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Characterization of induction of mucosal immune responses by protein nano-capsule

Research Project

Project/Area Number 16K08412
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Medical pharmacy
Research InstitutionSaitama Medical University

Principal Investigator

KAWANO Masaaki  埼玉医科大学, 医学部, 准教授 (30447528)

Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordspolyomavirus / simian virus 40 / virus-like particle / mucosal immune response / innate immune response / adaptive immune response / adjuvant / vaccine / antibody production / major capsid protein / VP1 / mucosal immunology / mucosal adjuvant / immune induction agent / vaccine platform / 薬学 / 免疫学 / 生体材料 / ナノ材料 / ウィルス
Outline of Final Research Achievements

Simian virus 40 (SV40) is monkey polyomavirus. SV40 capsid has icosahedral structure with 45 nm in diameter and consists only of major structural protein, VP1. In order to construct SV40 capsid, five VP1 molecules form pentamer and then 72 pentamer assembles into SV40 capsid without the need of cellular factors. Virus-like particles (VLPs) of SV40 are assembled when SV40 VP1 is expressed in insect cells by baculovirus vector. In order to analyze differences of immune responses between mouse splenocytes and mouse naso-pharynx lymphoid tissue (NALT) against SV40 VLP protein nano-capsules, SV40 VLPs were incubated with splenocytes or NALT. Also, SV40 VLP-incubated mouse immune cells were introduced to the mice to induce adaptive immune responses against SV40 VLP. Moreover, SV40 VLP binding to cellular factors that localizes on the SV40 entry pathway within the cells was analyzed. Also, in order to construct vaccines using SV40 VLPs, antigens were incorporated within the SV40 VLP.

Academic Significance and Societal Importance of the Research Achievements

粘膜ワクチンは、医師の介入を必要とせずに投与できることから、医療コストの軽減に繋がることが期待される。しかしながら、目的の抗原に対して効率よく粘膜免疫を誘導できるウイルス様粒子を用いたワクチンキャリアは未だ実用化されていない。我々の解析で、simian virus 40のウイルス様粒子は経鼻投与でも脾臓リンパ球に目的の獲得免疫を効率良く誘導することが示された。この未解明の粘膜免疫誘導機構を解明することは学術的意義があり、また、医療コストの削減に繋がるVLP粘膜ワクチンキャリアの開発は、近年問題となっている社会保障費の削減にも繋がるものと考えられるため社会的意義も十分にあるものと考えられる。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (17 results)

All 2019 2018 2017 2016

All Journal Article (6 results) (of which Peer Reviewed: 6 results,  Open Access: 4 results) Presentation (7 results) Book (3 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Dopamine regulates cytokine secretion during innate and adaptive immune responses.2019

    • Author(s)
      Kawano, M., Takagi, R., Saika, K., Matsui, M., Matsushita, S.
    • Journal Title

      International Immunology

      Volume: 30 Issue: 12 Pages: 591-606

    • DOI

      10.1093/intimm/dxy057

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Interleukin‐8 produced by T cells is under the control of dopamine signaling2018

    • Author(s)
      Matsuyama Toshiyuki、Kawano Masaaki、Takagi Rie、Nakagome Kazuyuki、Chikamatsu Kazuaki、Matsushita Sho
    • Journal Title

      Clinical and Experimental Neuroimmunology

      Volume: 9 Issue: 4 Pages: 251-257

    • DOI

      10.1111/cen3.12472

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Protein kinase C activation via serotonin receptor induces IL-8 in antigen-presenting cells stimulated with diazinon2018

    • Author(s)
      Nishizawa K, Takagi R, Kawano M, Matsushita S.
    • Journal Title

      Current Topics in Toxicology

      Volume: 14 Pages: 41-52

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Acetylcholine stored in dendritic cells plays a role in the differentiation of naive CD4+ T cells into a Th1 phenotype2017

    • Author(s)
      Matsueda H, Kawano M, Takagi R, Koshimuzu K, Matsushita S.
    • Journal Title

      Clinical and Experimental Neuroimmunology

      Volume: 8 Issue: 2 Pages: 1-9

    • DOI

      10.1111/cen3.12427

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Signaling via FcεRI stimulates human immature dendritic cells to induce Th1 differentiation via IL-12: Novel evidence of a feedback loop of allergic responses mediated by dendritic cells2017

    • Author(s)
      Itano A, Kawano M, Takagi R, Tokuyama K, Matsushita S.
    • Journal Title

