Morphofunctional analyses of membrane skeletal proteins using MPP family gene mutant mice
| Project/Area Number |
16K08463
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General anatomy (including histology/embryology)
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| Research Institution | Shinshu University |
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Principal Investigator |
Terada Nobuo 信州大学, 学術研究院保健学系, 教授 (60293461)
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 末梢神経 / 膜骨格 / 髄鞘 / 蛋白複合体 / 足場蛋白 / 接着分子 / プロテイン４．１ / ミエリン / 細胞内シグナル / 末梢神経系 / 凍結技法 / 細胞膜 / シグナル伝達 / 膜骨格蛋白 |
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| Outline of Final Research Achievements |
Under 4.1G deficiency, cell adhesion molecule (CADM)4, membrane palmitoylated protein (MPP)6, and Lin7 were disappeared in Schmidt-Lanterman incisures in peripheral nervous system (PNS) myelinated nerve fivers. 4.1G-deficient mouse had impairment of moter activity with decreased nerve conduction velocity. We produced novel MPP6-deficient mice, showing disappearnce of Lin7 as well as remaining of 4.1G and CADM4.
Thus, process of the 4.1G-CADM4-MPP6-Lin7 molecular complex in PNS was clarified.
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| Academic Significance and Societal Importance of the Research Achievements |
末梢神経における4.1G蛋白複合体に新たにシグナル蛋白であるLin7を含み、4.1GがMPP6とCADM4と合わせてその輸送も主導することが明らかとなり、さらに4.1G欠損における形態と生理機能にも異常を認めたことから、4.1Gの複合体蛋白の運搬と局在化による髄鞘形成への制御の役割が示唆された。
ヒト末梢神経系にもCADM4や4.1Gが存在していることから、マウスで見出している膜骨格構造の破綻が、ヒトのシャルコー・マリー・トゥース病の原因や病態を解明する一助となることが示唆された。
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Report
(4 results)
Research Products
(24 results)
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[Journal Article] Neuregulin-1 type III knockout mice exhibit delayed migration of Schwann cell precursors.2017
Author(s)
Miyamoto Y, Torii T, Tanoue A, Kawahara K, Arai M, Tsumura H, Ogata T, Nagao M, Terada N, Yamamoto M, Takashima S, Yamauchi J
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Journal Title
Biochemical and Biophysical Research Communication,
Volume: 486
Issue: 2
Pages: 506-513
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Presentation] Function of a membrane skeletal protein, 4.1G, in myelin formation in the peripheral nervous system.2017
Author(s)
Terada N, Saitoh Y, Ohno N, Yamauchi J, Sakamoto T
Organizer
The 12th China-Japan Joint Seminar on Histochemistry and Cytochemistry, Zhangjiakou, Hebei, China,
Related Report
Int'l Joint Research / Invited
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