/1348-0421.12464

    • NAID

      40021110140

    • Related Report
      2017 Research-status Report 2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] Low DNA sequence diversity of the intergenic spacer 1 region in the human skin commensal fungi Malassezia sympodialis and M. dermatis isolated from patients with Malassezia-associated skin diseases and healthy subjects2016

    • Author(s)
      Otomi Cho, Takashi Sugita
    • Journal Title

      Mycopathologia

      Volume: 181 Issue: 11-12 Pages: 839-842

    • DOI

      10.1007/s11046-016-0034-3

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] 皮膚マイクロバイオームStaphylococcus aureus とステロイド薬がヒト皮膚角化細胞に与える細胞障害性2019

    • Author(s)
      加藤隼平、張 音実、小山真珠、大久保友隆、中嶋拓海、杉田 隆
    • Organizer
      日本薬学会第139年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 吉草酸ベタメタゾンの皮膚マイクロバイオーム、Staphylococcus aureus に対する抗菌作用2019

    • Author(s)
      中嶋拓海、張 音実、大久保友隆、加藤隼平、杉田 隆
    • Organizer
      日本薬学会第139年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 皮膚マイクロバイオームの網羅的解析:Propidium monoazide を用いた生死の判別2019

    • Author(s)
      大久保友隆、張 音実、小笠原裕樹、杉田 隆
    • Organizer
      日本薬学会第139年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 皮膚マイクロバイオームの解析と疾患制御への応用2019

    • Author(s)
      杉田 隆
    • Organizer
      Nail disease conference seminar
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 皮膚マイクロバイオームの役割2018

    • Author(s)
      杉田 隆
    • Organizer
      日本アレルギー学会第5回総合アレルギー講習会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 皮膚マイクロバイオームと皮膚疾患2018

    • Author(s)
      杉田 隆
    • Organizer
      第335回沖縄皮膚科勉強会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] カルシニューリン阻害薬が皮膚常在菌Malassezia およびケラチノサイトに与える影響2018

    • Author(s)
      大久保友隆、張 音実、杉田 隆
    • Organizer
      第62回日本医真菌学会総会・学術集会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 皮膚マイクロバイオームの解析とその制御戦略2018

    • Author(s)
      杉田 隆
    • Organizer
      第48回北陸真菌懇談会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 皮膚マイクロバイオームとしてのマラセチア2018

    • Author(s)
      杉田 隆
    • Organizer
      第117回日本皮膚科学会総会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] アトピー性皮膚炎患者皮膚より分離したnon-Staphylococcus aureus と真菌Malassezia との相互作用2018

    • Author(s)
      大久保友隆、井上瑞菜、張 音実、杉田 隆
    • Organizer
      第62回日本医真菌学会総会・学術集会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Inhibitory Effects of Lactobacillus Culture Supernatants on Candida albicans2018

    • Author(s)
      Matsuda Y, Cho O, Ogishima D, Sugita T
    • Organizer
      20th Congress of the International Society for Human and Animal Mycology (ISHAM) 2018
    • Related Report
      2018 Annual Research Report
  • [Presentation] アトピー性皮膚炎治療薬が皮膚常在菌Malassezia に与える影響2017

    • Author(s)
      大久保友隆、張 音実、杉田 隆
    • Organizer
      日本薬学会第137年会
    • Place of Presentation
      仙台、宮城
    • Year and Date
      2017-03-27
    • Related Report
      2016 Research-status Report
  • [Presentation] 皮膚常在真菌Malassezia furfurのバイオフィルム形成と抗真菌薬耐性2017

    • Author(s)
      須藤綾子、張 音実、倉門早苗、杉田 隆
    • Organizer
      日本薬学会第137年会
    • Place of Presentation
      仙台、宮城
    • Year and Date
      2017-03-27
    • Related Report
      2016 Research-status Report
  • [Presentation] Molecular detection of Acinetobacter spp. from the skin of patients with skin diseases2017

    • Author(s)
      井上瑞菜、張 音実、杉田 隆
    • Organizer
      第90 回日本細菌学会総会
    • Place of Presentation
      仙台、宮城
    • Year and Date
      2017-03-20
    • Related Report
      2016 Research-status Report
  • [Presentation] Comprehensive analysis of the skin bacterial microbiota of healthy Japanese individuals2017

