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Elucidation of the involvement of calpain and cytoskeletal proteins in the signal transduction of abnormal vascular smooth muscle contraction

Research Project

Project/Area Number 16K08496
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field General physiology
Research InstitutionYamaguchi University

Principal Investigator

KISHI Hiroko  山口大学, 大学院医学系研究科, 准教授 (40359899)

Co-Investigator(Kenkyū-buntansha) 張 影  山口大学, 大学院医学系研究科, 講師 (10711260)
森田 知佳  山口大学, 大学院医学系研究科, 助教 (70763796)
小林 誠  山口大学, 大学院医学系研究科, 教授 (80225515)
Research Collaborator LYU Bochao  
ZHANG Min  
LU Qian  
LI Nan  
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords血管平滑筋 / 異常収縮 / 細胞骨格 / カルパイン / 血管異常収縮 / 機能プロテオミクス / 生理学
Outline of Final Research Achievements

Whereas normal vascular contraction which regulates normal blood pressure is cytosolic Ca2+-dependent, abnormal vascular contraction which plays a critical role in the pathophysiology of sudden and fatal ischemic diseases such as myocardial infarction and post-SAH cerebral vasospasm, is Ca2+-independent, that is, it does not require cytosolic Ca2+ elevation. In this research project, we attempted to clarify the involvement of calpain and cytoskeletal proteins in the signal transduction of abnormal vascular smooth muscle contraction. We found that sphingosylphosphorylcholine (SPC) induced calpain activation as well as abnormal contraction in vascular smooth muscle. We also revealed the cleavage site of V1 by calpain using mass spectrometry. Furthermore, we found a calpain inhibitor inhibited abnormal vascular contraction without affecting normal vascular contraction.

Academic Significance and Societal Importance of the Research Achievements

学術的意義:血管異常収縮のシグナル伝達機構は正常収縮と異なりカルシウム非依存性であるが詳細は不明であった。本研究は血管異常収縮シグナル伝達において新規の機構を解明したものである。
社会的意義:血管異常収縮が引き起こす心筋梗塞やくも膜下出血後脳血管攣縮はしばしば難治性である。本研究はこれらの疾患の特効薬開発のための新規のターゲットとしてカルパインおよび細胞骨格V1を示唆するものである。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (27 results)

All 2019 2018 2017 2016

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 4 results,  Acknowledgement Compliant: 2 results) Presentation (21 results) (of which Int'l Joint Research: 2 results) Book (1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Add-on therapy with traditional Chinese medicine: An efficacious approach for lipid metabolism disorders2018

    • Author(s)
      Ying Zhang, Hiroko Kishi, Sei Kobayashi
    • Journal Title

      Pharmacological Research

      Volume: 134 Pages: 200-211

    • DOI

      10.1016/j.phrs.2018.06.004

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The nutritional characteristics of the hypotensive WASHOKU-modified DASH diet: a sub-analysis of the DASH-JUMP study2018

    • Author(s)
      Atsuko Kawamura, Katsuko Kajiya, Hiroko Kishi, Junko Inagaki, Makoto Mitarai, Hiroshi Oda, Seiji Umemoto, Sei Kobayashi
    • Journal Title

      Current Hypertension Reviews

      Volume: 14 Issue: 1 Pages: 1-10

    • DOI

      10.2174/1573402114666180405100430

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Omega-3 and omega-6 DPA equally inhibit the sphingosylphosphorylcholine-induced Ca2+-sensitization of vascular smooth muscle contraction via inhibiting Rho-kinase activation and translocation.2017

    • Author(s)
      Ying Zhang, Min Zhang, Bochao Lyu, Hiroko Kishi and Sei Kobayashi
    • Journal Title

      Scientific Report

      Volume: 7 Issue: 1 Pages: 1-14

    • DOI

      10.1038/srep36368

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Effects of the DASH-JUMP dietary intervention in Japanese participants with high-normal blood pressure and stage 1 hypertension: an open-label single-arm trial.2016

    • Author(s)
      Kawamura A, Kajiya K, Kishi H, Inagaki J, Mitarai M, Oda H, Umemoto S, Kobayashi S.
    • Journal Title

      Hypertens Res

      Volume: 39 Issue: 11 Pages: 777-785

    • DOI

      10.1038/hr.2016.76

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] The involvement of calpain in abnormal vascular smooth muscle contraction induced by SPC and U466192019

    • Author(s)
      Hiroko Kishi, Qian Lu, Tomoka Morita, Ying Zhang, Bochao Lyu, Min Zhang, Nan Li, Sei Kobayashi
    • Organizer
      9th Federation of the Asian and Oceanian Physiological Societies (FAOPS) Congress
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Direct Fyn-paxillin binding controls migration of coronary artery smooth muscle cells2019