      Current Trends in Immunology

      Volume: 18 Pages: 57-65

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Identification of the novel 3' UTR sequences of human IL-21 mRNA as potential targets of miRNAs2017

    • Author(s)
      Enomoto Y., Takagi R., Naito Y., Kiniwa T., Tanaka Y., Hamada-Tsutsumi S., Kawano M., Matsushita S., Ochiya T., Miyajima A.
    • Journal Title

      Scientific Reports

      Volume: 7 Issue: 1 Pages: 1-9

    • DOI

      10.1038/s41598-017-07853-x

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Tannic acid affects dopamine receptors, regulates immune responses, and ameliorates experimentally induced colitis2018

    • Author(s)
      Masaaki Kawano, Kikue Saika, Rie Takagi, Masanori Matsui, Sho Matsushita
    • Organizer
      The 47th Annual Meeting of The Japanese Society for Immunology, 2018
    • Related Report
      2018 Annual Research Report
  • [Presentation] 初期免疫応答におけるドーパミンによる 免疫関連遺伝子発現の解析2017

    • Author(s)
      川野 雅章, 高木 理英, 松下 祥
    • Organizer
      第66回日本アレルギー学会学術大会
    • Related Report
      2017 Research-status Report
  • [Presentation] 樹状細胞由来アセチルコリンによるTh1分化誘導2017

    • Author(s)
      松枝 秀世, 川野 雅章, 高木 理英, 松下 祥
    • Organizer
      第66回日本アレルギー学会学術大会
    • Related Report
      2017 Research-status Report
  • [Presentation] T細胞によるIL-8産生とドーパミンシグナル2017

    • Author(s)
      松山 敏之, 川野 雅章, 高木 理英, 松下 祥
    • Organizer
      第66回日本アレルギー学会学術大会
    • Related Report
      2017 Research-status Report
  • [Presentation] Dopamine fluctuates cytokine secretion in innate and adaptive immune responses2017

    • Author(s)
      Masaaki Kawano, Rie Takagi, Kikue Saika, Masanori Matsui, Sho Matsushita
    • Organizer
      The 46th Annual Meeting of The Japanese Society for Immunology, 2017
    • Related Report
      2017 Research-status Report
  • [Presentation] Dopamine modulates cytokine secretion in early immune responses as well as the generation of T-helper subsets in adaptive responses2016

    • Author(s)
      KAWANO Masaaki, TAKAGI Rie, MATSUSHITA Sho
    • Organizer
      The 45th Annual Meeting of The Japanese Society for Immunology, 2016
    • Place of Presentation
      沖縄コンベンションセンター、ラグナガーデンホテル (沖縄県 宜野湾市)
    • Year and Date
      2016-12-05
    • Related Report
      2016 Research-status Report
  • [Presentation] ドーパミンによる炎症性免疫応答転調機構の解析2016

    • Author(s)
      川野 雅章, 高木 理英, 金子 篤, 松下 祥
    • Organizer
      第65回日本アレルギー学会学術大会
    • Place of Presentation
      東京国際フォーラム (東京都 千代田区)
    • Year and Date
      2016-06-17
    • Related Report
      2016 Research-status Report
  • [Book] Magnetic Nanoparticles for Medical Diagnostics2018

    • Author(s)
      Masaaki Kawano, Mamoru Hatakeyama, Masanori Matsui, Hiroshi Handa.
    • Total Pages
      150
    • Publisher
      IOP Publishing Ltd
    • ISBN
      9780750315821
    • Related Report
      2018 Annual Research Report
  • [Book] WHAT’S NEW in 皮膚科学2018

    • Author(s)
      土田 哲也、中村 晃一郎、高木 理英、川野 雅章、松下 祥
    • Total Pages
      228
    • Publisher
      メディカルレビュー社
    • ISBN
      9784779220722
    • Related Report
      2018 Annual Research Report
  • [Book] Design and Development of New Nanocarriers2017

    • Author(s)
      Masaaki Kawano, Masanori Matsui, Hiroshi Handa
    • Total Pages
      741
    • Publisher
      Elsevier Inc.
    • Related Report
      2017 Research-status Report
  • [Patent(Industrial Property Rights)] 活性化T細胞からのIL-8産生を抑制するための組成物2017

    • Inventor(s)
      松下 祥, 川野 雅章
    • Industrial Property Rights Holder
      松下 祥, 川野 雅章
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2017-106234
    • Filing Date
      2017
    • Related Report
      2017 Research-status Report

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Published: 2016-04-21   Modified: 2020-03-30  

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