    • Author(s)
      張 音実、井上瑞菜、杉田 隆
    • Organizer
      第90 回日本細菌学会総会
    • Place of Presentation
      仙台、宮城
    • Year and Date
      2017-03-20
    • Related Report
      2016 Research-status Report
  • [Presentation] 微生物叢環境メタゲノム最前線、皮膚真菌叢解析が明らかにしたこと2017

    • Author(s)
      杉田 隆
    • Organizer
      NGS現場の会 第5回大会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] Acinetobacter ursingii培養上清のStaphylococcus spp.の細胞傷害性に対する作用2017

    • Author(s)
      井上瑞菜、張 音実、杉田 隆
    • Organizer
      第61回日本薬学会関東支部大会
    • Related Report
      2017 Research-status Report
  • [Presentation] なぜ、皮膚に微生物が存在するのか -皮膚マイクロバイオームの破綻と再構築-2017

    • Author(s)
      杉田 隆
    • Organizer
      第61回日本医真菌学会総会・学術集会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] アトピー性皮膚炎患者皮膚より分離した non-Staphylococcus aureus と真菌 Malassezia との相互作用2017

    • Author(s)
      大久保 友隆、井上 瑞菜、張 音実、杉田 隆
    • Organizer
      日本薬学会第138年会
    • Related Report
      2017 Research-status Report
  • [Presentation] 皮膚上のアシネトバクターは黄色ブドウ球菌の細胞傷害性を増悪させるか2017

    • Author(s)
      井上 瑞菜、張 音実、杉田 隆
    • Organizer
      日本薬学会第138年会
    • Related Report
      2017 Research-status Report
  • [Presentation] Skin fungal microbiome of patients with Malassezia-related skin diseases2017

    • Author(s)
      Sugita T, Cho O
    • Organizer
      15th International Congress of Mycology and Eukaryotic Microbiology, IUMS 2017
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] Malassezia microbiota and related skin diseases2016

    • Author(s)
      Sugita T
    • Organizer
      6th Asian Pacific Society for Medical Mycology Congress
    • Place of Presentation
      Bali, Indonesia
    • Year and Date
      2016-10-22
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Molecular Characterization of The Skin Fungal Microbiota in Patients with Seborrheic Dermatitis2016

    • Author(s)
      Otomi Cho, Takashi Sugita, Mami Saito, Ryoji Tsuboi, Akemi Nishikawa
    • Organizer
      6th Asian Pacific Society for Medical Mycology Congress
    • Place of Presentation
      Bali, Indonesia
    • Year and Date
      2016-10-22
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] 角栓に存在するMalasseziaの局在と抗真菌薬ケトコナゾールのリパーゼ阻害作用2016

    • Author(s)
      海野瑞季、張 音実、杉田 隆
    • Organizer
      第60回日本医真菌学会総会学術大会
    • Place of Presentation
      台東区、東京
    • Year and Date
      2016-10-01
    • Related Report
      2016 Research-status Report
  • [Presentation] ヒト表皮角化細胞に対するMalasseziaと細菌Acinetobacterの作用2016

    • Author(s)
      井上瑞菜、張 音美、齋藤磨美、坪井良治、杉田 隆
    • Organizer
      第60回日本医真菌学会総会学術大会
    • Place of Presentation
      台東区、東京
    • Year and Date
      2016-10-01
    • Related Report
      2016 Research-status Report
  • [Presentation] 皮膚におけるAcinetobacterの菌叢解析と皮膚角化細胞に及ぼす影響2016

    • Author(s)
      井上瑞菜、張音実、斎藤磨美、坪井良治、杉田 隆
    • Organizer
      第60回日本薬学会関東支部大会
    • Place of Presentation
      文京区、東京
    • Year and Date
      2016-09-17
    • Related Report
      2016 Research-status Report
  • [Presentation] Fungal microbiota2016

    • Author(s)
      Sugita T
    • Organizer
      14th Iinternational Conference of Yeasts
    • Place of Presentation
      Awajishima Island, Hyogo
    • Year and Date
      2016-09-14
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited
  • [Book] メタゲノム解析2016

    • Author(s)
      張音実、杉田隆
    • Total Pages
      227
    • Publisher
      羊土社
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2020-03-30  

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