    • Author(s)
      Ying Zhang, Min Zhang, Bochao Lyu, Hiroko Kishi, Tomoka Morita, Qian Lu, Nan Li, Sei Kobayashi
    • Organizer
      9th Federation of the Asian and Oceanian Physiological Societies (FAOPS) Congress
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 血管平滑筋のCa2+非依存性収縮と細胞遊走を制御する新規シグナル分子の同定2018

    • Author(s)
      張 影、松永 一真、岡本 嵩史、呂 博超、張 敏、森田 知佳、岸 博子、小林 誠
    • Organizer
      筋生理の集い
    • Related Report
      2018 Annual Research Report
  • [Presentation] SPC/Fyn/ROK系による血管平滑筋収縮のCa2+-sensitizationにおける、カルパインの役割2018

    • Author(s)
      岸 博子、張 影、森田 知佳、呂 博超、張 敏、路 倩、小林 誠
    • Organizer
      第60回日本平滑筋学会総会
    • Related Report
      2018 Annual Research Report
  • [Presentation] ドコサペンタ塩酸(DPA)は、n-3とn-6の両系統とも同じ力価で、Rhoキナーゼの細胞内移動と活性化を抑制する事によって血管異常収縮を抑制する2018

    • Author(s)
      張 影、張 敏、呂 博超、岸 博子、森田 知佳、路 倩、小林 誠
    • Organizer
      第60回日本平滑筋学会総会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 活性型FynチロシンキナーゼのパキシリンN末端への直接結合は血管平滑筋細胞のストレスファイバー形成と細胞遊走を制御する2018

    • Author(s)
      張 影、岸 博子、呂 博超、張 敏、森田 知佳、路 倩、小林 誠
    • Organizer
      第60回日本平滑筋学会総会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 血管攣縮と癌細胞遊走の両方を阻止できる新規の水溶性食品成分の発見2018

    • Author(s)
      浅田充夫、呂 博超、張 敏、張 影、岸 博子、森田 知佳、小林 誠
    • Organizer
      第60回日本平滑筋学会総会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Compound A suppresses the Fyn/Rho-kinase mediated Ca2+-sensitization of vascular smooth muscle contraction2018

    • Author(s)
      呂 博超、張 敏、張 影、岸 博子、森田 知佳、小林 誠
    • Organizer
      第60回日本平滑筋学会総会
    • Related Report
      2018 Annual Research Report
  • [Presentation] The novel compound from soybean strongly and selectively inhibits the Rho-kinase-mediated Ca2+-sensitization of vascular smooth muscle contraction2018

    • Author(s)
      張 敏、張 影、呂 博超、高柿 了大、岸 博子、加治屋 勝子、森田 知佳、小林 誠
    • Organizer
      第60回日本平滑筋学会総会
    • Related Report
      2018 Annual Research Report
  • [Presentation] エイコサペンタエン酸(EPA)による肺動脈高血圧病態の改善効果2018

    • Author(s)
      倉原 琳、平石 敬三、張 影、山村 彩、岸 博子、小林 誠、井上 隆司
    • Organizer
      第60回日本平滑筋学会総会
    • Related Report
      2018 Annual Research Report
  • [Presentation] The involvement of membrane rafts in the signal transduction of abnormal vascular smooth muscle contraction mediated by SPC/Fyn/ROK pathway: Part II2018

    • Author(s)
      Hiroko Kishi, Miki Kajima, Katsuko Kajiya, Ying Zhang, Tomoka Morita, Bochao Lyu, Min Zhang, Sei Kobayashi
    • Organizer
      第95回日本生理学会大会
    • Related Report
      2017 Research-status Report
  • [Presentation] Fyn-mediated tyrosine phosphorylation of a novel signaling molecule, paxillin controls stress fiber formation and migration of cancer cells2018

    • Author(s)
      Shiori Yokobayashi, Ying Zhang, Takafumi Okamoto, Tomoka Morita, Hiroko Kishi, Takeshi Yamamoto, Sei Kobayashi
    • Organizer
      第95回日本生理学会大会
    • Related Report
      2017 Research-status Report
  • [Presentation] Omega-3 and omega-6 docosapentaenoic acid (DPA) equally inhibit the Ca2+-sensitization of abnormal vascular contractions via inhibiting the Rho-kinase activation and translocation.2018

    • Author(s)
      Ying Zhang, Min Zhang, Bochao Lyu, Hiroko Kishi, Tomoka Morita, and Sei Kobayashi
    • Organizer
      第95回日本生理学会大会
    • Related Report
      2017 Research-status Report
  • [Presentation] The involvement of membrane rafts in the signal transduction of abnormal vascular smooth muscle contraction mediated by SPC/Fyn/ROK pathway2017

