Identification of the glycoproteins-induced bone formation mechanisms at bone resorption lacunae
| Project/Area Number |
16K08506
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General physiology
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| Research Institution | Keio University |
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Principal Investigator |
Kuroda Yukiko 慶應義塾大学, 医学部(信濃町), 助教 (70455343)
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 骨形成 / 糖鎖 / 骨吸収窩 / 骨芽細胞 / 破骨細胞 / 血管内皮細胞 / マトリックスプロテアーゼ / 糖タンパク質 / 細胞間相互作用 / 糖鎖修飾 |
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| Outline of Final Research Achievements |
Abundant glycoproteins exist in bone resorption lacunae, whereas their physiological roles remain unknown. During endochondral ossification, localization of matrix metalloproteinase-9 (MMP-9) was significantly different according to its glycosylation state. Non-glycosylated MMP-9 is exclusively present around tips of capillary vessels in the chondro-osseous junction. On the other hand, sialylated MMP-9 exists in osteoclasts and resorption lacunae accompanied with mature osteoblasts. These results suggest that non-glycosylated MMP-9 attracts endothelial cells, while sialylated MMP-9 enhances the osteoblast migration and bone formation at bone resorption lacunae.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究では、骨吸収窩に存在する糖タンパク質に注目し、その糖鎖修飾の骨形成における生理的意義を解明しようと挑戦する点に高い新規性・独創性がある。骨吸収部位での骨形成機構に関する知見は数多く報告されているが、次段階である骨形成誘導の有無で骨吸収窩を分類し、「それぞれの骨吸収窩によって異なる機構が働いている」という発想の研究はなく、その役割の違いを見つけようとする発想が独創的である。もし、これらの糖タンパク質が骨形態形成機構に関与していれば、生体内の骨形成を新しい視点から見ることができるだけでなく、骨形成を促す技術としても応用できるため、医療に大きな貢献ができると考えられる。
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Report
(4 results)
Research Products
(15 results)
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[Journal Article] Bone2019
Author(s)
Matsuo K, Ji S, Miya A, Yoda M, Hamada Y, Tanaka T, Takao-Kawabata R, Kawaai K, Kuroda Y, Shibata S.
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Journal Title
Innervation of the tibial epiphysis through the intercondylar foramen.
Volume: 120
Pages: 297-304
DOI
Related Report
Peer Reviewed / Open Access
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