    • Author(s)
      Hiroko Kishi, Ying Zhang, Katsuko Kajiya, Tomoka Morita, Bochao Lyu, Min Zhang, Xiaoyan Li, Sei Kobayashi
    • Organizer
      第94回日本生理学会大会
    • Place of Presentation
      アクトシティ浜松 (浜松)
    • Year and Date
      2017-03-28
    • Related Report
      2016 Research-status Report
  • [Presentation] Paxillin as a novel signaling molecule regulates actin stress fiber formation and migration of vascular smooth muscle cells by its N-terminus binding to active Fyn2017

    • Author(s)
      Ying Zhang, Hiroko Kishi, Katsuko Kajiya, Tomoka Morita, Sei Kobayashi
    • Organizer
      第94回日本生理学会大会
    • Place of Presentation
      アクトシティ浜松 (浜松)
    • Year and Date
      2017-03-28
    • Related Report
      2016 Research-status Report
  • [Presentation] Tyrosine phosphorylation of paxillin regulates migration and invasion of cancer cells2017

    • Author(s)
      Takafumi Okamoto, Ying Zhang, Kazumasa Matsunaga, Shiori Yokobayashi, Tomoka Morita, Hiroko Kishi, Sei Kobayashi
    • Organizer
      第94回日本生理学会大会
    • Place of Presentation
      アクトシティ浜松 (浜松)
    • Year and Date
      2017-03-28
    • Related Report
      2016 Research-status Report
  • [Presentation] スフィンゴシルホスホリルコリンが誘発する血管平滑筋異常収縮シグナル分子の細胞内局在の変化の検討2017

    • Author(s)
      岸 博子、張 影、加治屋 勝子、森田 知佳、呂 博超、張 敏、小林 誠
    • Organizer
      第59回日本平滑筋学会総会
    • Related Report
      2017 Research-status Report
  • [Presentation] パキシリンはN末端と活性型Fynとの相互作用により血管平滑筋細胞遊走を制御する2017

    • Author(s)
      張 影、岸 博子、森田 知佳、呂 博超、張 敏、横林志織、小林 誠
    • Organizer
      第59回日本平滑筋学会総会
    • Related Report
      2017 Research-status Report
  • [Presentation] The novel compound from soybean strongly and selectively inhibits the Rho-kinase-mediated Ca2+-sensitization of vascular smooth muscle contraction2017

    • Author(s)
      張 敏、張 影、呂 博超、岸 博子、加治屋 勝子、森田 知佳、小林 誠
    • Organizer
      第59回日本平滑筋学会総会
    • Related Report
      2017 Research-status Report
  • [Presentation] Compound X inhibits the ROK-mediated Ca2+-sensitization of vascular smooth muscle contraction induced by a spasmogen, sphingosylphosphorylcholine2016

    • Author(s)
      Bochao Lyu, Ying Zhang, Hiroko Kishi, Tomoka Morita, Sei Kobayashi
    • Organizer
      第68回日本生理学会中国四国地方会
    • Place of Presentation
      岡山大学Junko Fukutakeホール (岡山)
    • Year and Date
      2016-11-05
    • Related Report
      2016 Research-status Report
  • [Presentation] The novel compound from soybean has a strong inhibitory effect on the Rho-kinase-mediated Ca2+-sensitization of vascular smooth muscle contraction2016

    • Author(s)
      張 敏、張 影、呂 博超、岸 博子、森田知佳、小林 誠
    • Organizer
      筋生理の集い
    • Place of Presentation
      東京慈恵会医科大学 (東京)
    • Related Report
      2016 Research-status Report
  • [Book] Chapter 13, The pivotal role of cholesterol and membrane lipid rafts in the Ca2+-sensitization of vascular smooth muscle contraction leading to vasospasm, Muscle Contraction and Cell Motility: Fundamentals and Developments, edited by Haruo Sugi2016

    • Author(s)
      Ying Zhang, Hiroko Kishi, Katsuko Kajiya, Tomoka Morita, Sei Kobayashi
    • Total Pages
      454
    • Publisher
      Pan Stanford
    • Related Report
      2016 Research-status Report
  • [Patent(Industrial Property Rights)] 安息香酸誘導体を含む血管攣縮抑制剤、食品及び食品添加剤2017

    • Inventor(s)
      小林 誠、岸 博子、 高松日出子、石川敦子
    • Industrial Property Rights Holder
      山口大学、株式会社タカマ
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2017-237056
    • Filing Date
      2017
    • Related Report
      2017 Research-status Report

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Published: 2016-04-21   Modified: 2020-03-30  

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